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          PX393

          價格:1000元

          聯系方式:I47-825O-882O

          買家導航

          載體基本信息

          出品公司: Addgene
          載體名稱: PX393 或 PX393 Gluconacetobacter diazotrophicus Cas9
          質粒類型: CRISPR-Cas9載體;基因敲除載體;昆蟲細胞載體
          高拷貝/低拷貝: 高拷貝
          插入位點: 限制性內切酶,多克隆位點
          啟動子: CMV
          載體大小: 10346 bp
          5' 測序引物及序列: CMV Forward:CGCAAATGGGCGGTAGGCGTG
          3' 測序引物及序列: C-CMV-24 (5'-TATTAGGACAAGGCTGGTGGGCAC-3')
          載體標簽: NLS (C ter);HA、NLS (N ter)
          載體抗性: 氨芐青霉素
          篩選標記: Neomycin
          克隆菌株: Stbl3
          宿主細胞(系): 昆蟲細胞
          備注: --
          產品目錄號: #68330
          穩定性: 穩表達
          組成型/誘導型: 組成型
          病毒/非病毒: 桿狀病毒

          載體質粒圖譜和多克隆位點信息

          PX393載體圖譜



          載體簡介

          載體序列

          LOCUS       PX393 Gluconacetobacter diazotrophicus Cas9	10346 bp 	DNA	SYN
          DEFINITION  PX393 Gluconacetobacter diazotrophicus Cas9
          ACCESSION   
          KEYWORDS    
          SOURCE      
            ORGANISM  other sequences; artificial sequences; vectors.
          FEATURES             Location/Qualifiers
               source          1..10346
                               /organism="PX393 Gluconacetobacter diazotrophicus Cas9"
                               /mol_type="other DNA"
               misc_feature    47..1001
                               /label="Ac_5_flank"
               CDS             complement(343..948)
                               /label="ORF frame 3"
                               /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                               VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                               PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                               LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
               promoter        1023..1587
                               /label="CMV_immearly_promoter"
                               /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                               VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                               PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                               LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
               misc_feature    1090..1377
                               /label="CAG_enhancer"
                               /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                               VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                               PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                               LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
               misc_feature    1544..1564
                               /label="CMV_fwd_primer"
                               /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                               VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                               PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                               LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
               promoter        1615..1633
                               /label="T7_promoter"
                               /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                               VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                               PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                               LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
               misc_feature    1633..1656
                               /label="lacO"
                               /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                               VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                               PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                               LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
               misc_feature    1772..1796
                               /label="attB1"
                               /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                               VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                               PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                               LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
               CDS             1822..5037
                               /label="ORF frame 1"
                               /translation="MYPYDVPDYASPKKKRKVEASMGENMIDESLTFGIDLGIGSCGW
                               AVLRRPSAFGRKGVIEGMGSWCFDVPETSKERTPTNQIRRSNRLLRRVIRRRRNRMAA
                               IRRLLHAAGLLPSTDSDALKRPGHDPWELRARGLDKPLKPVEFAVVLGHIAKRRGFKS
                               AAKRKATNISSDDKKMLTALEATRERLGRYRTVGEMFARDPDFASRRRNREGKYDRTT
                               ARDDLEHEVHALFAAQRRLGQGFASPELEEAFTASAFHQRPMQDSERLVGFCPFERTE
                               KRAAKLTPSFERFRLLARLLNLRITTPDGERPLTVDEIALVTRDLGKTAKLSIKRVRT
                               LIGLEDNQRFTTIRPEDEDRDIVARTGGAMTGTATLRKALGEALWTDMQERPEQLDAI
                               VQVLSFFEANETITEKLREIGLTLAVLDVLLTALDAGVFAKFKGAAHISTKAARNLLP
                               HLEQGRRYDEACTMAGYDHAASRLSHHGQIVAKTQFNALVTEIGESIANPIARKALIE
                               GLKQIWAMRNHWGLPGSIHVELARDVGNSIEKRREIEKHIEKNTALRARERREVHDLL
                               DLEDVNGDTLLRYRLWKEQGGKCLYTGKAIHIRQIAATDNSVQVDHILPWSRFGDDSF
                               NNKTLCLASANQQKKRSTPYEWLSGQTGDAWNAFVQRIETNKELRGFKKRNYLLKNAK
                               EAEEKFRSRNLNDTRYAARLFAEAVKLLYAFGERQEKGGNRRVFTRPGALTAALRQAW
                               GVESLKKQDGKRINDDRHHALDALTVAAVDEAEIQRLTKSFHEWEQQGLGRPLRRVEP
                               PWESFRADVEATYPEVFVARPERRRARGEGHAATIRQVKERECTPIVFERKAVSSLKE
                               ADLERIKDGERNEAIVEAIRSWIATGRPADAPPRSPRGDIITKIRLATTIKAAVPVRG
                               GTAGRGEMVRADVFSKPNRRGKDEWYLVPVYPHQIMNRKAWPKPPMRSIVANKDEDEW
                               TEVGPEHQFRFSLYPRSNIEIIRPSGEVIEGYFVGLHRNTGALTISAHNDPKSIHSGI
                               GTKTLLAISKYQVDRFGRKSPVRKEVRTWHGEACISPTPPG*"
               misc_feature    1825..1851
                               /label="HA"
                               /translation="MYPYDVPDYASPKKKRKVEASMGENMIDESLTFGIDLGIGSCGW
                               AVLRRPSAFGRKGVIEGMGSWCFDVPETSKERTPTNQIRRSNRLLRRVIRRRRNRMAA
                               IRRLLHAAGLLPSTDSDALKRPGHDPWELRARGLDKPLKPVEFAVVLGHIAKRRGFKS
                               AAKRKATNISSDDKKMLTALEATRERLGRYRTVGEMFARDPDFASRRRNREGKYDRTT
                               ARDDLEHEVHALFAAQRRLGQGFASPELEEAFTASAFHQRPMQDSERLVGFCPFERTE
                               KRAAKLTPSFERFRLLARLLNLRITTPDGERPLTVDEIALVTRDLGKTAKLSIKRVRT
                               LIGLEDNQRFTTIRPEDEDRDIVARTGGAMTGTATLRKALGEALWTDMQERPEQLDAI
                               VQVLSFFEANETITEKLREIGLTLAVLDVLLTALDAGVFAKFKGAAHISTKAARNLLP
                               HLEQGRRYDEACTMAGYDHAASRLSHHGQIVAKTQFNALVTEIGESIANPIARKALIE
                               GLKQIWAMRNHWGLPGSIHVELARDVGNSIEKRREIEKHIEKNTALRARERREVHDLL
                               DLEDVNGDTLLRYRLWKEQGGKCLYTGKAIHIRQIAATDNSVQVDHILPWSRFGDDSF
                               NNKTLCLASANQQKKRSTPYEWLSGQTGDAWNAFVQRIETNKELRGFKKRNYLLKNAK
                               EAEEKFRSRNLNDTRYAARLFAEAVKLLYAFGERQEKGGNRRVFTRPGALTAALRQAW
                               GVESLKKQDGKRINDDRHHALDALTVAAVDEAEIQRLTKSFHEWEQQGLGRPLRRVEP
                               PWESFRADVEATYPEVFVARPERRRARGEGHAATIRQVKERECTPIVFERKAVSSLKE
                               ADLERIKDGERNEAIVEAIRSWIATGRPADAPPRSPRGDIITKIRLATTIKAAVPVRG
                               GTAGRGEMVRADVFSKPNRRGKDEWYLVPVYPHQIMNRKAWPKPPMRSIVANKDEDEW
                               TEVGPEHQFRFSLYPRSNIEIIRPSGEVIEGYFVGLHRNTGALTISAHNDPKSIHSGI
                               GTKTLLAISKYQVDRFGRKSPVRKEVRTWHGEACISPTPPG*"
               CDS             complement(1845..2327)
                               /label="ORF frame 1"
                               /translation="MFVAFLFAADLNPLRFAMWPRTTANSTGFSGLSRPRALSSQGSC
                               PGRFRASLSVDGSRPAACSSRRIAAIRFLLRRITRLSSLLDLRIWLVGVRSLDVSGTS
                               KHQEPIPSITPFRPKALGRLSTAHPQLPIPRSMPKVRLSSIIFSPMDASTLRFFFGEA
                               *"
               misc_feature    1855..1875
                               /label="SV40 NLS"
                               /translation="MFVAFLFAADLNPLRFAMWPRTTANSTGFSGLSRPRALSSQGSC
                               PGRFRASLSVDGSRPAACSSRRIAAIRFLLRRITRLSSLLDLRIWLVGVRSLDVSGTS
                               KHQEPIPSITPFRPKALGRLSTAHPQLPIPRSMPKVRLSSIIFSPMDASTLRFFFGEA
                               *"
               misc_feature    1861..1875
                               /label="NLS"
                               /translation="MFVAFLFAADLNPLRFAMWPRTTANSTGFSGLSRPRALSSQGSC
                               PGRFRASLSVDGSRPAACSSRRIAAIRFLLRRITRLSSLLDLRIWLVGVRSLDVSGTS
                               KHQEPIPSITPFRPKALGRLSTAHPQLPIPRSMPKVRLSSIIFSPMDASTLRFFFGEA
                               *"
               CDS             complement(2578..3402)
                               /label="ORF frame 3"
                               /translation="MDTSGQPPVVSHGPNLFQPLDQCLPSDWIGNGFADLSDQGVELC
                               LCHDLAMVRQAGGCVVIPCHCAGLIVAPALLQMWQQIPSGFGADVCSAFKLGEHSGIQ
                               CGQQDVQHGQSQANFPQLLSDCFVGLKEAQDLHDSVQLFRALLHISPQGLSQCLPQGG
                               SPCHCPACPSHNVSIFVLGADSCEPLIVLQSNQSSHPLDTQFCGLAQVPGDQSNFIHC
                               QGPLSVWGSDSQVQQPGQQSKALERGCQFGCPLFGSLKRAESHQALAVLHGPLMKC*"
               CDS             complement(4251..4934)
                               /label="ORF frame 1"
                               /translation="MASSVLVPMPEWILLGSLCALMVRAPVLRCRPTKYPSITSPDGL
                               MISILDLGYRLNRNWCSGPTSVHSSSSLLATIERIGGLGQAFLFMIWCGYTGTRYHSS
                               FPLRFGLLNTSARTISPLPAVPPRTGTAALMVVASLILVMMSPRGERGGASAGRPVAI
                               QLLMASTIASLRSPSLIRSRSASFRLETAFLSNTIGVHSLSFTCRMVAAWPSPLARRL
                               SGRATNTSG*"
               misc_feature    complement(5070..5087)
                               /label="Sp6_primer"
                               /translation="MASSVLVPMPEWILLGSLCALMVRAPVLRCRPTKYPSITSPDGL
                               MISILDLGYRLNRNWCSGPTSVHSSSSLLATIERIGGLGQAFLFMIWCGYTGTRYHSS
                               FPLRFGLLNTSARTISPLPAVPPRTGTAALMVVASLILVMMSPRGERGGASAGRPVAI
                               QLLMASTIASLRSPSLIRSRSASFRLETAFLSNTIGVHSLSFTCRMVAAWPSPLARRL
                               SGRATNTSG*"
               misc_feature    5092..5116
                               /label="attB2"
                               /translation="MASSVLVPMPEWILLGSLCALMVRAPVLRCRPTKYPSITSPDGL
                               MISILDLGYRLNRNWCSGPTSVHSSSSLLATIERIGGLGQAFLFMIWCGYTGTRYHSS
                               FPLRFGLLNTSARTISPLPAVPPRTGTAALMVVASLILVMMSPRGERGGASAGRPVAI
                               QLLMASTIASLRSPSLIRSRSASFRLETAFLSNTIGVHSLSFTCRMVAAWPSPLARRL
                               SGRATNTSG*"
               terminator      5128..5594
                               /label="hGH_PA_terminator"
                               /translation="MASSVLVPMPEWILLGSLCALMVRAPVLRCRPTKYPSITSPDGL
                               MISILDLGYRLNRNWCSGPTSVHSSSSLLATIERIGGLGQAFLFMIWCGYTGTRYHSS
                               FPLRFGLLNTSARTISPLPAVPPRTGTAALMVVASLILVMMSPRGERGGASAGRPVAI
                               QLLMASTIASLRSPSLIRSRSASFRLETAFLSNTIGVHSLSFTCRMVAAWPSPLARRL
                               SGRATNTSG*"
               terminator      5600..5635
                               /label="T7_terminator"
                               /translation="MASSVLVPMPEWILLGSLCALMVRAPVLRCRPTKYPSITSPDGL
                               MISILDLGYRLNRNWCSGPTSVHSSSSLLATIERIGGLGQAFLFMIWCGYTGTRYHSS
                               FPLRFGLLNTSARTISPLPAVPPRTGTAALMVVASLILVMMSPRGERGGASAGRPVAI
                               QLLMASTIASLRSPSLIRSRSASFRLETAFLSNTIGVHSLSFTCRMVAAWPSPLARRL
                               SGRATNTSG*"
               rep_origin      5752..6058
                               /label="f1_origin"
                               /translation="MASSVLVPMPEWILLGSLCALMVRAPVLRCRPTKYPSITSPDGL
                               MISILDLGYRLNRNWCSGPTSVHSSSSLLATIERIGGLGQAFLFMIWCGYTGTRYHSS
                               FPLRFGLLNTSARTISPLPAVPPRTGTAALMVVASLILVMMSPRGERGGASAGRPVAI
                               QLLMASTIASLRSPSLIRSRSASFRLETAFLSNTIGVHSLSFTCRMVAAWPSPLARRL
                               SGRATNTSG*"
               misc_feature    complement(6172..6192)
                               /label="pBABE_3_primer"
                               /translation="MASSVLVPMPEWILLGSLCALMVRAPVLRCRPTKYPSITSPDGL
                               MISILDLGYRLNRNWCSGPTSVHSSSSLLATIERIGGLGQAFLFMIWCGYTGTRYHSS
                               FPLRFGLLNTSARTISPLPAVPPRTGTAALMVVASLILVMMSPRGERGGASAGRPVAI
                               QLLMASTIASLRSPSLIRSRSASFRLETAFLSNTIGVHSLSFTCRMVAAWPSPLARRL
                               SGRATNTSG*"
               misc_feature    complement(6178..6393)
                               /label="SV40_enhancer"
                               /translation="MASSVLVPMPEWILLGSLCALMVRAPVLRCRPTKYPSITSPDGL
                               MISILDLGYRLNRNWCSGPTSVHSSSSLLATIERIGGLGQAFLFMIWCGYTGTRYHSS
                               FPLRFGLLNTSARTISPLPAVPPRTGTAALMVVASLILVMMSPRGERGGASAGRPVAI
                               QLLMASTIASLRSPSLIRSRSASFRLETAFLSNTIGVHSLSFTCRMVAAWPSPLARRL
                               SGRATNTSG*"
               promoter        6190..6458
                               /label="SV40_promoter"
                               /translation="MASSVLVPMPEWILLGSLCALMVRAPVLRCRPTKYPSITSPDGL
                               MISILDLGYRLNRNWCSGPTSVHSSSSLLATIERIGGLGQAFLFMIWCGYTGTRYHSS
                               FPLRFGLLNTSARTISPLPAVPPRTGTAALMVVASLILVMMSPRGERGGASAGRPVAI
                               QLLMASTIASLRSPSLIRSRSASFRLETAFLSNTIGVHSLSFTCRMVAAWPSPLARRL
                               SGRATNTSG*"
               rep_origin      6357..6434
                               /label="SV40_origin"
                               /translation="MASSVLVPMPEWILLGSLCALMVRAPVLRCRPTKYPSITSPDGL
                               MISILDLGYRLNRNWCSGPTSVHSSSSLLATIERIGGLGQAFLFMIWCGYTGTRYHSS
                               FPLRFGLLNTSARTISPLPAVPPRTGTAALMVVASLILVMMSPRGERGGASAGRPVAI
                               QLLMASTIASLRSPSLIRSRSASFRLETAFLSNTIGVHSLSFTCRMVAAWPSPLARRL
                               SGRATNTSG*"
               misc_feature    6419..6438
                               /label="SV40pro_F_primer"
                               /translation="MASSVLVPMPEWILLGSLCALMVRAPVLRCRPTKYPSITSPDGL
                               MISILDLGYRLNRNWCSGPTSVHSSSSLLATIERIGGLGQAFLFMIWCGYTGTRYHSS
                               FPLRFGLLNTSARTISPLPAVPPRTGTAALMVVASLILVMMSPRGERGGASAGRPVAI
                               QLLMASTIASLRSPSLIRSRSASFRLETAFLSNTIGVHSLSFTCRMVAAWPSPLARRL
                               SGRATNTSG*"
               CDS             6573..7367
                               /label="ORF frame 3"
                               /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                               PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                               LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                               EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                               QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
               gene            6576..7364
                               /label="NeoR/KanR"
                               /gene="NeoR/KanR"
                               /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                               PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                               LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                               EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                               QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
               CDS             complement(6882..7418)
                               /label="ORF frame 1"
                               /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                               VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                               VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                               PPHCISHDGYFLGRSKVR*"
               terminator      7544..7663
                               /label="SV40_PA_terminator"
                               /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                               VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                               VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                               PPHCISHDGYFLGRSKVR*"
               misc_feature    7632..7651
                               /label="EBV_rev_primer"
                               /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                               VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                               VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                               PPHCISHDGYFLGRSKVR*"
               terminator      7854..7889
                               /label="T7_terminator"
                               /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                               VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                               VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                               PPHCISHDGYFLGRSKVR*"
               misc_feature    complement(7912..7929)
                               /label="Baculovirs_rev_primer"
                               /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                               VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                               VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                               PPHCISHDGYFLGRSKVR*"
               misc_feature    7919..7996
                               /label="3_AcPH"
                               /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                               VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                               VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                               PPHCISHDGYFLGRSKVR*"
               CDS             complement(8026..8643)
                               /label="ORF frame 3"
                               /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                               NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                               QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                               KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
               misc_feature    8623..8656
                               /label="loxP"
                               /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                               NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                               QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                               KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
               gene            complement(9424..10284)
                               /label="Ampicillin"
                               /gene="Ampicillin"
                               /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                               NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                               QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                               KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
               CDS             complement(9424..10284)
                               /label="ORF frame 3"
                               /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                               IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                               YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                               DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                               LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                               EIGASLIKHW*"
          ORIGIN
              1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
             61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
            121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
            181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
            241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
            301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
            361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
            421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
            481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
            541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
            601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
            661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
            721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
            781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
            841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
            901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
            961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccc
           1021 tcgagtagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
           1081 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
           1141 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
           1201 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
           1261 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
           1321 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
           1381 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
           1441 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
           1501 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
           1561 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtgg atcttaatac
           1621 gactcactat aggggaattg tgagcggata acaattccgg gacctttaat tcaacccaac
           1681 acaatatatt atagttaaat aagaattatt atcaaatcat ttgtatatta attaaaatac
           1741 tatactgtaa attacatttt atttacaatc aacaagtttg tacaaaaaag caggctaagg
           1801 agatatacca ccggtgccac catgtaccca tacgatgttc cagattacgc ttcgccgaag
           1861 aaaaagcgca aggtcgaagc gtccatggga gagaatatga ttgacgagag tctgaccttc
           1921 ggcattgacc tggggattgg gagttgtggg tgggcagtgc tgaggcggcc aagcgccttc
           1981 ggacggaagg gcgtgatcga gggaatgggc tcctggtgct ttgacgtccc cgagacatct
           2041 aaagaacgga ctcctaccaa ccagatccgg agatccaata gactgctgag gcgcgtgatc
           2101 cgacggagaa ggaaccggat ggccgctatt cgccgactgc tgcacgcagc aggcctgctg
           2161 ccatcaaccg acagcgatgc cctgaagcgg cccggacatg atccttggga actgagggca
           2221 cgaggcctgg acaagccact gaaacccgtg gagttcgctg tggtcctggg ccacatcgca
           2281 aagcggagag ggtttaaatc cgctgcaaag agaaaagcca caaacattag ctccgacgat
           2341 aagaaaatgc tgacagccct ggaggctact cgagaacggc tggggagata caggaccgtg
           2401 ggagaaatgt tcgccaggga ccctgatttt gcttctaggc gccgaaatcg cgagggcaag
           2461 tatgatagga ccacagctcg cgacgatctg gagcacgaag tgcacgccct gttcgcagct
           2521 cagcggagac tgggacaggg atttgccagt ccagaactgg aggaggcctt caccgcttca
           2581 gcatttcatc agaggcccat gcaggacagc gagcgcctgg tgggattctg cccttttgag
           2641 cgaaccgaaa agcgggcagc caaactgaca ccctctttcg agcgctttcg actgctggcc
           2701 cggctgctga acctgagaat cactacccca gacggagagc ggcccctgac agtggatgaa
           2761 attgctctgg tcacccggga cctgggcaag accgcaaaac tgagtatcaa gcgggtgaga
           2821 actctgattg gactggagga caatcagagg ttcacaacta tccgccccga ggacgaagat
           2881 cgagacattg tggctcggac aggcggggca atgacaggga ctgccaccct gaggaaggca
           2941 ctgggagagg ccctgtggac tgatatgcag gagcgccctg aacagctgga cgctatcgtg
           3001 caggtcctga gcttctttga ggccaacgaa acaatcactg agaagctgag ggaaattggc
           3061 ctgactctgg ccgtgctgga cgtcctgctg accgcactgg atgccggagt gttcgccaag
           3121 tttaaaggcg ctgcacacat cagcaccaaa gccgctagga atctgctgcc acatctggag
           3181 cagggcaggc gctacgatga ggcctgcaca atggcagggt atgaccacgc agcctcccgc
           3241 ctgtctcacc atggccagat cgtggcaaag acacagttca acgccctggt cactgagatc
           3301 ggcgaatcca ttgccaatcc aatcgctcgg aaggcactga tcgaggggct gaaacagatt
           3361 tgggccatga gaaaccactg ggggctgccc ggaagtatcc atgtggagct ggcccgggat
           3421 gtcggcaact caattgaaaa gcgacgggag attgaaaagc acatcgagaa aaatactgcc
           3481 ctgagggctc gcgagagaag ggaggtgcat gatctgctgg acctggaaga tgtcaatggc
           3541 gacaccctgc tgcgataccg gctgtggaag gagcagggag gcaaatgcct gtatacaggg
           3601 aaggccatcc acattcggca gatcgctgca actgacaact ccgtgcaggt cgatcatatt
           3661 ctgccttgga gccggttcgg cgacgatagt tttaacaaca agaccctgtg tctggcctct
           3721 gctaatcagc agaagaaaag gtcaacacca tacgagtggc tgagcggcca gactggggat
           3781 gcatggaacg ccttcgtgca gcgcatcgag acaaataagg aactgagagg gtttaagaaa
           3841 aggaactatc tgctgaagaa tgctaaagag gcagaggaaa aattcagaag caggaacctg
           3901 aatgacacca gatacgccgc taggctgttc gcagaggccg tgaagctgct gtatgccttt
           3961 ggggagagac aggaaaaagg gggaaaccgc cgagtgttta ctcggcctgg agcactgacc
           4021 gcagcactga gacaggcttg gggagtggag agcctgaaga aacaggatgg gaagcgcatc
           4081 aatgacgatc gacaccatgc cctggatgct ctgaccgtgg ctgcagtcga cgaggccgaa
           4141 attcagaggc tgacaaaatc attccacgag tgggaacagc agggcctggg gcggcctctg
           4201 cggagagtgg agccaccttg ggagagcttc cgggcagacg tcgaggctac ctaccctgaa
           4261 gtgtttgtcg cacggccaga gaggcgccga gcaagaggag aaggccatgc cgctaccatc
           4321 cggcaggtga aggagagaga atgcacacca attgtgtttg agagaaaggc tgtctctagt
           4381 ctgaaagagg cagacctgga acgaatcaaa gatggcgagc gcaacgaagc aattgtggag
           4441 gccatcagga gctggattgc cactggacgc ccagctgatg caccaccacg ctccccccga
           4501 ggcgacatca ttaccaagat caggctggcc accaccatca aggcagccgt gcctgtccgc
           4561 ggagggaccg caggaagggg agaaatggtg cgcgcagatg tgttcagcaa gccaaaccgg
           4621 agagggaaag acgagtggta tctggtgccc gtgtatccac accagatcat gaacaggaag
           4681 gcttggccca aacctccaat gcgctcaatt gtggccaata aggatgagga cgaatggacc
           4741 gaagtgggac ctgaacacca gttccggttt agcctgtacc ctagatccaa tatcgagatc
           4801 attaggccat ctggagaagt gatcgaagga tatttcgtcg gcctgcatcg caacactggc
           4861 gctctgacca tcagtgcaca caatgatccc aagagtatcc attcaggcat tgggaccaag
           4921 acactgctgg ccatttccaa ataccaggtg gacagattcg gcagaaagtc tccagtgcgc
           4981 aaagaggtcc gaacttggca cggggaagcc tgtatctctc ccaccccccc tggataagaa
           5041 ttcggtaccg agctcaagct tgcggccgcc tatagtgtca cctaaatcgt aacccagctt
           5101 tcttgtacaa agtggtcggg tggcatccct gtgacccctc cccagtgcct ctcctggccc
           5161 tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt tgcatcattt
           5221 tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta tggagcaagg
           5281 ggcaagttgg gatgacaacc tgtagggcct gcggggtcta ttgggaacca agctggagtg
           5341 cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg attctcctgc
           5401 ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc taatttttgt
           5461 ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac tcctaatctc
           5521 aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga accactgctc
           5581 ccttccctgt ccttctagca taaccccttg gggcctctaa acgggtcttg aggggttttt
           5641 tgtctagaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
           5701 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
           5761 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
           5821 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
           5881 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
           5941 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
           6001 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
           6061 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
           6121 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
           6181 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
           6241 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
           6301 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
           6361 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
           6421 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
           6481 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
           6541 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
           6601 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
           6661 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
           6721 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
           6781 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
           6841 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
           6901 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
           6961 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
           7021 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
           7081 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
           7141 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
           7201 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
           7261 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
           7321 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
           7381 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
           7441 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
           7501 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
           7561 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
           7621 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gttcatgaag
           7681 ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt
           7741 tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat
           7801 cagaatgagt atttggttta gagtttggca acatatgccc atatgtaact agcataaccc
           7861 cttggggcct ctaaacgggt cttgaggggt tttttgctga aagcatgcgg aggaaattct
           7921 ccttgaagtt tccctggtgt tcaaagtaaa ggagtttgca ccagacgcac ctctgttcac
           7981 tggtccggcg tattaaaaca cgatacattg ttattagtac atttattaag cgctagattc
           8041 tgtgcgttgt tgatttacag acaattgttg tacgtatttt aataattcat taaatttata
           8101 atctttaggg tggtatgtta gagcgaaaat caaatgattt tcagcgtctt tatatctgaa
           8161 tttaaatatt aaatcctcaa tagatttgta aaataggttt cgattagttt caaacaaggg
           8221 ttgtttttcc gaaccgatgg ctggactatc taatggattt tcgctcaacg ccacaaaact
           8281 tgccaaatct tgtagcagca atctagcttt gtcgatattc gtttgtgttt tgttttgtaa
           8341 taaaggttcg acgtcgttca aaatattatg cgcttttgta tttctttcat cactgtcgtt
           8401 agtgtacaat tgactcgacg taaacacgtt aaatagagct tggacatatt taacatcggg
           8461 cgtgttagct ttattaggcc gattatcgtc gtcgtcccaa ccctcgtcgt tagaagttgc
           8521 ttccgaagac gattttgcca tagccacacg acgcctatta attgtgtcgg ctaacacgtc
           8581 cgcgatcaaa tttgtagttg agctttttgg aattgcgatc gcataacttc gtatagcata
           8641 cattatacga agttataagc tcggaacgct gcgctcggtc gttcggctgc ggcgagcggt
           8701 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
           8761 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
           8821 gtttttccat aggctccgcc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
           8881 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
           8941 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
           9001 agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
           9061 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
           9121 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
           9181 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
           9241 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
           9301 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
           9361 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
           9421 ttgttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
           9481 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
           9541 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
           9601 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
           9661 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
           9721 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
           9781 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
           9841 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
           9901 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
           9961 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
          10021 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
          10081 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
          10141 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
          10201 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
          10261 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
          10321 gggttattgt ctcatgtccg cgcgtt
          //
          

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