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          pCas-Guide-EF1a-CD4

          價格:1000元

          聯系方式:I47-825O-882O

          買家導航

          載體基本信息

          出品公司: ORIGENE
          載體名稱: pCas-Guide-EF1a-CD4
          質粒類型: CRISPR/Cas9載體;基因敲除載體;哺乳動物細胞載體
          高拷貝/低拷貝: 高拷貝
          插入位點: 限制性內切酶,多克隆位點
          啟動子: U6
          載體大小: 11006 bp
          5' 測序引物及序列: 5’ gatcgATGGGAGGTGGTATGGGAGGg 3’
          3' 測序引物及序列: 5’ aaaacCCTCCCATACCACCTCCCATc 3’
          載體標簽: Myc(C-ter)
          載體抗性: 氨芐青霉素
          篩選標記: --
          克隆菌株: Stbl3
          宿主細胞(系): 哺乳動物細胞
          備注: --
          產品目錄號: GE100022
          穩定性: 瞬表達
          組成型/誘導型: 組成型
          病毒/非病毒: 非病毒

          載體質粒圖譜和多克隆位點信息

          pCas-Guide-EF1a-CD4載體圖譜



          載體簡介

          pCas-Guide-EF1a-CD4載體特征
          All-in-one vector
          
          Expresses human codon-optimized Cas9, C-terminal Myc-DDK tagged
          BamH I and BsmB I sites are used for genomic target sequence cloning
          Expresses gRNA containing the inserted target sequence and the gRNA scaffold
          EF1a promoter driven CD4 to enrich transfected cells
          CF3 primer (GE100008) can be used to sequence the target sequence after cloning
          

          載體序列

          LOCUS       Exported File          11006 bp ds-DNA    circular SYN 23-七月-2016
          DEFINITION  Mammalian vector for co-expressing a guide RNA (gRNA) together with 
                      Cas9 and CD4, for genome editing using the S. pyogenes CRISPR/Cas9 
                      system.
          ACCESSION   .
          VERSION     .
          KEYWORDS    pCas-Guide-EF1a-CD4
          SOURCE      synthetic DNA construct
            ORGANISM  synthetic DNA construct
          REFERENCE   1  (bases 1 to 11006)
            AUTHORS   OriGene
            TITLE     Direct Submission
            JOURNAL   Exported 2016-07-23 from SnapGene Viewer 1.5.3
                      http://www.snapgene.com
          FEATURES             Location/Qualifiers
               source          1..11006
                               /organism="synthetic DNA construct"
                               /lab_host="Mammalian Cells"
                               /mol_type="other DNA"
               promoter        90..330
                               /note="U6"
                               /note="RNA polymerase III promoter for human U6 snRNA"
                               /note="color: #000000; direction: RIGHT"
               misc_RNA        384..459
                               /note="gRNA scaffold"
                               /note="guide RNA scaffold for the Streptococcus pyogenes 
                               CRISPR/Cas9 system"
                               /note="color: #00ccff; direction: RIGHT"
               terminator      460..465
                               /note="polIII terminator"
                               /note="RNA polymerase III transcription terminator"
                               /note="color: #ffffff"
               promoter        480..1658
                               /note="EF-1-alpha promoter"
                               /note="strong constitutive promoter for human elongation 
                               factor EF-1-alpha"
                               /note="color: #000000; direction: RIGHT"
               intron          711..1649
                               /note="EF-1-alpha intron A"
                               /note="intron upstream of the start codon of human 
                               EF-1-alpha"
                               /note="color: #------"
               CDS             1679..2950
                               /codon_start=1
                               /product="truncated human CD4 cell surface glycoprotein 
                               lacking the cytoplasmic tail"
                               /note="CD4 (truncated)"
                               /note="This feature has 2 segments:
                               ???1:?1679?..?1753?/?#ff6600?/?signal?peptide
                               ???2:?1754?..?2950?/?#ff6600
                               Cleavage site after base 1753"
                               /translation="MNRGVPFRHLLLVLQLALLPAATQGKKVVLGKKGDTVELTCTASQ
                               KKSIQFHWKNSNQIKILGNQGSFLTKGPSKLNDRADSRRSLWDQGNFPLIIKNLKIEDS
                               DTYICEVEDQKEEVQLLVFGLTANSDTHLLQGQSLTLTLESPPGSSPSVQCRSPRGKNI
                               QGGKTLSVSQLELQDSGTWTCTVLQNQKKVEFKIDIVVLAFQKASSIVYKKEGEQVEFS
                               FPLAFTVEKLTGSGELWWQAERASSSKSWITFDLKNKEVSVKRVTQDPKLQMGKKLPLH
                               LTLPQALPQYAGSGNLTLALEAKTGKLHQEVNLVVMRATQLQKNLTCEVWGPTSPKLML
                               SLKLENKEAKVSKREKAVWVLNPEAGMWQCLLSDSGQVLLESNIKVLPTWSTPVQPMAL
                               IVLGGVAGLLLFIGLGIFFCVRCRH"
               enhancer        3560..3863
                               /note="CMV enhancer"
                               /note="human cytomegalovirus immediate early enhancer"
                               /note="color: #000000"
               promoter        3864..4067
                               /note="CMV promoter"
                               /note="human cytomegalovirus (CMV) immediate early 
                               promoter"
                               /note="color: #000000; direction: RIGHT"
               promoter        4093..4111
                               /note="T7 promoter"
                               /note="promoter for bacteriophage T7 RNA polymerase"
                               /note="color: #ffffff; direction: RIGHT"
               CDS             4170..8273
                               /codon_start=1
                               /locus_tag="
                               "
                               /product="Cas9 (Csn1) endonuclease from the?Streptococcus 
                               pyogenes?Type II CRISPR/Cas system"
                               /note="Cas9"
                               /note="generates RNA-guided double strand breaks in DNA"
                               /note="color: #993366"
                               /protein_id="
                               "
                               /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                               NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                               FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                               FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                               ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                               IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                               QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                               RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                               NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                               FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                               SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                               LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                               QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                               HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                               LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                               SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                               VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                               NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                               EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                               ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                               TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                               LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                               ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                               DATLIHQSITGLYETRIDLSQLGGD"
               CDS             8274..8294
                               /codon_start=1
                               /locus_tag="
                               "
                               /product="nuclear localization signal of SV40 large T 
                               antigen"
                               /note="SV40 NLS"
                               /note="
                               "
                               /note="color: #993366"
                               /protein_id="
                               "
                               /translation="PKKKRKV"
               CDS             8301..8321
                               /codon_start=1
                               /locus_tag="
                               "
                               /product="nuclear localization signal of SV40 large T 
                               antigen"
                               /note="SV40 NLS"
                               /note="
                               "
                               /note="color: #993366"
                               /protein_id="
                               "
                               /translation="PKKKRKV"
               CDS             8343..8372
                               /codon_start=1
                               /product="Myc (human c-Myc oncogene) epitope tag"
                               /note="Myc"
                               /note="color: #cc99b2"
                               /translation="EQKLISEEDL"
               CDS             8391..8414
                               /codon_start=1
                               /product="FLAG(R) epitope tag, followed by an enterokinase 
                               cleavage site"
                               /note="FLAG"
                               /note="color: #cc99b2"
                               /translation="DYKDDDDK"
               polyA_signal    8461..9083
                               /note="hGH poly(A) signal"
                               /note="human growth hormone polyadenylation signal"
                               /note="color: #a6acb3"
               rep_origin      complement(9204..9792)
                               /direction=LEFT
                               /note="ori"
                               /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                               replication"
                               /note="color: #ffff00"
               CDS             complement(9967..10827)
                               /codon_start=1
                               /gene="bla"
                               /product="beta-lactamase"
                               /note="AmpR"
                               /note="confers resistance to ampicillin, carbenicillin, and
                               related antibiotics"
                               /note="This feature has 2 segments:
                               ???1:??9967?..?10758?/?#ccffcc
                               ???2:?10759?..?10827?/?#ccffcc?/?signal?sequence
                               Cleavage site after base 10758"
                               /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                               ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                               PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                               EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                               LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                               LIKHW"
          ORIGIN
                  1 gaattcccca gtggaaagac gcgcaggcaa aacgcaccac gtgacggagc gtgaccgcgc
                 61 gccgagcgcg cgccaaggtc gggcaggaag agggcctatt tcccatgatt ccttcatatt
                121 tgcatatacg atacaaggct gttagagaga taattagaat taatttgact gtaaacacaa
                181 agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt
                241 taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt
                301 tcttgggttt atatatcttg tggaaaggac gcgggatcca ctggaccagg cagcagcgtc
                361 agaagacttt tttggaacgt ctcgttttag agctagaaat agcaagttaa aataaggcta
                421 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttggtg tacacgtgag
                481 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
                541 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
                601 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
                661 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
                721 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
                781 cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg
                841 agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc
                901 ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt
                961 tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc
               1021 aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg
               1081 cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag
               1141 cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg
               1201 gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag
               1261 ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga
               1321 cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt
               1381 cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt
               1441 agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg
               1501 agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat
               1561 tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag
               1621 tggttcaaag tttttttctt ccatttcagg tgtcgtgact atagggcggc cggacgtgat
               1681 gaaccgggga gtccctttta ggcacttgct tctggtgctg caactggcgc tcctcccagc
               1741 agccactcag ggaaagaaag tggtgctggg caaaaaaggg gatacagtgg aactgacctg
               1801 tacagcttcc cagaagaaga gcatacaatt ccactggaaa aactccaacc agataaagat
               1861 tctgggaaat cagggctcct tcttaactaa aggtccatcc aagctgaatg atcgcgctga
               1921 ctcaagaaga agcctttggg accaaggaaa ctttcccctg atcatcaaga atcttaagat
               1981 agaagactca gatacttaca tctgtgaagt ggaggaccag aaggaggagg tgcaattgct
               2041 agtgttcgga ttgactgcca actctgacac ccacctgctt caggggcaga gcctgaccct
               2101 gaccttggag agcccccctg gtagtagccc ctcagtgcaa tgtaggagtc caaggggtaa
               2161 aaacatacag ggggggaaga ccctctccgt gtctcagctg gagctccagg atagtggcac
               2221 ctggacatgc actgtcttgc agaaccagaa gaaggtggag ttcaaaatag acatcgtggt
               2281 gctagctttc cagaaggcct ccagcatagt ctataagaaa gagggggaac aggtggagtt
               2341 ctccttccca ctcgccttta cagttgaaaa gctgacgggc agtggcgagc tgtggtggca
               2401 ggcggagagg gcttcctcct ccaagtcttg gatcaccttt gacctgaaga acaaggaagt
               2461 gtctgtaaaa cgggttaccc aggaccctaa gctccagatg ggcaagaagc tcccgctcca
               2521 cctcaccctg ccccaggcct tgcctcagta tgctggctct ggaaacctca ccctggccct
               2581 tgaagcgaaa acaggaaagt tgcatcagga agtgaacctg gtggtgatga gagccactca
               2641 gctccagaaa aatttgacct gtgaggtgtg gggacccacc tcccctaagc tgatgctgag
               2701 cttgaaactg gagaacaagg aggcaaaggt ctcgaagcgg gagaaggcgg tgtgggtgct
               2761 gaaccctgag gcggggatgt ggcagtgtct gctgagtgac tcgggacagg tcctgctgga
               2821 atccaacatc aaggttctgc ccacatggtc caccccggtg cagccaatgg ccctgattgt
               2881 gctggggggc gtcgccggcc tcctgctttt cattgggcta ggcatcttct tctgtgtcag
               2941 gtgccggcac accggttagg atatcagatc cccgggatgc agaaattgat gatctattaa
               3001 acaataaaga tgtccactaa aatggaagtt tttcctgtca tactttgtta agaagggtga
               3061 gaacagagta cctacatttt gaatggaagg attggagcta cgggggtggg ggtggggtgg
               3121 gattagataa atgcctgctc tttactgaag gctctttact attgctttat gataatgttt
               3181 catagttgga tatcataatt taaacaagca aaaccaaatt aagggccagc tcattcctcc
               3241 cactcatgat ctatagatct atagatctct cgtgggatca ttgtttttct cttgattccc
               3301 actttgtggt tctaagtact gtggtttcca aatgtgtcag tttcatagcc tgaagaacga
               3361 gatcagcagc ctctgttcca catacacttc attctcagta ttgttttgcc aagttctaat
               3421 tccatcagaa gctggtcgag atccggaacc cttaatataa cttcgtataa tgtatgctat
               3481 acgaagttat taggtccact agttattaat agtaatcaat tacggggtca ttagttcata
               3541 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
               3601 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
               3661 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
               3721 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
               3781 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
               3841 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
               3901 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
               3961 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
               4021 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
               4081 gtcagaattt tgtaatacga ctcactatag ggcggccggg aattcgtcga ctggaaccgg
               4141 taccgaggag atctgccgcc gcgatcgcca tggataagaa atactcaata ggactggata
               4201 ttggcacaaa tagcgtcgga tgggctgtga tcactgatga atataaggtt ccttctaaaa
               4261 agttcaaggt tctgggaaat acagaccgcc acagtatcaa aaaaaatctt ataggggctc
               4321 ttctgtttga cagtggagag acagccgaag ctactagact caaacggaca gctaggagaa
               4381 ggtatacaag acggaagaat aggatttgtt atctccagga gattttttca aatgagatgg
               4441 ccaaagtgga tgatagtttc tttcatagac ttgaagagtc ttttttggtg gaagaagaca
               4501 agaagcatga aagacatcct atttttggaa atatagtgga tgaagttgct tatcacgaga
               4561 aatatccaac tatctatcat ctgagaaaaa aattggtgga ttctactgat aaagccgatt
               4621 tgcgcctgat ctatttggcc ctggcccaca tgattaagtt tagaggtcat tttttgattg
               4681 agggcgatct gaatcctgat aatagtgatg tggacaaact gtttatccag ttggtgcaaa
               4741 cctacaatca actgtttgaa gaaaacccta ttaacgcaag tggagtggat gctaaagcca
               4801 ttctttctgc aagattgagt aaatcaagaa gactggaaaa tctcattgct cagctccccg
               4861 gtgagaagaa aaatggcctg tttgggaatc tcattgcttt gtcattgggt ttgaccccta
               4921 attttaaatc aaattttgat ttggcagaag atgctaaact ccagctttca aaagatactt
               4981 acgatgatga tctggataat ctgttggctc aaattggaga tcaatatgct gatttgtttt
               5041 tggcagctaa gaatctgtca gatgctattc tgctttcaga catcctgaga gtgaatactg
               5101 aaataactaa ggctcccctg tcagcttcaa tgattaaacg ctacgatgaa catcatcaag
               5161 acttgactct tctgaaagcc ctggttagac aacaacttcc agaaaagtat aaagaaatct
               5221 tttttgatca atcaaaaaac ggatatgcag gttatattga tggcggcgca agccaagaag
               5281 aattttataa atttatcaaa ccaattctgg aaaaaatgga tggtactgag gaactgttgg
               5341 tgaaactgaa tagagaagat ttgctgcgca agcaacggac ctttgacaac ggctctattc
               5401 cccatcaaat tcacttgggt gagctgcatg ctattttgag aagacaagaa gacttttatc
               5461 catttctgaa agacaataga gagaagattg aaaaaatctt gacttttagg attccttatt
               5521 atgttggtcc attggccaga ggcaatagta ggtttgcatg gatgactcgg aagtctgaag
               5581 aaacaattac cccatggaat tttgaagaag ttgtcgataa aggtgcttca gctcaatcat
               5641 ttattgaacg catgacaaac tttgataaaa atcttccaaa tgaaaaagtg ctgccaaaac
               5701 atagtttgct ttatgagtat tttaccgttt ataacgaatt gacaaaggtc aaatatgtta
               5761 ctgaaggaat gagaaaacca gcatttcttt caggtgaaca gaagaaagcc attgttgatc
               5821 tgctcttcaa aacaaatagg aaagtgaccg ttaagcaact gaaagaagat tatttcaaaa
               5881 aaatagaatg ttttgatagt gttgaaattt caggagttga agatagattt aatgcttcac
               5941 tgggtacata ccatgatttg ctgaaaatta ttaaagataa agattttttg gataatgaag
               6001 aaaatgaaga catcctggag gatattgttc tgacattgac cctgtttgaa gatagggaga
               6061 tgattgagga aagacttaaa acatacgctc acctctttga tgataaggtg atgaaacagc
               6121 ttaaaagacg cagatatact ggttggggaa ggttgtccag aaaattgatt aatggtatta
               6181 gggataagca atctggcaaa acaatactgg attttttgaa atcagatggt tttgccaatc
               6241 gcaattttat gcagctcatc catgatgata gtttgacatt taaagaagac atccaaaaag
               6301 cacaagtgtc tggacaaggc gatagtctgc atgaacatat tgcaaatctg gctggtagcc
               6361 ctgctattaa aaaaggtatt ctccagactg tgaaagttgt tgatgaattg gtcaaagtga
               6421 tggggcggca taagccagaa aatatcgtta ttgaaatggc aagagaaaat cagacaactc
               6481 aaaagggcca gaaaaattcc agagagagga tgaaaagaat cgaagaaggt atcaaagaac
               6541 tgggaagtca gattcttaaa gagcatcctg ttgaaaatac tcaattgcaa aatgaaaagc
               6601 tctatctcta ttatctccaa aatggaagag atatgtatgt ggaccaagaa ctggatatta
               6661 ataggctgag tgattatgat gtcgatcaca ttgttccaca aagtttcctt aaagacgatt
               6721 caatagacaa taaggtcctg accaggtctg ataaaaatag aggtaaatcc gataacgttc
               6781 caagtgaaga agtggtcaaa aagatgaaaa actattggag acaacttctg aacgccaagc
               6841 tgatcactca aaggaagttt gataatctga ccaaagctga aagaggaggt ttgagtgaac
               6901 ttgataaagc tggttttatc aaacgccaat tggttgaaac tcgccaaatc actaagcatg
               6961 tggcacaaat tttggatagt cgcatgaata ctaaatacga tgaaaatgat aaacttatta
               7021 gagaggttaa agtgattacc ctgaaatcta aactggtttc tgacttcaga aaagatttcc
               7081 aattctataa agtgagagag attaacaatt accatcatgc ccatgatgcc tatctgaatg
               7141 ccgtcgttgg aactgctttg attaagaaat atccaaaact tgaaagcgag tttgtctatg
               7201 gtgattataa agtttatgat gttaggaaaa tgattgctaa gtctgagcaa gaaataggca
               7261 aagcaaccgc aaagtatttc ttttactcta atatcatgaa cttcttcaaa acagaaatta
               7321 cacttgcaaa tggagagatt cgcaaacgcc ctctgatcga aactaatggg gaaactggag
               7381 aaattgtctg ggataaaggg agagattttg ccacagtgcg caaagtgttg tccatgcccc
               7441 aagtcaatat cgtcaagaaa acagaagtgc agacaggcgg attctctaag gagtcaattc
               7501 tgccaaaaag aaattccgac aagctgattg ctaggaaaaa agactgggac ccaaaaaaat
               7561 atggtggttt tgatagtcca accgtggctt attcagtcct ggtggttgct aaggtggaaa
               7621 aagggaaatc caagaagctg aaatccgtta aagagctgct ggggatcaca attatggaaa
               7681 gaagttcctt tgaaaaaaat cccattgact ttctggaagc taaaggatat aaggaagtta
               7741 aaaaagacct gatcattaaa ctgcctaaat atagtctttt tgagctggaa aacggtagga
               7801 aacggatgct ggctagtgcc ggagaactgc aaaaaggaaa tgagctggct ctgccaagca
               7861 aatatgtgaa ttttctgtat ctggctagtc attatgaaaa gttgaagggt agtccagaag
               7921 ataacgaaca aaaacaattg tttgtggagc agcataagca ttatctggat gagattattg
               7981 agcaaatcag tgaattttct aagagagtta ttctggcaga tgccaatctg gataaagttc
               8041 ttagtgcata taacaaacat agagacaaac caataagaga acaagcagaa aatatcattc
               8101 atctgtttac cttgaccaat cttggagcac ccgctgcttt taaatacttt gatacaacaa
               8161 ttgataggaa aagatatacc tctacaaaag aagttctgga tgccactctt atccatcaat
               8221 ccatcactgg tctttatgaa acacgcattg atttgagtca gctgggaggt gaccccaaga
               8281 aaaaacgcaa ggtggaagat cctaagaaaa agcggaaagt ggacacgcgt acgcggccgc
               8341 tcgagcagaa actcatctca gaagaggatc tggcagcaaa tgatatcctg gattacaagg
               8401 atgacgacga taaggtttaa acggccggcc gcggtcatag ctgtttcctg aacagatccc
               8461 gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca
               8521 gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc
               8581 ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca
               8641 acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg
               8701 ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg
               8761 ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagaggcgg
               8821 ggtttcacca tattggccag gctggtctcc aactcctaat ctcaggtgat ctacccacct
               8881 tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttctg
               8941 attttaaaat aactatacca gcaggaggac gtccagacac agcataggct acctggccat
               9001 gcccaaccgg tgggacattt gagttgcttg cttggcactg tcctctcatg cgttgggtcc
               9061 actcagtaga tgcctgttga attgggtacg cggccagcgg cgagcggtat cagctcactc
               9121 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtccgt
               9181 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
               9241 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
               9301 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
               9361 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
               9421 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
               9481 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
               9541 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
               9601 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
               9661 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
               9721 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
               9781 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgac
               9841 gcgtaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
               9901 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
               9961 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
              10021 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
              10081 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
              10141 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
              10201 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
              10261 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
              10321 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
              10381 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
              10441 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
              10501 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
              10561 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
              10621 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
              10681 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
              10741 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
              10801 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
              10861 tcagggttat tgtctcatga tgatattatt ttatcttgtg caatgtaaca tcagagattt
              10921 tgagacacgg gccagagctg ccaggaaaca gctatgacca tgtaatacga ctcactatag
              10981 gggatatcag ctggatggca gttaac
          //
          
          

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