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          PX389

          價格:1000元

          聯系方式:I47-825O-882O

          買家導航

          載體基本信息

          出品公司: Addgene
          載體名稱: PX389 或 PX389 Fluviicola taffensis Cas9
          質粒類型: CRISPR-Cas9載體;基因敲除載體;昆蟲細胞載體
          高拷貝/低拷貝: 高拷貝
          插入位點: 限制性內切酶,多克隆位點
          啟動子: CMV
          載體大小: 11570 bp
          5' 測序引物及序列: CMV Forward:CGCAAATGGGCGGTAGGCGTG
          3' 測序引物及序列: C-CMV-24 (5'-TATTAGGACAAGGCTGGTGGGCAC-3')
          載體標簽: NLS (C ter);HA、NLS (N ter)
          載體抗性: 氨芐青霉素
          篩選標記: Neomycin
          克隆菌株: Stbl3
          宿主細胞(系): 昆蟲細胞
          備注: --
          產品目錄號: #68326
          穩定性: 穩表達
          組成型/誘導型: 組成型
          病毒/非病毒: 桿狀病毒

          載體質粒圖譜和多克隆位點信息

          PX389載體圖譜



          載體簡介

          載體序列

          LOCUS       PX389 Fluviicola taffensis Cas9	11570 bp 	DNA	SYN
          DEFINITION  PX389 Fluviicola taffensis Cas9
          ACCESSION   
          KEYWORDS    
          SOURCE      
            ORGANISM  other sequences; artificial sequences; vectors.
          FEATURES             Location/Qualifiers
               source          1..11570
                               /organism="PX389 Fluviicola taffensis Cas9"
                               /mol_type="other DNA"
               misc_feature    47..1001
                               /label="Ac_5_flank"
               CDS             complement(343..948)
                               /label="ORF frame 3"
                               /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                               VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                               PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                               LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
               promoter        1023..1587
                               /label="CMV_immearly_promoter"
                               /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                               VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                               PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                               LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
               misc_feature    1090..1377
                               /label="CAG_enhancer"
                               /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                               VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                               PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                               LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
               misc_feature    1544..1564
                               /label="CMV_fwd_primer"
                               /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                               VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                               PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                               LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
               promoter        1615..1633
                               /label="T7_promoter"
                               /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                               VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                               PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                               LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
               misc_feature    1633..1656
                               /label="lacO"
                               /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                               VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                               PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                               LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
               misc_feature    1772..1796
                               /label="attB1"
                               /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                               VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                               PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                               LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
               CDS             complement(1795..2502)
                               /label="ORF frame 3"
                               /translation="MKLHQLIWCLFLFLASQLIVSPFLIEVQQDPCQFLLLNFLRQSF
                               LSQIVDCPVMGNFLAVAHQLWLVDFKVDQHFLVALLKQEHVISIETDFWLRFRLELAE
                               PLLKVNLVGIVFWQEANDIQHSMQPLAPQQSSVDQSPDSPQSSSLSCLTDRVAFSELV
                               LNILCHRNDPAAHAQDLPLFVIEVLVNQCPSNAVSPQIQSQNSLCHGRFDLALFLRRS
                               VIWNIVWVHGGTGGISP*"
               CDS             1822..6261
                               /label="ORF frame 1"
                               /translation="MYPYDVPDYASPKKKRKVEASMTKTILGLDLGTNSIGWALINQD
                               FDNKKGEILGMGSRIIPMTQDIKDEFGKGNSISQTAERTRLRGVRRLIHRTLLRRERL
                               HRVLNIIGFLPEHYANQIDFEKRFGKFKPESEPKISFDGNDVFLFEKSYQEMLVDFKI
                               HQPQLVSNGKKIPHDWTIYYLRKKALSKKIEKEELAWILLNFNQKRGYYQLRGEEEEE
                               TPNKLVEFHSLKIVEVNADEPQKGKPEIWYSLVLENGWVYRRASKTPLFDWKDKIREF
                               IVTTEINNDGTVKTDKEGTEKRSFRAPKPEDWTLQKKKTEFELSKSGNEVGAFIYESI
                               LQKPNQKIRGKLISTIERKFYKEELKTILKTQLFFHKELKDEKLYNACIEELYKNNEA
                               HRSLLSNKGFEHLFINDILFYQRPLRSKKSQISNCPLEKRTYKKEGIEITEGIKVISK
                               SNPIYQEFRLWQWISNLSLYCIEPTETNVTSTFLNSIEDYENLFEFLNNRKEIEQKHL
                               LKYLLENQGFKGKLLTNELEKFRWNFVADKKYPCNETGSLLHTRLSKVKNISPDFLTK
                               EIEHQLWHIIYSVTDKIEYEQALKTFARKNNLDVDSFFEHFKKIPPFESTYGAYSEKA
                               IKKLLPLIRLGKYWNWEAIDSISKDRISKILSGEYDENIKNRVREKAVHLTSENNFQG
                               LQEWLAKYIVYDRHSEGNDLGKWTSVSDLETYLKEFKQHSLRNPIVEQVITETLRVVK
                               DIWIKHGKGTENFFDEIHVELGREMKNNSEDRKRLTNTITENENTNLRIKALLMEMMN
                               DNDVENVRPYSPSQQEILKIYEDGALNSNIELDDEIVKISKKAEPTKSELQRYKLWLE
                               QKYRSPYTGQVIPLNKLFTSEYEIEHVVPQSRFFDDSFSNKVICESAVNKRKDNQLGL
                               QFIKNHSGEKVELGFGKVVQVFTEEQYLDFVKEHYSKNRSKHNKLLLEEIPEKMIERQ
                               LNDTRYISKFVSSILSNIVRSEKDDDGLNSKNILPGNGKITTELKRDWGLNDVWNDLI
                               LPRFERMNTITNSDLFTTYNDKYQKHLPTVPFEYSKGFQKKRIDHRHHAMDALVIACA
                               TRDHLNLMNNQSAKSELKRYDLQNKLRKKEPYFNQKENKQKEAFKDFIKPWGTFTEDS
                               KNALEKIIISFKQNLRVINKATNSYESYKDEDGNLRIGKDGKPEKGLIKQKGLNWAIR
                               KPLHKDTVSGQINLSRIKLPNGKILTATRKNLDTSFDLKTIENSITDTGIQKILKNYL
                               LSKESPELAFSPEGLEEMNNEIEKYNNGKFHHPINKARIYELGSKFNVGQTGNRRDKF
                               VEAAKGTNLFFAIYQDENKNRSYETIPLNEVIEHQKWRASLPKEEQEKIPLVPVNKLK
                               GTFIFSLSPNDLVYVPSNDELERSASIDFSKLKKEQINRLYKMVSSSGSQCFFVKSEV
                               ATSVVNKMEYSSLNKMEKSIDNLMVKEICIKLKIDRLGNISKA*"
               misc_feature    1825..1851
                               /label="HA"
                               /translation="MYPYDVPDYASPKKKRKVEASMTKTILGLDLGTNSIGWALINQD
                               FDNKKGEILGMGSRIIPMTQDIKDEFGKGNSISQTAERTRLRGVRRLIHRTLLRRERL
                               HRVLNIIGFLPEHYANQIDFEKRFGKFKPESEPKISFDGNDVFLFEKSYQEMLVDFKI
                               HQPQLVSNGKKIPHDWTIYYLRKKALSKKIEKEELAWILLNFNQKRGYYQLRGEEEEE
                               TPNKLVEFHSLKIVEVNADEPQKGKPEIWYSLVLENGWVYRRASKTPLFDWKDKIREF
                               IVTTEINNDGTVKTDKEGTEKRSFRAPKPEDWTLQKKKTEFELSKSGNEVGAFIYESI
                               LQKPNQKIRGKLISTIERKFYKEELKTILKTQLFFHKELKDEKLYNACIEELYKNNEA
                               HRSLLSNKGFEHLFINDILFYQRPLRSKKSQISNCPLEKRTYKKEGIEITEGIKVISK
                               SNPIYQEFRLWQWISNLSLYCIEPTETNVTSTFLNSIEDYENLFEFLNNRKEIEQKHL
                               LKYLLENQGFKGKLLTNELEKFRWNFVADKKYPCNETGSLLHTRLSKVKNISPDFLTK
                               EIEHQLWHIIYSVTDKIEYEQALKTFARKNNLDVDSFFEHFKKIPPFESTYGAYSEKA
                               IKKLLPLIRLGKYWNWEAIDSISKDRISKILSGEYDENIKNRVREKAVHLTSENNFQG
                               LQEWLAKYIVYDRHSEGNDLGKWTSVSDLETYLKEFKQHSLRNPIVEQVITETLRVVK
                               DIWIKHGKGTENFFDEIHVELGREMKNNSEDRKRLTNTITENENTNLRIKALLMEMMN
                               DNDVENVRPYSPSQQEILKIYEDGALNSNIELDDEIVKISKKAEPTKSELQRYKLWLE
                               QKYRSPYTGQVIPLNKLFTSEYEIEHVVPQSRFFDDSFSNKVICESAVNKRKDNQLGL
                               QFIKNHSGEKVELGFGKVVQVFTEEQYLDFVKEHYSKNRSKHNKLLLEEIPEKMIERQ
                               LNDTRYISKFVSSILSNIVRSEKDDDGLNSKNILPGNGKITTELKRDWGLNDVWNDLI
                               LPRFERMNTITNSDLFTTYNDKYQKHLPTVPFEYSKGFQKKRIDHRHHAMDALVIACA
                               TRDHLNLMNNQSAKSELKRYDLQNKLRKKEPYFNQKENKQKEAFKDFIKPWGTFTEDS
                               KNALEKIIISFKQNLRVINKATNSYESYKDEDGNLRIGKDGKPEKGLIKQKGLNWAIR
                               KPLHKDTVSGQINLSRIKLPNGKILTATRKNLDTSFDLKTIENSITDTGIQKILKNYL
                               LSKESPELAFSPEGLEEMNNEIEKYNNGKFHHPINKARIYELGSKFNVGQTGNRRDKF
                               VEAAKGTNLFFAIYQDENKNRSYETIPLNEVIEHQKWRASLPKEEQEKIPLVPVNKLK
                               GTFIFSLSPNDLVYVPSNDELERSASIDFSKLKKEQINRLYKMVSSSGSQCFFVKSEV
                               ATSVVNKMEYSSLNKMEKSIDNLMVKEICIKLKIDRLGNISKA*"
               misc_feature    1855..1875
                               /label="SV40 NLS"
                               /translation="MYPYDVPDYASPKKKRKVEASMTKTILGLDLGTNSIGWALINQD
                               FDNKKGEILGMGSRIIPMTQDIKDEFGKGNSISQTAERTRLRGVRRLIHRTLLRRERL
                               HRVLNIIGFLPEHYANQIDFEKRFGKFKPESEPKISFDGNDVFLFEKSYQEMLVDFKI
                               HQPQLVSNGKKIPHDWTIYYLRKKALSKKIEKEELAWILLNFNQKRGYYQLRGEEEEE
                               TPNKLVEFHSLKIVEVNADEPQKGKPEIWYSLVLENGWVYRRASKTPLFDWKDKIREF
                               IVTTEINNDGTVKTDKEGTEKRSFRAPKPEDWTLQKKKTEFELSKSGNEVGAFIYESI
                               LQKPNQKIRGKLISTIERKFYKEELKTILKTQLFFHKELKDEKLYNACIEELYKNNEA
                               HRSLLSNKGFEHLFINDILFYQRPLRSKKSQISNCPLEKRTYKKEGIEITEGIKVISK
                               SNPIYQEFRLWQWISNLSLYCIEPTETNVTSTFLNSIEDYENLFEFLNNRKEIEQKHL
                               LKYLLENQGFKGKLLTNELEKFRWNFVADKKYPCNETGSLLHTRLSKVKNISPDFLTK
                               EIEHQLWHIIYSVTDKIEYEQALKTFARKNNLDVDSFFEHFKKIPPFESTYGAYSEKA
                               IKKLLPLIRLGKYWNWEAIDSISKDRISKILSGEYDENIKNRVREKAVHLTSENNFQG
                               LQEWLAKYIVYDRHSEGNDLGKWTSVSDLETYLKEFKQHSLRNPIVEQVITETLRVVK
                               DIWIKHGKGTENFFDEIHVELGREMKNNSEDRKRLTNTITENENTNLRIKALLMEMMN
                               DNDVENVRPYSPSQQEILKIYEDGALNSNIELDDEIVKISKKAEPTKSELQRYKLWLE
                               QKYRSPYTGQVIPLNKLFTSEYEIEHVVPQSRFFDDSFSNKVICESAVNKRKDNQLGL
                               QFIKNHSGEKVELGFGKVVQVFTEEQYLDFVKEHYSKNRSKHNKLLLEEIPEKMIERQ
                               LNDTRYISKFVSSILSNIVRSEKDDDGLNSKNILPGNGKITTELKRDWGLNDVWNDLI
                               LPRFERMNTITNSDLFTTYNDKYQKHLPTVPFEYSKGFQKKRIDHRHHAMDALVIACA
                               TRDHLNLMNNQSAKSELKRYDLQNKLRKKEPYFNQKENKQKEAFKDFIKPWGTFTEDS
                               KNALEKIIISFKQNLRVINKATNSYESYKDEDGNLRIGKDGKPEKGLIKQKGLNWAIR
                               KPLHKDTVSGQINLSRIKLPNGKILTATRKNLDTSFDLKTIENSITDTGIQKILKNYL
                               LSKESPELAFSPEGLEEMNNEIEKYNNGKFHHPINKARIYELGSKFNVGQTGNRRDKF
                               VEAAKGTNLFFAIYQDENKNRSYETIPLNEVIEHQKWRASLPKEEQEKIPLVPVNKLK
                               GTFIFSLSPNDLVYVPSNDELERSASIDFSKLKKEQINRLYKMVSSSGSQCFFVKSEV
                               ATSVVNKMEYSSLNKMEKSIDNLMVKEICIKLKIDRLGNISKA*"
               misc_feature    1861..1875
                               /label="NLS"
                               /translation="MYPYDVPDYASPKKKRKVEASMTKTILGLDLGTNSIGWALINQD
                               FDNKKGEILGMGSRIIPMTQDIKDEFGKGNSISQTAERTRLRGVRRLIHRTLLRRERL
                               HRVLNIIGFLPEHYANQIDFEKRFGKFKPESEPKISFDGNDVFLFEKSYQEMLVDFKI
                               HQPQLVSNGKKIPHDWTIYYLRKKALSKKIEKEELAWILLNFNQKRGYYQLRGEEEEE
                               TPNKLVEFHSLKIVEVNADEPQKGKPEIWYSLVLENGWVYRRASKTPLFDWKDKIREF
                               IVTTEINNDGTVKTDKEGTEKRSFRAPKPEDWTLQKKKTEFELSKSGNEVGAFIYESI
                               LQKPNQKIRGKLISTIERKFYKEELKTILKTQLFFHKELKDEKLYNACIEELYKNNEA
                               HRSLLSNKGFEHLFINDILFYQRPLRSKKSQISNCPLEKRTYKKEGIEITEGIKVISK
                               SNPIYQEFRLWQWISNLSLYCIEPTETNVTSTFLNSIEDYENLFEFLNNRKEIEQKHL
                               LKYLLENQGFKGKLLTNELEKFRWNFVADKKYPCNETGSLLHTRLSKVKNISPDFLTK
                               EIEHQLWHIIYSVTDKIEYEQALKTFARKNNLDVDSFFEHFKKIPPFESTYGAYSEKA
                               IKKLLPLIRLGKYWNWEAIDSISKDRISKILSGEYDENIKNRVREKAVHLTSENNFQG
                               LQEWLAKYIVYDRHSEGNDLGKWTSVSDLETYLKEFKQHSLRNPIVEQVITETLRVVK
                               DIWIKHGKGTENFFDEIHVELGREMKNNSEDRKRLTNTITENENTNLRIKALLMEMMN
                               DNDVENVRPYSPSQQEILKIYEDGALNSNIELDDEIVKISKKAEPTKSELQRYKLWLE
                               QKYRSPYTGQVIPLNKLFTSEYEIEHVVPQSRFFDDSFSNKVICESAVNKRKDNQLGL
                               QFIKNHSGEKVELGFGKVVQVFTEEQYLDFVKEHYSKNRSKHNKLLLEEIPEKMIERQ
                               LNDTRYISKFVSSILSNIVRSEKDDDGLNSKNILPGNGKITTELKRDWGLNDVWNDLI
                               LPRFERMNTITNSDLFTTYNDKYQKHLPTVPFEYSKGFQKKRIDHRHHAMDALVIACA
                               TRDHLNLMNNQSAKSELKRYDLQNKLRKKEPYFNQKENKQKEAFKDFIKPWGTFTEDS
                               KNALEKIIISFKQNLRVINKATNSYESYKDEDGNLRIGKDGKPEKGLIKQKGLNWAIR
                               KPLHKDTVSGQINLSRIKLPNGKILTATRKNLDTSFDLKTIENSITDTGIQKILKNYL
                               LSKESPELAFSPEGLEEMNNEIEKYNNGKFHHPINKARIYELGSKFNVGQTGNRRDKF
                               VEAAKGTNLFFAIYQDENKNRSYETIPLNEVIEHQKWRASLPKEEQEKIPLVPVNKLK
                               GTFIFSLSPNDLVYVPSNDELERSASIDFSKLKKEQINRLYKMVSSSGSQCFFVKSEV
                               ATSVVNKMEYSSLNKMEKSIDNLMVKEICIKLKIDRLGNISKA*"
               CDS             complement(4525..5097)
                               /label="ORF frame 3"
                               /translation="MIPCGTCDDQCVHSMVSVINALFLKALGVLERHGGQVFLILVVV
                               SCKQIAVCDGIHPLESWQNQVVPHIIQPPVPFQFGSDLAISRQNVFAIQAVIVLLRSD
                               DVGQNGAHELTDVASVIQLPFDHLLGNLFQQQFVMLGAIFAIVLLHKIQVLLFCKDLD
                               HLPEAQLHFFSAMVLDELQPQLVILPLVDG*"
               misc_feature    complement(6294..6311)
                               /label="Sp6_primer"
                               /translation="MIPCGTCDDQCVHSMVSVINALFLKALGVLERHGGQVFLILVVV
                               SCKQIAVCDGIHPLESWQNQVVPHIIQPPVPFQFGSDLAISRQNVFAIQAVIVLLRSD
                               DVGQNGAHELTDVASVIQLPFDHLLGNLFQQQFVMLGAIFAIVLLHKIQVLLFCKDLD
                               HLPEAQLHFFSAMVLDELQPQLVILPLVDG*"
               misc_feature    6316..6340
                               /label="attB2"
                               /translation="MIPCGTCDDQCVHSMVSVINALFLKALGVLERHGGQVFLILVVV
                               SCKQIAVCDGIHPLESWQNQVVPHIIQPPVPFQFGSDLAISRQNVFAIQAVIVLLRSD
                               DVGQNGAHELTDVASVIQLPFDHLLGNLFQQQFVMLGAIFAIVLLHKIQVLLFCKDLD
                               HLPEAQLHFFSAMVLDELQPQLVILPLVDG*"
               terminator      6352..6818
                               /label="hGH_PA_terminator"
                               /translation="MIPCGTCDDQCVHSMVSVINALFLKALGVLERHGGQVFLILVVV
                               SCKQIAVCDGIHPLESWQNQVVPHIIQPPVPFQFGSDLAISRQNVFAIQAVIVLLRSD
                               DVGQNGAHELTDVASVIQLPFDHLLGNLFQQQFVMLGAIFAIVLLHKIQVLLFCKDLD
                               HLPEAQLHFFSAMVLDELQPQLVILPLVDG*"
               terminator      6824..6859
                               /label="T7_terminator"
                               /translation="MIPCGTCDDQCVHSMVSVINALFLKALGVLERHGGQVFLILVVV
                               SCKQIAVCDGIHPLESWQNQVVPHIIQPPVPFQFGSDLAISRQNVFAIQAVIVLLRSD
                               DVGQNGAHELTDVASVIQLPFDHLLGNLFQQQFVMLGAIFAIVLLHKIQVLLFCKDLD
                               HLPEAQLHFFSAMVLDELQPQLVILPLVDG*"
               rep_origin      6976..7282
                               /label="f1_origin"
                               /translation="MIPCGTCDDQCVHSMVSVINALFLKALGVLERHGGQVFLILVVV
                               SCKQIAVCDGIHPLESWQNQVVPHIIQPPVPFQFGSDLAISRQNVFAIQAVIVLLRSD
                               DVGQNGAHELTDVASVIQLPFDHLLGNLFQQQFVMLGAIFAIVLLHKIQVLLFCKDLD
                               HLPEAQLHFFSAMVLDELQPQLVILPLVDG*"
               misc_feature    complement(7396..7416)
                               /label="pBABE_3_primer"
                               /translation="MIPCGTCDDQCVHSMVSVINALFLKALGVLERHGGQVFLILVVV
                               SCKQIAVCDGIHPLESWQNQVVPHIIQPPVPFQFGSDLAISRQNVFAIQAVIVLLRSD
                               DVGQNGAHELTDVASVIQLPFDHLLGNLFQQQFVMLGAIFAIVLLHKIQVLLFCKDLD
                               HLPEAQLHFFSAMVLDELQPQLVILPLVDG*"
               misc_feature    complement(7402..7617)
                               /label="SV40_enhancer"
                               /translation="MIPCGTCDDQCVHSMVSVINALFLKALGVLERHGGQVFLILVVV
                               SCKQIAVCDGIHPLESWQNQVVPHIIQPPVPFQFGSDLAISRQNVFAIQAVIVLLRSD
                               DVGQNGAHELTDVASVIQLPFDHLLGNLFQQQFVMLGAIFAIVLLHKIQVLLFCKDLD
                               HLPEAQLHFFSAMVLDELQPQLVILPLVDG*"
               promoter        7414..7682
                               /label="SV40_promoter"
                               /translation="MIPCGTCDDQCVHSMVSVINALFLKALGVLERHGGQVFLILVVV
                               SCKQIAVCDGIHPLESWQNQVVPHIIQPPVPFQFGSDLAISRQNVFAIQAVIVLLRSD
                               DVGQNGAHELTDVASVIQLPFDHLLGNLFQQQFVMLGAIFAIVLLHKIQVLLFCKDLD
                               HLPEAQLHFFSAMVLDELQPQLVILPLVDG*"
               rep_origin      7581..7658
                               /label="SV40_origin"
                               /translation="MIPCGTCDDQCVHSMVSVINALFLKALGVLERHGGQVFLILVVV
                               SCKQIAVCDGIHPLESWQNQVVPHIIQPPVPFQFGSDLAISRQNVFAIQAVIVLLRSD
                               DVGQNGAHELTDVASVIQLPFDHLLGNLFQQQFVMLGAIFAIVLLHKIQVLLFCKDLD
                               HLPEAQLHFFSAMVLDELQPQLVILPLVDG*"
               misc_feature    7643..7662
                               /label="SV40pro_F_primer"
                               /translation="MIPCGTCDDQCVHSMVSVINALFLKALGVLERHGGQVFLILVVV
                               SCKQIAVCDGIHPLESWQNQVVPHIIQPPVPFQFGSDLAISRQNVFAIQAVIVLLRSD
                               DVGQNGAHELTDVASVIQLPFDHLLGNLFQQQFVMLGAIFAIVLLHKIQVLLFCKDLD
                               HLPEAQLHFFSAMVLDELQPQLVILPLVDG*"
               CDS             7797..8591
                               /label="ORF frame 3"
                               /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                               PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                               LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                               EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                               QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
               gene            7800..8588
                               /label="NeoR/KanR"
                               /gene="NeoR/KanR"
                               /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                               PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                               LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                               EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                               QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
               CDS             complement(8106..8642)
                               /label="ORF frame 1"
                               /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                               VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                               VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                               PPHCISHDGYFLGRSKVR*"
               terminator      8768..8887
                               /label="SV40_PA_terminator"
                               /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                               VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                               VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                               PPHCISHDGYFLGRSKVR*"
               misc_feature    8856..8875
                               /label="EBV_rev_primer"
                               /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                               VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                               VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                               PPHCISHDGYFLGRSKVR*"
               terminator      9078..9113
                               /label="T7_terminator"
                               /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                               VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                               VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                               PPHCISHDGYFLGRSKVR*"
               misc_feature    complement(9136..9153)
                               /label="Baculovirs_rev_primer"
                               /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                               VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                               VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                               PPHCISHDGYFLGRSKVR*"
               misc_feature    9143..9220
                               /label="3_AcPH"
                               /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                               VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                               VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                               PPHCISHDGYFLGRSKVR*"
               CDS             complement(9250..9867)
                               /label="ORF frame 3"
                               /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                               NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                               QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                               KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
               misc_feature    9847..9880
                               /label="loxP"
                               /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                               NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                               QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                               KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
               gene            complement(10648..11508)
                               /label="Ampicillin"
                               /gene="Ampicillin"
                               /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                               NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                               QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                               KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
               CDS             complement(10648..11508)
                               /label="ORF frame 3"
                               /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                               IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                               YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                               DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                               LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                               EIGASLIKHW*"
          ORIGIN
              1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
             61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
            121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
            181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
            241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
            301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
            361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
            421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
            481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
            541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
            601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
            661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
            721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
            781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
            841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
            901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
            961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccc
           1021 tcgagtagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
           1081 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
           1141 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
           1201 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
           1261 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
           1321 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
           1381 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
           1441 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
           1501 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
           1561 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtgg atcttaatac
           1621 gactcactat aggggaattg tgagcggata acaattccgg gacctttaat tcaacccaac
           1681 acaatatatt atagttaaat aagaattatt atcaaatcat ttgtatatta attaaaatac
           1741 tatactgtaa attacatttt atttacaatc aacaagtttg tacaaaaaag caggctaagg
           1801 agatataccA CCGGTGCCAC CATGTACCCA TACGATGTTC CAGATTACGC TTCGCCGAAG
           1861 AAAAAGCGCA AGGTCGAAGC GTCCATGACA AAGACTATTC TGGGACTGGA TCTGGGGACT
           1921 AACAGCATTG GATGGGCACT GATTAACCAG GACTTCGATA ACAAAAAGGG GGAGATCCTG
           1981 GGCATGGGCA GCCGGATCAT TCCTATGACA CAGGATATTA AGGACGAGTT CGGAAAAGGC
           2041 AACTCTATCA GTCAGACAGC TGAGCGAACT CGACTGCGGG GAGTCCGGAG ACTGATCCAC
           2101 AGAACTCTGC TGAGGCGCGA GCGGCTGCAT AGAGTGCTGA ATATCATTGG CTTCCTGCCA
           2161 GAACACTATG CCAACCAGAT TGACTTTGAG AAGAGGTTCG GCAAGTTCAA GCCTGAATCT
           2221 GAGCCAAAAA TCAGTTTCGA TGGAAATGAC GTGTTCCTGT TTGAGAAGAG CTACCAGGAA
           2281 ATGCTGGTCG ACTTTAAAAT CCACCAGCCA CAGCTGGTGA GCAACGGCAA GAAAATTCCC
           2341 CATGACTGGA CAATCTACTA TCTGAGAAAG AAAGCTCTGT CTAAGAAAAT TGAGAAGGAG
           2401 GAACTGGCAT GGATCCTGCT GAACTTCAAT CAGAAACGGG GATACTATCA GCTGAGAGGC
           2461 GAGGAAGAGG AAGAGACACC AAATAAGCTG GTGGAGTTTC ATAGCCTGAA AATTGTGGAG
           2521 GTCAACGCCG ATGAACCCCA GAAAGGAAAG CCTGAGATCT GGTACTCACT GGTGCTGGAA
           2581 AACGGCTGGG TCTATCGACG GGCTAGCAAG ACTCCCCTGT TCGATTGGAA AGACAAGATT
           2641 AGGGAGTTTA TCGTGACCAC AGAAATCAAC AATGATGGAA CTGTCAAAAC CGACAAGGAG
           2701 GGCACCGAAA AGAGGAGCTT CCGCGCACCA AAACCCGAGG ACTGGACTCT GCAGAAGAAA
           2761 AAGACCGAGT TCGAGCTGTC AAAGAGCGGG AATGAAGTGG GAGCCTTCAT CTACGAAAGT
           2821 ATCCTGCAGA AACCCAACCA GAAGATTAGA GGCAAACTGA TTTCAACCAT CGAGAGGAAA
           2881 TTTTATAAGG AAGAGCTGAA AACCATCCTG AAAACACAGC TGTTCTTTCA CAAAGAACTG
           2941 AAGGATGAGA AACTGTACAA TGCCTGCATC GAAGAGCTGT ATAAGAACAA TGAAGCTCAC
           3001 CGGAGCCTGC TGTCCAACAA GGGGTTCGAG CATCTGTTTA TTAACGACAT CCTGTTCTAC
           3061 CAGCGACCTC TGCGGTCTAA AAAGAGTCAG ATCTCAAACT GCCCACTGGA GAAGCGCACA
           3121 TATAAAAAGG AGGGGATTGA AATCACTGAG GGCATCAAAG TGATCTCCAA ATCTAATCCA
           3181 ATCTACCAGG AGTTCCGGCT GTGGCAGTGG ATTAGCAACC TGTCCCTGTA TTGTATCGAA
           3241 CCCACCGAGA CAAATGTGAC TTCAACCTTT CTGAACAGCA TTGAAGATTA CGAGAATCTG
           3301 TTCGAATTTC TGAACAATCG CAAGGAAATC GAGCAGAAGC ACCTGCTGAA ATATCTGCTG
           3361 GAGAACCAGG GGTTTAAAGG AAAGCTGCTG ACAAACGAAC TGGAGAAGTT CCGCTGGAAT
           3421 TTTGTCGCTG ACAAAAAGTA CCCCTGTAAT GAGACAGGCA GCCTGCTGCA TACTCGGCTG
           3481 AGCAAAGTGA AGAACATTTC CCCTGATTTC CTGACCAAGG AAATCGAGCA CCAGCTGTGG
           3541 CATATCATCT ACAGCGTGAC CGACAAGATT GAATATGAGC AGGCCCTGAA AACCTTTGCT
           3601 CGGAAAAACA ATCTGGATGT GGACTCCTTC TTTGAGCACT TCAAAAAGAT CCCCCCTTTT
           3661 GAGTCTACCT ACGGAGCATA TAGTGAAAAG GCCATCAAGA AGCTGCTGCC ACTGATCAGA
           3721 CTGGGCAAAT ACTGGAACTG GGAGGCCATT GATAGTATCT CAAAGGACAG GATTAGTAAA
           3781 ATCCTGTCAG GGGAATACGA TGAGAACATT AAGAACAGAG TGAGGGAGAA AGCAGTCCAC
           3841 CTGACCTCCG AAAACAATTT CCAGGGACTG CAGGAGTGGC TGGCCAAGTA CATCGTCTAT
           3901 GATCGCCATT CTGAGGGCAA TGACCTGGGG AAGTGGACTA GCGTGTCCGA CCTGGAGACA
           3961 TACCTGAAGG AGTTCAAGCA GCATAGCCTG CGGAACCCTA TTGTGGAGCA GGTCATCACA
           4021 GAAACTCTGA GAGTGGTCAA GGATATTTGG ATCAAGCACG GGAAAGGAAC CGAAAATTTC
           4081 TTTGACGAAA TCCATGTGGA GCTGGGCCGG GAAATGAAGA ACAATTCCGA GGATCGCAAA
           4141 CGACTGACCA ACACAATTAC TGAAAACGAG AATACAAACC TGAGAATCAA GGCCCTGCTG
           4201 ATGGAAATGA TGAATGATAA CGACGTGGAG AACGTCAGGC CTTACTCTCC AAGTCAGCAG
           4261 GAGATTCTGA AGATCTATGA GGACGGAGCT CTGAATAGCA ACATCGAGCT GGACGATGAA
           4321 ATTGTGAAGA TCTCCAAAAA GGCAGAGCCC ACCAAATCTG AACTGCAGCG CTACAAGCTG
           4381 TGGCTGGAGC AGAAATACCG ATCCCCTTAT ACTGGCCAGG TCATCCCACT GAACAAGCTG
           4441 TTCACCTCTG AATATGAGAT CGAACACGTG GTCCCTCAGA GTCGCTTCTT TGACGATAGC
           4501 TTCAGCAACA AAGTGATCTG CGAGTCAGCC GTCAACAAGC GGAAGGATAA CCAGCTGGGG
           4561 CTGCAGTTCA TCAAGAACCA TAGCGGAGAA AAAGTGGAGC TGGGCTTCGG GAAGGTGGTC
           4621 CAGGTCTTTA CAGAAGAGCA GTACCTGGAT TTTGTGAAGG AGCACTATAG CAAAAATCGC
           4681 TCCAAGCATA ACAAACTGCT GCTGGAAGAG ATTCCCGAGA AGATGATCGA AAGGCAGCTG
           4741 AATGACACTC GCTACATCAG TAAGTTCGTG AGCTCCATTC TGTCCAACAT CGTCAGATCT
           4801 GAGAAGGACG ATGACGGCCT GAATAGCAAA AACATTCTGC CTGGAAATGG CAAGATCACT
           4861 ACCGAACTGA AAAGGGACTG GGGGCTGAAT GATGTGTGGA ACGACCTGAT TCTGCCAAGA
           4921 TTCGAGAGGA TGAATACCAT CACAAACAGC GATCTGTTTA CAACTTACAA CGACAAGTAT
           4981 CAGAAACACC TGCCCACCGT GCCTTTCGAG TACTCCAAGG GCTTTCAGAA AAAGCGCATT
           5041 GATCACCGAC ACCATGCTAT GGACGCACTG GTCATCGCAT GTGCCACACG GGATCATCTG
           5101 AATCTGATGA ACAATCAGTC TGCCAAGAGT GAACTGAAAC GATACGACCT GCAGAACAAG
           5161 CTGCGGAAAA AGGAGCCTTA CTTCAACCAG AAGGAGAACA AACAGAAGGA AGCCTTCAAG
           5221 GATTTTATCA AACCATGGGG CACTTTCACC GAGGACAGCA AGAATGCTCT GGAAAAAATC
           5281 ATTATCTCCT TTAAGCAGAA CCTGAGAGTG ATCAACAAGG CAACAAACTC ATACGAGAGC
           5341 TATAAGGATG AGGACGGGAA TCTGAGGATC GGCAAGGATG GGAAACCAGA GAAGGGCCTG
           5401 ATCAAGCAGA AGGGGCTGAA CTGGGCAATC AGAAAGCCCC TGCACAAAGA CACCGTGTCA
           5461 GGCCAGATTA ACCTGAGCAG GATCAAGCTG CCCAATGGGA AAATCCTGAC AGCCACTCGC
           5521 AAGAATCTGG ATACCAGTTT CGACCTGAAA ACAATTGAGA ACTCAATCAC CGACACAGGC
           5581 ATTCAGAAAA TCCTGAAGAA TTACCTGCTG TCCAAGGAAT CTCCAGAGCT GGCCTTCTCT
           5641 CCCGAGGGCC TGGAAGAGAT GAACAATGAG ATCGAAAAGT ACAACAATGG GAAATTTCAC
           5701 CATCCTATTA ACAAGGCTAG GATCTATGAA CTGGGGAGCA AATTCAATGT CGGACAGACA
           5761 GGCAACAGAA GGGATAAGTT TGTGGAGGCC GCTAAAGGAA CTAATCTGTT CTTTGCTATC
           5821 TACCAGGACG AGAATAAGAA CCGCTCTTAT GAAACTATTC CCCTGAACGA AGTGATCGAG
           5881 CACCAGAAGT GGCGAGCAAG TCTGCCTAAG GAAGAGCAGG AGAAAATTCC ACTGGTGCCC
           5941 GTCAACAAGC TGAAGGGGAC CTTCATCTTT TCCCTGTCTC CCAACGATCT GGTGTACGTC
           6001 CCTTCCAATG ACGAGCTGGA ACGAAGTGCT TCAATTGACT TCTCTAAGCT GAAAAAGGAA
           6061 CAGATCAACC GGCTGTATAA AATGGTGTCT AGTTCAGGAT CCCAGTGCTT CTTTGTGAAG
           6121 AGTGAGGTCG CAACCTCAGT GGTCAACAAA ATGGAATACA GCAGCCTGAA CAAGATGGAG
           6181 AAGTCTATCG ATAACCTGAT GGTGAAGGAG ATTTGTATCA AACTGAAGAT TGACAGGCTG
           6241 GGCAACATCA GCAAGGCCTA AGAATTCggt accgagctca agcttgcggc cgcctatagt
           6301 gtcacctaaa tcgtaaccca gctttcttgt acaaagtggt cgggtggcat ccctgtgacc
           6361 cctccccagt gcctctcctg gccctggaag ttgccactcc agtgcccacc agccttgtcc
           6421 taataaaatt aagttgcatc attttgtctg actaggtgtc cttctataat attatggggt
           6481 ggaggggggt ggtatggagc aaggggcaag ttgggatgac aacctgtagg gcctgcgggg
           6541 tctattggga accaagctgg agtgcagtgg cacaatcttg gctcactgca atctccgcct
           6601 cctgggttca agcgattctc ctgcctcagc ctcccgagtt gttgggattc caggcatgca
           6661 tgaccaggct cagctaattt ttgttttttt ggtagagacg gggtttcacc atattggcca
           6721 ggctggtctc caactcctaa tctcaggtga tctacccacc ttggcctccc aaattgctgg
           6781 gattacaggc gtgaaccact gctcccttcc ctgtccttct agcataaccc cttggggcct
           6841 ctaaacgggt cttgaggggt tttttgtcta gaagacaata gcaggcatgc tggggatgcg
           6901 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
           6961 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
           7021 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
           7081 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
           7141 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
           7201 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
           7261 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
           7321 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
           7381 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
           7441 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
           7501 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
           7561 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
           7621 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
           7681 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
           7741 gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga
           7801 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
           7861 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
           7921 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
           7981 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
           8041 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
           8101 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
           8161 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
           8221 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
           8281 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg
           8341 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
           8401 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
           8461 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
           8521 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
           8581 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
           8641 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
           8701 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
           8761 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
           8821 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
           8881 atcttatcat gtctgttcat gaagcccctt gagcatctga cttctggcta ataaaggaaa
           8941 tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat
           9001 gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat
           9061 gcccatatgt aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg
           9121 ctgaaagcat gcggaggaaa ttctccttga agtttccctg gtgttcaaag taaaggagtt
           9181 tgcaccagac gcacctctgt tcactggtcc ggcgtattaa aacacgatac attgttatta
           9241 gtacatttat taagcgctag attctgtgcg ttgttgattt acagacaatt gttgtacgta
           9301 ttttaataat tcattaaatt tataatcttt agggtggtat gttagagcga aaatcaaatg
           9361 attttcagcg tctttatatc tgaatttaaa tattaaatcc tcaatagatt tgtaaaatag
           9421 gtttcgatta gtttcaaaca agggttgttt ttccgaaccg atggctggac tatctaatgg
           9481 attttcgctc aacgccacaa aacttgccaa atcttgtagc agcaatctag ctttgtcgat
           9541 attcgtttgt gttttgtttt gtaataaagg ttcgacgtcg ttcaaaatat tatgcgcttt
           9601 tgtatttctt tcatcactgt cgttagtgta caattgactc gacgtaaaca cgttaaatag
           9661 agcttggaca tatttaacat cgggcgtgtt agctttatta ggccgattat cgtcgtcgtc
           9721 ccaaccctcg tcgttagaag ttgcttccga agacgatttt gccatagcca cacgacgcct
           9781 attaattgtg tcggctaaca cgtccgcgat caaatttgta gttgagcttt ttggaattgc
           9841 gatcgcataa cttcgtatag catacattat acgaagttat aagctcggaa cgctgcgctc
           9901 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
           9961 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
          10021 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgccccctga cgagcatcac
          10081 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
          10141 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
          10201 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat
          10261 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
          10321 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
          10381 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
          10441 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
          10501 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
          10561 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
          10621 aaaaaaggat ctcaagaaga tcctttgtta ccaatgctta atcagtgagg cacctatctc
          10681 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
          10741 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
          10801 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
          10861 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
          10921 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
          10981 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
          11041 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
          11101 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
          11161 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
          11221 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
          11281 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
          11341 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
          11401 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
          11461 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
          11521 tcaatattat tgaagcattt atcagggtta ttgtctcatg tccgcgcgtt
          //
          
          

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