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          PX388

          價格:1000元

          聯系方式:I47-825O-882O

          買家導航

          載體基本信息

          出品公司: Addgene
          載體名稱: PX388 或 PX388 Bacteroides coprophilus Cas9
          質粒類型: CRISPR-Cas9載體;基因敲除載體;昆蟲細胞載體
          高拷貝/低拷貝: 高拷貝
          插入位點: 限制性內切酶,多克隆位點
          啟動子: CMV
          載體大小: 11723 bp
          5' 測序引物及序列: CMV Forward:CGCAAATGGGCGGTAGGCGTG
          3' 測序引物及序列: --
          載體標簽: NLS (C ter);HA、NLS (N ter)
          載體抗性: 氨芐青霉素
          篩選標記: Neomycin
          克隆菌株: Stbl3
          宿主細胞(系): 昆蟲細胞
          備注: --
          產品目錄號: #68325
          穩定性: 穩表達
          組成型/誘導型: 組成型
          病毒/非病毒: 桿狀病毒

          載體質粒圖譜和多克隆位點信息

          PX388載體圖譜



          載體簡介

          載體序列

          LOCUS       PX388 Bacteroides coprophilus Cas9	11723 bp 	DNA	SYN
          DEFINITION  PX388 Bacteroides coprophilus Cas9
          ACCESSION   
          KEYWORDS    
          SOURCE      
            ORGANISM  other sequences; artificial sequences; vectors.
          FEATURES             Location/Qualifiers
               source          1..11723
                               /organism="PX388 Bacteroides coprophilus Cas9"
                               /mol_type="other DNA"
               misc_feature    47..1001
                               /label="Ac_5_flank"
               CDS             complement(343..948)
                               /label="ORF frame 3"
                               /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                               VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                               PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                               LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
               promoter        1023..1587
                               /label="CMV_immearly_promoter"
                               /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                               VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                               PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                               LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
               misc_feature    1090..1377
                               /label="CAG_enhancer"
                               /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                               VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                               PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                               LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
               misc_feature    1544..1564
                               /label="CMV_fwd_primer"
                               /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                               VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                               PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                               LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
               promoter        1615..1633
                               /label="T7_promoter"
                               /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                               VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                               PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                               LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
               misc_feature    1633..1656
                               /label="lacO"
                               /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                               VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                               PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                               LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
               misc_feature    1772..1796
                               /label="attB1"
                               /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                               VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                               PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                               LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
               CDS             1822..6414
                               /label="ORF frame 1"
                               /translation="MYPYDVPDYASPKKKRKVEASMKKTLGLDLGTNSIGWAVINSNI
                               DSEGKEKLVGISSCGSRIIPMDATTLGDFGKGNTKSPVAERTRLRGIRRLLERSLLRR
                               ERLHRVLSVMGFLPEHYASQLDRYGKFLPETEPKLAWYKDDSGRYQFLFQKSFHEMLE
                               DFRQHQPELVAGEKKIPYDWTIYYLRKKALSQEITKEELAWILLNFNQKRGYYQLRGE
                               EEQEENNKSVEYHALKVVSVEDSGERKGKDIWYNVTLENGWVYRRASNIPLDWTGKVK
                               EFVVTTELDDAGNPKKDKEGNVKRSFRAPKEDDWGLLKTRTQAQIDESGKTVGTYIYE
                               SLLCMPNQKIRGKLVRTIERKYYKDELRQILVKQSEFHAALQDHNLLLSCIEELYPNN
                               EAHRRLLSASSFIYFLIEDILFYQRPLKSQKGLIDNCPYESHIYKDKKDGSLHHVPLK
                               CVSKSHPLFQEFRLWQFLSNLRIYQRERMMDGSLKLDVDVTREFLPSEEDYVKLFDWL
                               NERKEISQKFLLAYKPFGLKKNEQANYRWNYVEDKSYPCNETRAEIKSRLSKAGVPEE
                               FLTEEKEEALWHILYSISDKKELTKALGTFAAKNCLNESFVEVFAKIPPFESNYAAYS
                               LKAIRKLLALMRMGKYWNEQAIDRQTRDRIEKILTGEYDETIRSRVREKAMLLTDISS
                               FRGLPLWLACYIVYDRHSESKELVKWETPADIDHFLSKFKQNSLRNPIVEQVITESLR
                               TVRDIWKQEGKIDEIHVELGREMKNPAKERARITAQVQENENTNLRIKALLAEFMNPE
                               FEIENVHPYSPGQQEILRIYEDGVLSGIAEKDLPDDITAILKKFRENDVKKRPTTSEV
                               LRYKLWLEQRYRSPYTGRVIPLGKLFTPAYEIEHVIPQSRYFDDSISNKVICESAVNK
                               LKDNCLGYEFIKKHSGEMVELGNGETVPVFSVEEYERFVKESYFGNSKKMKKLLLEDI
                               PDSFIERQLNDSRYISRVVTSLLSNLVCEEGEQDGLSKNVIVCTGGITDRLKKDWGVQ
                               EVWNRIILPRFLRLNEITGRTDFTSTSVNGHLLPALPLYLQKGFNKKRIDHRHHAMDA
                               IVIACANRNIVNYLNNSSARKNSEISRYDLQRLLCEKVKTDANGNYKWILRKPWETFT
                               QDVYAALTNIVVSFKQNLRVINRTTNYYQHYNEAGEKRMIPQTKGDRWAIRKPMHKDT
                               VYGEVNLRKEKTLPLRDVVKNPSIVVDKSLKNKLYELLKSQYDLKAIAKYFETHQDVW
                               ADVNLKKIKVYYFTKETNERFFATRKSLDPSFDQKKIEEEVTDTGVQKILLHHLQQNN
                               NDPDMAFSPDGIDRMNQNMTILNDGKWHQPIYKVRTYEKADKFAVGESGNKAKKFVEA
                               AKGTNLFFAVYESVQEDEAIGKQVCKRTFATIPLNEVIKRKKQGLPAAPEDLNGNLPK
                               FVLSPNDLVYLPTEEERNSSRIIQPLDRERIYKMVSSSGSQCFFIKVFVANSIWDKNE
                               YSSLNKMERAITNEMIKEICVPIKIDRLGNVSLIQI*"
               misc_feature    1825..1851
                               /label="HA"
                               /translation="MYPYDVPDYASPKKKRKVEASMKKTLGLDLGTNSIGWAVINSNI
                               DSEGKEKLVGISSCGSRIIPMDATTLGDFGKGNTKSPVAERTRLRGIRRLLERSLLRR
                               ERLHRVLSVMGFLPEHYASQLDRYGKFLPETEPKLAWYKDDSGRYQFLFQKSFHEMLE
                               DFRQHQPELVAGEKKIPYDWTIYYLRKKALSQEITKEELAWILLNFNQKRGYYQLRGE
                               EEQEENNKSVEYHALKVVSVEDSGERKGKDIWYNVTLENGWVYRRASNIPLDWTGKVK
                               EFVVTTELDDAGNPKKDKEGNVKRSFRAPKEDDWGLLKTRTQAQIDESGKTVGTYIYE
                               SLLCMPNQKIRGKLVRTIERKYYKDELRQILVKQSEFHAALQDHNLLLSCIEELYPNN
                               EAHRRLLSASSFIYFLIEDILFYQRPLKSQKGLIDNCPYESHIYKDKKDGSLHHVPLK
                               CVSKSHPLFQEFRLWQFLSNLRIYQRERMMDGSLKLDVDVTREFLPSEEDYVKLFDWL
                               NERKEISQKFLLAYKPFGLKKNEQANYRWNYVEDKSYPCNETRAEIKSRLSKAGVPEE
                               FLTEEKEEALWHILYSISDKKELTKALGTFAAKNCLNESFVEVFAKIPPFESNYAAYS
                               LKAIRKLLALMRMGKYWNEQAIDRQTRDRIEKILTGEYDETIRSRVREKAMLLTDISS
                               FRGLPLWLACYIVYDRHSESKELVKWETPADIDHFLSKFKQNSLRNPIVEQVITESLR
                               TVRDIWKQEGKIDEIHVELGREMKNPAKERARITAQVQENENTNLRIKALLAEFMNPE
                               FEIENVHPYSPGQQEILRIYEDGVLSGIAEKDLPDDITAILKKFRENDVKKRPTTSEV
                               LRYKLWLEQRYRSPYTGRVIPLGKLFTPAYEIEHVIPQSRYFDDSISNKVICESAVNK
                               LKDNCLGYEFIKKHSGEMVELGNGETVPVFSVEEYERFVKESYFGNSKKMKKLLLEDI
                               PDSFIERQLNDSRYISRVVTSLLSNLVCEEGEQDGLSKNVIVCTGGITDRLKKDWGVQ
                               EVWNRIILPRFLRLNEITGRTDFTSTSVNGHLLPALPLYLQKGFNKKRIDHRHHAMDA
                               IVIACANRNIVNYLNNSSARKNSEISRYDLQRLLCEKVKTDANGNYKWILRKPWETFT
                               QDVYAALTNIVVSFKQNLRVINRTTNYYQHYNEAGEKRMIPQTKGDRWAIRKPMHKDT
                               VYGEVNLRKEKTLPLRDVVKNPSIVVDKSLKNKLYELLKSQYDLKAIAKYFETHQDVW
                               ADVNLKKIKVYYFTKETNERFFATRKSLDPSFDQKKIEEEVTDTGVQKILLHHLQQNN
                               NDPDMAFSPDGIDRMNQNMTILNDGKWHQPIYKVRTYEKADKFAVGESGNKAKKFVEA
                               AKGTNLFFAVYESVQEDEAIGKQVCKRTFATIPLNEVIKRKKQGLPAAPEDLNGNLPK
                               FVLSPNDLVYLPTEEERNSSRIIQPLDRERIYKMVSSSGSQCFFIKVFVANSIWDKNE
                               YSSLNKMERAITNEMIKEICVPIKIDRLGNVSLIQI*"
               misc_feature    1855..1875
                               /label="SV40 NLS"
                               /translation="MYPYDVPDYASPKKKRKVEASMKKTLGLDLGTNSIGWAVINSNI
                               DSEGKEKLVGISSCGSRIIPMDATTLGDFGKGNTKSPVAERTRLRGIRRLLERSLLRR
                               ERLHRVLSVMGFLPEHYASQLDRYGKFLPETEPKLAWYKDDSGRYQFLFQKSFHEMLE
                               DFRQHQPELVAGEKKIPYDWTIYYLRKKALSQEITKEELAWILLNFNQKRGYYQLRGE
                               EEQEENNKSVEYHALKVVSVEDSGERKGKDIWYNVTLENGWVYRRASNIPLDWTGKVK
                               EFVVTTELDDAGNPKKDKEGNVKRSFRAPKEDDWGLLKTRTQAQIDESGKTVGTYIYE
                               SLLCMPNQKIRGKLVRTIERKYYKDELRQILVKQSEFHAALQDHNLLLSCIEELYPNN
                               EAHRRLLSASSFIYFLIEDILFYQRPLKSQKGLIDNCPYESHIYKDKKDGSLHHVPLK
                               CVSKSHPLFQEFRLWQFLSNLRIYQRERMMDGSLKLDVDVTREFLPSEEDYVKLFDWL
                               NERKEISQKFLLAYKPFGLKKNEQANYRWNYVEDKSYPCNETRAEIKSRLSKAGVPEE
                               FLTEEKEEALWHILYSISDKKELTKALGTFAAKNCLNESFVEVFAKIPPFESNYAAYS
                               LKAIRKLLALMRMGKYWNEQAIDRQTRDRIEKILTGEYDETIRSRVREKAMLLTDISS
                               FRGLPLWLACYIVYDRHSESKELVKWETPADIDHFLSKFKQNSLRNPIVEQVITESLR
                               TVRDIWKQEGKIDEIHVELGREMKNPAKERARITAQVQENENTNLRIKALLAEFMNPE
                               FEIENVHPYSPGQQEILRIYEDGVLSGIAEKDLPDDITAILKKFRENDVKKRPTTSEV
                               LRYKLWLEQRYRSPYTGRVIPLGKLFTPAYEIEHVIPQSRYFDDSISNKVICESAVNK
                               LKDNCLGYEFIKKHSGEMVELGNGETVPVFSVEEYERFVKESYFGNSKKMKKLLLEDI
                               PDSFIERQLNDSRYISRVVTSLLSNLVCEEGEQDGLSKNVIVCTGGITDRLKKDWGVQ
                               EVWNRIILPRFLRLNEITGRTDFTSTSVNGHLLPALPLYLQKGFNKKRIDHRHHAMDA
                               IVIACANRNIVNYLNNSSARKNSEISRYDLQRLLCEKVKTDANGNYKWILRKPWETFT
                               QDVYAALTNIVVSFKQNLRVINRTTNYYQHYNEAGEKRMIPQTKGDRWAIRKPMHKDT
                               VYGEVNLRKEKTLPLRDVVKNPSIVVDKSLKNKLYELLKSQYDLKAIAKYFETHQDVW
                               ADVNLKKIKVYYFTKETNERFFATRKSLDPSFDQKKIEEEVTDTGVQKILLHHLQQNN
                               NDPDMAFSPDGIDRMNQNMTILNDGKWHQPIYKVRTYEKADKFAVGESGNKAKKFVEA
                               AKGTNLFFAVYESVQEDEAIGKQVCKRTFATIPLNEVIKRKKQGLPAAPEDLNGNLPK
                               FVLSPNDLVYLPTEEERNSSRIIQPLDRERIYKMVSSSGSQCFFIKVFVANSIWDKNE
                               YSSLNKMERAITNEMIKEICVPIKIDRLGNVSLIQI*"
               misc_feature    1861..1875
                               /label="NLS"
                               /translation="MYPYDVPDYASPKKKRKVEASMKKTLGLDLGTNSIGWAVINSNI
                               DSEGKEKLVGISSCGSRIIPMDATTLGDFGKGNTKSPVAERTRLRGIRRLLERSLLRR
                               ERLHRVLSVMGFLPEHYASQLDRYGKFLPETEPKLAWYKDDSGRYQFLFQKSFHEMLE
                               DFRQHQPELVAGEKKIPYDWTIYYLRKKALSQEITKEELAWILLNFNQKRGYYQLRGE
                               EEQEENNKSVEYHALKVVSVEDSGERKGKDIWYNVTLENGWVYRRASNIPLDWTGKVK
                               EFVVTTELDDAGNPKKDKEGNVKRSFRAPKEDDWGLLKTRTQAQIDESGKTVGTYIYE
                               SLLCMPNQKIRGKLVRTIERKYYKDELRQILVKQSEFHAALQDHNLLLSCIEELYPNN
                               EAHRRLLSASSFIYFLIEDILFYQRPLKSQKGLIDNCPYESHIYKDKKDGSLHHVPLK
                               CVSKSHPLFQEFRLWQFLSNLRIYQRERMMDGSLKLDVDVTREFLPSEEDYVKLFDWL
                               NERKEISQKFLLAYKPFGLKKNEQANYRWNYVEDKSYPCNETRAEIKSRLSKAGVPEE
                               FLTEEKEEALWHILYSISDKKELTKALGTFAAKNCLNESFVEVFAKIPPFESNYAAYS
                               LKAIRKLLALMRMGKYWNEQAIDRQTRDRIEKILTGEYDETIRSRVREKAMLLTDISS
                               FRGLPLWLACYIVYDRHSESKELVKWETPADIDHFLSKFKQNSLRNPIVEQVITESLR
                               TVRDIWKQEGKIDEIHVELGREMKNPAKERARITAQVQENENTNLRIKALLAEFMNPE
                               FEIENVHPYSPGQQEILRIYEDGVLSGIAEKDLPDDITAILKKFRENDVKKRPTTSEV
                               LRYKLWLEQRYRSPYTGRVIPLGKLFTPAYEIEHVIPQSRYFDDSISNKVICESAVNK
                               LKDNCLGYEFIKKHSGEMVELGNGETVPVFSVEEYERFVKESYFGNSKKMKKLLLEDI
                               PDSFIERQLNDSRYISRVVTSLLSNLVCEEGEQDGLSKNVIVCTGGITDRLKKDWGVQ
                               EVWNRIILPRFLRLNEITGRTDFTSTSVNGHLLPALPLYLQKGFNKKRIDHRHHAMDA
                               IVIACANRNIVNYLNNSSARKNSEISRYDLQRLLCEKVKTDANGNYKWILRKPWETFT
                               QDVYAALTNIVVSFKQNLRVINRTTNYYQHYNEAGEKRMIPQTKGDRWAIRKPMHKDT
                               VYGEVNLRKEKTLPLRDVVKNPSIVVDKSLKNKLYELLKSQYDLKAIAKYFETHQDVW
                               ADVNLKKIKVYYFTKETNERFFATRKSLDPSFDQKKIEEEVTDTGVQKILLHHLQQNN
                               NDPDMAFSPDGIDRMNQNMTILNDGKWHQPIYKVRTYEKADKFAVGESGNKAKKFVEA
                               AKGTNLFFAVYESVQEDEAIGKQVCKRTFATIPLNEVIKRKKQGLPAAPEDLNGNLPK
                               FVLSPNDLVYLPTEEERNSSRIIQPLDRERIYKMVSSSGSQCFFIKVFVANSIWDKNE
                               YSSLNKMERAITNEMIKEICVPIKIDRLGNVSLIQI*"
               CDS             complement(2149..3189)
                               /label="ORF frame 3"
                               /translation="MGFADTLQRHMVQTSIFLILVDVRLVGAVIDQPFLGLQWALIKQ
                               NVLDQEVDEAAGAQQPSVGLIVRVQLFDTGQQQIMILQSGVELALLHQDLSQLVFIVL
                               AFNGPHQFSPDLLVGHTQQRLVDVSSHGLAGFVDLSLGPSFQQPPIVLLWCPKGSFHI
                               SFLIFLWVPGIVQLGSDHELFHLAGPVQGNVASPSIDPSVLQSHIVPDIFALSFSRVL
                               DAHHFQGVILHALIVLFLLFLPSQLIVAAFLVEIQQNPGQFLLSDLLRQCFLPQIVDC
                               PIVRDFLLSCHQFWLMLSKVLQHLVEGLLKQELIPAAIVFVPCQLGLGFWQKLSIAVQ
                               LASVMLWQEPHD*"
               misc_feature    complement(6447..6464)
                               /label="Sp6_primer"
                               /translation="MGFADTLQRHMVQTSIFLILVDVRLVGAVIDQPFLGLQWALIKQ
                               NVLDQEVDEAAGAQQPSVGLIVRVQLFDTGQQQIMILQSGVELALLHQDLSQLVFIVL
                               AFNGPHQFSPDLLVGHTQQRLVDVSSHGLAGFVDLSLGPSFQQPPIVLLWCPKGSFHI
                               SFLIFLWVPGIVQLGSDHELFHLAGPVQGNVASPSIDPSVLQSHIVPDIFALSFSRVL
                               DAHHFQGVILHALIVLFLLFLPSQLIVAAFLVEIQQNPGQFLLSDLLRQCFLPQIVDC
                               PIVRDFLLSCHQFWLMLSKVLQHLVEGLLKQELIPAAIVFVPCQLGLGFWQKLSIAVQ
                               LASVMLWQEPHD*"
               misc_feature    6469..6493
                               /label="attB2"
                               /translation="MGFADTLQRHMVQTSIFLILVDVRLVGAVIDQPFLGLQWALIKQ
                               NVLDQEVDEAAGAQQPSVGLIVRVQLFDTGQQQIMILQSGVELALLHQDLSQLVFIVL
                               AFNGPHQFSPDLLVGHTQQRLVDVSSHGLAGFVDLSLGPSFQQPPIVLLWCPKGSFHI
                               SFLIFLWVPGIVQLGSDHELFHLAGPVQGNVASPSIDPSVLQSHIVPDIFALSFSRVL
                               DAHHFQGVILHALIVLFLLFLPSQLIVAAFLVEIQQNPGQFLLSDLLRQCFLPQIVDC
                               PIVRDFLLSCHQFWLMLSKVLQHLVEGLLKQELIPAAIVFVPCQLGLGFWQKLSIAVQ
                               LASVMLWQEPHD*"
               terminator      6505..6971
                               /label="hGH_PA_terminator"
                               /translation="MGFADTLQRHMVQTSIFLILVDVRLVGAVIDQPFLGLQWALIKQ
                               NVLDQEVDEAAGAQQPSVGLIVRVQLFDTGQQQIMILQSGVELALLHQDLSQLVFIVL
                               AFNGPHQFSPDLLVGHTQQRLVDVSSHGLAGFVDLSLGPSFQQPPIVLLWCPKGSFHI
                               SFLIFLWVPGIVQLGSDHELFHLAGPVQGNVASPSIDPSVLQSHIVPDIFALSFSRVL
                               DAHHFQGVILHALIVLFLLFLPSQLIVAAFLVEIQQNPGQFLLSDLLRQCFLPQIVDC
                               PIVRDFLLSCHQFWLMLSKVLQHLVEGLLKQELIPAAIVFVPCQLGLGFWQKLSIAVQ
                               LASVMLWQEPHD*"
               terminator      6977..7012
                               /label="T7_terminator"
                               /translation="MGFADTLQRHMVQTSIFLILVDVRLVGAVIDQPFLGLQWALIKQ
                               NVLDQEVDEAAGAQQPSVGLIVRVQLFDTGQQQIMILQSGVELALLHQDLSQLVFIVL
                               AFNGPHQFSPDLLVGHTQQRLVDVSSHGLAGFVDLSLGPSFQQPPIVLLWCPKGSFHI
                               SFLIFLWVPGIVQLGSDHELFHLAGPVQGNVASPSIDPSVLQSHIVPDIFALSFSRVL
                               DAHHFQGVILHALIVLFLLFLPSQLIVAAFLVEIQQNPGQFLLSDLLRQCFLPQIVDC
                               PIVRDFLLSCHQFWLMLSKVLQHLVEGLLKQELIPAAIVFVPCQLGLGFWQKLSIAVQ
                               LASVMLWQEPHD*"
               rep_origin      7129..7435
                               /label="f1_origin"
                               /translation="MGFADTLQRHMVQTSIFLILVDVRLVGAVIDQPFLGLQWALIKQ
                               NVLDQEVDEAAGAQQPSVGLIVRVQLFDTGQQQIMILQSGVELALLHQDLSQLVFIVL
                               AFNGPHQFSPDLLVGHTQQRLVDVSSHGLAGFVDLSLGPSFQQPPIVLLWCPKGSFHI
                               SFLIFLWVPGIVQLGSDHELFHLAGPVQGNVASPSIDPSVLQSHIVPDIFALSFSRVL
                               DAHHFQGVILHALIVLFLLFLPSQLIVAAFLVEIQQNPGQFLLSDLLRQCFLPQIVDC
                               PIVRDFLLSCHQFWLMLSKVLQHLVEGLLKQELIPAAIVFVPCQLGLGFWQKLSIAVQ
                               LASVMLWQEPHD*"
               misc_feature    complement(7549..7569)
                               /label="pBABE_3_primer"
                               /translation="MGFADTLQRHMVQTSIFLILVDVRLVGAVIDQPFLGLQWALIKQ
                               NVLDQEVDEAAGAQQPSVGLIVRVQLFDTGQQQIMILQSGVELALLHQDLSQLVFIVL
                               AFNGPHQFSPDLLVGHTQQRLVDVSSHGLAGFVDLSLGPSFQQPPIVLLWCPKGSFHI
                               SFLIFLWVPGIVQLGSDHELFHLAGPVQGNVASPSIDPSVLQSHIVPDIFALSFSRVL
                               DAHHFQGVILHALIVLFLLFLPSQLIVAAFLVEIQQNPGQFLLSDLLRQCFLPQIVDC
                               PIVRDFLLSCHQFWLMLSKVLQHLVEGLLKQELIPAAIVFVPCQLGLGFWQKLSIAVQ
                               LASVMLWQEPHD*"
               misc_feature    complement(7555..7770)
                               /label="SV40_enhancer"
                               /translation="MGFADTLQRHMVQTSIFLILVDVRLVGAVIDQPFLGLQWALIKQ
                               NVLDQEVDEAAGAQQPSVGLIVRVQLFDTGQQQIMILQSGVELALLHQDLSQLVFIVL
                               AFNGPHQFSPDLLVGHTQQRLVDVSSHGLAGFVDLSLGPSFQQPPIVLLWCPKGSFHI
                               SFLIFLWVPGIVQLGSDHELFHLAGPVQGNVASPSIDPSVLQSHIVPDIFALSFSRVL
                               DAHHFQGVILHALIVLFLLFLPSQLIVAAFLVEIQQNPGQFLLSDLLRQCFLPQIVDC
                               PIVRDFLLSCHQFWLMLSKVLQHLVEGLLKQELIPAAIVFVPCQLGLGFWQKLSIAVQ
                               LASVMLWQEPHD*"
               promoter        7567..7835
                               /label="SV40_promoter"
                               /translation="MGFADTLQRHMVQTSIFLILVDVRLVGAVIDQPFLGLQWALIKQ
                               NVLDQEVDEAAGAQQPSVGLIVRVQLFDTGQQQIMILQSGVELALLHQDLSQLVFIVL
                               AFNGPHQFSPDLLVGHTQQRLVDVSSHGLAGFVDLSLGPSFQQPPIVLLWCPKGSFHI
                               SFLIFLWVPGIVQLGSDHELFHLAGPVQGNVASPSIDPSVLQSHIVPDIFALSFSRVL
                               DAHHFQGVILHALIVLFLLFLPSQLIVAAFLVEIQQNPGQFLLSDLLRQCFLPQIVDC
                               PIVRDFLLSCHQFWLMLSKVLQHLVEGLLKQELIPAAIVFVPCQLGLGFWQKLSIAVQ
                               LASVMLWQEPHD*"
               rep_origin      7734..7811
                               /label="SV40_origin"
                               /translation="MGFADTLQRHMVQTSIFLILVDVRLVGAVIDQPFLGLQWALIKQ
                               NVLDQEVDEAAGAQQPSVGLIVRVQLFDTGQQQIMILQSGVELALLHQDLSQLVFIVL
                               AFNGPHQFSPDLLVGHTQQRLVDVSSHGLAGFVDLSLGPSFQQPPIVLLWCPKGSFHI
                               SFLIFLWVPGIVQLGSDHELFHLAGPVQGNVASPSIDPSVLQSHIVPDIFALSFSRVL
                               DAHHFQGVILHALIVLFLLFLPSQLIVAAFLVEIQQNPGQFLLSDLLRQCFLPQIVDC
                               PIVRDFLLSCHQFWLMLSKVLQHLVEGLLKQELIPAAIVFVPCQLGLGFWQKLSIAVQ
                               LASVMLWQEPHD*"
               misc_feature    7796..7815
                               /label="SV40pro_F_primer"
                               /translation="MGFADTLQRHMVQTSIFLILVDVRLVGAVIDQPFLGLQWALIKQ
                               NVLDQEVDEAAGAQQPSVGLIVRVQLFDTGQQQIMILQSGVELALLHQDLSQLVFIVL
                               AFNGPHQFSPDLLVGHTQQRLVDVSSHGLAGFVDLSLGPSFQQPPIVLLWCPKGSFHI
                               SFLIFLWVPGIVQLGSDHELFHLAGPVQGNVASPSIDPSVLQSHIVPDIFALSFSRVL
                               DAHHFQGVILHALIVLFLLFLPSQLIVAAFLVEIQQNPGQFLLSDLLRQCFLPQIVDC
                               PIVRDFLLSCHQFWLMLSKVLQHLVEGLLKQELIPAAIVFVPCQLGLGFWQKLSIAVQ
                               LASVMLWQEPHD*"
               CDS             7950..8744
                               /label="ORF frame 3"
                               /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                               PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                               LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                               EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                               QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
               gene            7953..8741
                               /label="NeoR/KanR"
                               /gene="NeoR/KanR"
                               /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                               PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                               LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                               EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                               QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
               CDS             complement(8259..8795)
                               /label="ORF frame 1"
                               /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                               VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                               VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                               PPHCISHDGYFLGRSKVR*"
               terminator      8921..9040
                               /label="SV40_PA_terminator"
                               /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                               VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                               VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                               PPHCISHDGYFLGRSKVR*"
               misc_feature    9009..9028
                               /label="EBV_rev_primer"
                               /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                               VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                               VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                               PPHCISHDGYFLGRSKVR*"
               terminator      9231..9266
                               /label="T7_terminator"
                               /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                               VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                               VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                               PPHCISHDGYFLGRSKVR*"
               misc_feature    complement(9289..9306)
                               /label="Baculovirs_rev_primer"
                               /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                               VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                               VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                               PPHCISHDGYFLGRSKVR*"
               misc_feature    9296..9373
                               /label="3_AcPH"
                               /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                               VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                               VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                               PPHCISHDGYFLGRSKVR*"
               CDS             complement(9403..10020)
                               /label="ORF frame 3"
                               /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                               NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                               QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                               KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
               misc_feature    10000..10033
                               /label="loxP"
                               /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                               NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                               QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                               KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
               gene            complement(10801..11661)
                               /label="Ampicillin"
                               /gene="Ampicillin"
                               /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                               NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                               QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                               KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
               CDS             complement(10801..11661)
                               /label="ORF frame 3"
                               /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                               IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                               YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                               DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                               LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                               EIGASLIKHW*"
          ORIGIN
              1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
             61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
            121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
            181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
            241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
            301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
            361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
            421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
            481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
            541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
            601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
            661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
            721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
            781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
            841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
            901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
            961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccc
           1021 tcgagtagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
           1081 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
           1141 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
           1201 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
           1261 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
           1321 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
           1381 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
           1441 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
           1501 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
           1561 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtgg atcttaatac
           1621 gactcactat aggggaattg tgagcggata acaattccgg gacctttaat tcaacccaac
           1681 acaatatatt atagttaaat aagaattatt atcaaatcat ttgtatatta attaaaatac
           1741 tatactgtaa attacatttt atttacaatc aacaagtttg tacaaaaaag caggctaagg
           1801 agatataccA CCGGTGCCAC CATGTACCCA TACGATGTTC CAGATTACGC TTCGCCGAAG
           1861 AAAAAGCGCA AGGTCGAAGC GTCCATGAAG AAAACACTGG GACTGGACCT GGGGACTAAC
           1921 AGCATTGGGT GGGCCGTCAT CAACTCAAAC ATCGACTCAG AAGGAAAAGA AAAGCTGGTG
           1981 GGGATCAGCT CCTGCGGAAG CCGGATCATT CCTATGGACG CTACCACACT GGGAGATTTC
           2041 GGAAAGGGCA ACACTAAAAG TCCAGTGGCA GAGCGAACCC GACTGCGGGG CATTCGGAGA
           2101 CTGCTGGAAA GGTCACTGCT GAGGCGCGAG CGACTGCACC GAGTGCTGTC AGTCATGGGG
           2161 TTCCTGCCAG AGCATTACGC TAGCCAGCTG GACCGCTATG GAAAGTTTCT GCCAGAAACC
           2221 GAGCCCAAGC TGGCATGGTA CAAAGACGAT AGCGGCAGGT ATCAGTTCCT GTTTCAGAAG
           2281 TCCTTCCACG AGATGCTGGA GGACTTTCGA CAGCATCAGC CAGAACTGGT GGCAGGAGAG
           2341 AAGAAAATCC CTTACGATTG GACAATCTAC TATCTGCGGA AGAAAGCACT GTCTCAGGAG
           2401 ATCACTAAGG AGGAACTGGC CTGGATTCTG CTGAATTTCA ACCAGAAACG CGGCTACTAT
           2461 CAGCTGCGAG GGGAGGAAGA GCAGGAAGAG AACAATAAGA GCGTGGAGTA TCACGCCCTG
           2521 AAAGTGGTGA GCGTCGAGGA CTCTGGAGAA AGAAAGGGCA AAGATATCTG GTACAATGTG
           2581 ACTCTGGAGA ACGGATGGGT CTATCGACGG GCTAGCAACA TTCCCCTGGA CTGGACCGGC
           2641 AAGGTGAAAG AGTTCGTGGT CACTACCGAG CTGGACGATG CCGGGAACCC AAAGAAAGAT
           2701 AAGGAAGGAA ATGTGAAAAG ATCCTTTAGG GCACCAAAGG AGGACGATTG GGGGCTGCTG
           2761 AAAACTAGGA CCCAGGCTCA GATCGACGAA TCCGGCAAGA CCGTGGGAAC TTACATCTAC
           2821 GAGTCTCTGC TGTGTATGCC CAACCAGAAG ATCCGGGGAA AACTGGTGAG GACCATTGAA
           2881 CGCAAGTACT ATAAAGACGA GCTGAGACAG ATCCTGGTGA AGCAGAGCGA GTTCCACGCC
           2941 GCTCTGCAGG ATCATAATCT GCTGCTGTCC TGTATCGAAG AGCTGTACCC TAACAATGAG
           3001 GCCCACAGAA GGCTGCTGAG CGCCAGCAGC TTCATCTACT TCCTGATCGA GGACATTCTG
           3061 TTTTATCAGC GCCCACTGAA GTCCCAGAAA GGGCTGATCG ATAACTGCCC CTACGAGTCT
           3121 CACATCTACA AGGATAAGAA AGATGGAAGT CTGCACCATG TGCCTCTGAA GTGTGTCAGC
           3181 AAATCCCATC CACTGTTCCA GGAATTTCGC CTGTGGCAGT TCCTGTCTAA CCTGCGAATC
           3241 TACCAGAGAG AGAGGATGAT GGACGGCAGT CTGAAACTGG ACGTGGATGT CACCCGGGAG
           3301 TTCCTGCCCT CAGAAGAGGA CTACGTGAAG CTGTTTGATT GGCTGAATGA GAGAAAGGAA
           3361 ATCTCTCAGA AATTCCTGCT GGCTTATAAA CCTTTTGGGC TGAAGAAAAA CGAACAGGCA
           3421 AATTACAGAT GGAACTATGT GGAGGACAAG AGCTACCCCT GCAACGAGAC ACGGGCAGAA
           3481 ATCAAGAGCA GACTGTCCAA AGCCGGAGTG CCTGAAGAGT TTCTGACTGA AGAGAAGGAA
           3541 GAGGCCCTGT GGCACATCCT GTATTCTATT AGTGATAAGA AAGAGCTGAC TAAGGCTCTG
           3601 GGCACCTTCG CAGCCAAAAA CTGTCTGAAT GAGTCTTTCG TGGAAGTCTT TGCCAAGATC
           3661 CCCCCTTTTG AGTCAAACTA CGCTGCATAT AGCCTGAAGG CTATTAGGAA ACTGCTGGCA
           3721 CTGATGCGCA TGGGGAAGTA CTGGAATGAA CAGGCCATCG ACAGGCAGAC TCGCGATCGA
           3781 ATCGAGAAAA TTCTGACCGG AGAGTATGAC GAAACAATCC GGAGCAGAGT GAGGGAGAAG
           3841 GCAATGCTGC TGACCGATAT TAGCAGCTTC CGGGGCCTGC CTCTGTGGCT GGCCTGTTAC
           3901 ATCGTGTATG ACCGCCACTC AGAGAGCAAG GAACTGGTCA AATGGGAGAC ACCAGCCGAC
           3961 ATCGATCATT TCCTGTCCAA GTTTAAACAG AACAGCCTGC GGAACCCCAT CGTGGAACAG
           4021 GTCATTACAG AGTCCCTGCG CACTGTGCGA GACATTTGGA AGCAGGAGGG AAAAATCGAT
           4081 GAGATTCACG TGGAACTGGG CCGGGAGATG AAGAACCCTG CAAAAGAGCG CGCCCGAATT
           4141 ACAGCTCAGG TGCAGGAAAA TGAGAACACT AATCTGAGAA TCAAGGCTCT GCTGGCAGAG
           4201 TTCATGAACC CCGAATTTGA GATTGAAAAT GTGCATCCAT ACTCACCCGG CCAGCAGGAA
           4261 ATCCTGCGGA TCTACGAGGA CGGCGTGCTG AGCGGGATCG CTGAGAAGGA TCTGCCTGAC
           4321 GATATCACAG CAATTCTGAA GAAATTCCGA GAAAACGACG TGAAGAAACG GCCAACAACT
           4381 AGCGAAGTCC TGCGGTACAA ACTGTGGCTG GAGCAGCGGT ACAGATCCCC ATATACCGGA
           4441 AGAGTGATCC CCCTGGGCAA GCTGTTCACA CCTGCTTACG AGATCGAACA CGTGATTCCC
           4501 CAGAGCCGGT ATTTTGACGA TTCCATCTCT AACAAAGTGA TCTGCGAAAG TGCCGTCAAT
           4561 AAGCTGAAAG ATAACTGTCT GGGCTATGAG TTCATCAAGA AACATTCCGG GGAGATGGTG
           4621 GAACTGGGGA ATGGAGAGAC AGTGCCCGTG TTCAGCGTGG AAGAGTACGA ACGGTTCGTC
           4681 AAGGAGTCTT ACTTCGGCAA CAGTAAGAAA ATGAAGAAAC TGCTGCTGGA GGACATCCCA
           4741 GATAGCTTCA TTGAGAGACA GCTGAATGAC AGTCGATACA TCTCACGGGT GGTCACATCT
           4801 CTGCTGAGTA ACCTGGTGTG CGAAGAGGGA GAGCAGGATG GCCTGTCCAA GAATGTGATC
           4861 GTCTGTACCG GCGGGATTAC AGACAGGCTG AAGAAAGATT GGGGAGTGCA GGAAGTCTGG
           4921 AACCGCATCA TTCTGCCTCG GTTCCTGAGA CTGAATGAGA TCACCGGACG GACAGACTTT
           4981 ACAAGTACTT CAGTGAACGG CCACCTGCTG CCTGCCCTGC CACTGTACCT GCAGAAGGGC
           5041 TTTAATAAGA AAAGAATTGA CCATAGGCAC CATGCCATGG ATGCTATCGT GATTGCCTGC
           5101 GCTAACCGGA ATATCGTCAA CTACCTGAAC AATTCCTCTG CTAGAAAGAA CAGCGAAATT
           5161 AGCCGATATG ACCTGCAGCG GCTGCTGTGT GAGAAGGTGA AAACCGATGC CAACGGCAAT
           5221 TACAAGTGGA TCCTGAGGAA ACCATGGGAG ACATTCACCC AGGATGTGTA TGCCGCTCTG
           5281 ACAAACATCG TGGTCAGCTT TAAGCAGAAT CTGCGCGTGA TTAATCGAAC CACAAACTAC
           5341 TATCAGCACT ACAACGAGGC AGGGGAAAAG CGCATGATCC CTCAGACCAA AGGCGACAGA
           5401 TGGGCCATTA GAAAGCCAAT GCATAAAGAT ACTGTGTATG GCGAAGTCAA TCTGAGAAAG
           5461 GAGAAAACCC TGCCACTGAG GGACGTGGTC AAGAACCCCA GTATCGTGGT CGATAAATCA
           5521 CTGAAGAACA AGCTGTACGA GCTGCTGAAG AGCCAGTATG ACCTGAAGGC AATCGCCAAA
           5581 TACTTCGAGA CACACCAGGA CGTGTGGGCA GATGTCAACC TGAAGAAAAT TAAGGTGTAC
           5641 TACTTCACTA AGGAGACAAA CGAAAGATTC TTTGCCACTA GGAAGAGCCT GGACCCATCC
           5701 TTTGATCAGA AGAAAATCGA AGAGGAAGTG ACTGATACCG GCGTCCAGAA GATTCTGCTG
           5761 CACCATCTGC AGCAGAACAA TAACGACCCT GATATGGCCT TTTCCCCAGA CGGCATCGAT
           5821 AGGATGAACC AGAATATGAC CATTCTGAAT GACGGGAAGT GGCACCAGCC CATCTACAAA
           5881 GTGCGCACAT ATGAAAAGGC AGATAAATTC GCCGTCGGCG AGTCTGGGAA CAAGGCCAAG
           5941 AAATTTGTGG AAGCAGCCAA AGGCACCAAT CTGTTCTTTG CCGTGTACGA GAGTGTCCAG
           6001 GAGGACGAAG CTATCGGGAA GCAGGTCTGC AAACGGACAT TCGCCACTAT CCCCCTGAAC
           6061 GAAGTGATCA AGAGAAAGAA ACAGGGCCTG CCCGCTGCAC CTGAGGACCT GAACGGGAAT
           6121 CTGCCCAAGT TTGTGCTGAG CCCTAACGAT CTGGTCTACC TGCCCACCGA GGAAGAGAGG
           6181 AATAGTTCAC GCATCATTCA GCCTCTGGAC AGGGAGCGCA TCTATAAGAT GGTGAGCTCC
           6241 TCTGGGAGTC AGTGCTTCTT TATCAAAGTG TTCGTCGCCA ATTCAATTTG GGATAAGAAC
           6301 GAATACAGCA GCCTGAACAA GATGGAGAGG GCTATTACAA ACGAAATGAT CAAGGAGATT
           6361 TGTGTGCCTA TCAAAATTGA CCGCCTGGGC AATGTCAGCC TGATCCAGAT TTAAGAATTC
           6421 ggtaccgagc tcaagcttgc ggccgcctat agtgtcacct aaatcgtaac ccagctttct
           6481 tgtacaaagt ggtcgggtgg catccctgtg acccctcccc agtgcctctc ctggccctgg
           6541 aagttgccac tccagtgccc accagccttg tcctaataaa attaagttgc atcattttgt
           6601 ctgactaggt gtccttctat aatattatgg ggtggagggg ggtggtatgg agcaaggggc
           6661 aagttgggat gacaacctgt agggcctgcg gggtctattg ggaaccaagc tggagtgcag
           6721 tggcacaatc ttggctcact gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc
           6781 agcctcccga gttgttggga ttccaggcat gcatgaccag gctcagctaa tttttgtttt
           6841 tttggtagag acggggtttc accatattgg ccaggctggt ctccaactcc taatctcagg
           6901 tgatctaccc accttggcct cccaaattgc tgggattaca ggcgtgaacc actgctccct
           6961 tccctgtcct tctagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgt
           7021 ctagaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
           7081 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
           7141 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
           7201 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
           7261 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
           7321 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
           7381 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
           7441 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
           7501 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
           7561 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
           7621 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
           7681 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
           7741 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
           7801 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
           7861 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
           7921 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
           7981 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
           8041 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
           8101 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
           8161 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
           8221 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
           8281 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
           8341 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
           8401 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
           8461 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
           8521 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
           8581 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
           8641 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
           8701 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
           8761 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
           8821 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
           8881 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
           8941 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
           9001 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgtt catgaagccc
           9061 cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg
           9121 aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag
           9181 aatgagtatt tggtttagag tttggcaaca tatgcccata tgtaactagc ataacccctt
           9241 ggggcctcta aacgggtctt gaggggtttt ttgctgaaag catgcggagg aaattctcct
           9301 tgaagtttcc ctggtgttca aagtaaagga gtttgcacca gacgcacctc tgttcactgg
           9361 tccggcgtat taaaacacga tacattgtta ttagtacatt tattaagcgc tagattctgt
           9421 gcgttgttga tttacagaca attgttgtac gtattttaat aattcattaa atttataatc
           9481 tttagggtgg tatgttagag cgaaaatcaa atgattttca gcgtctttat atctgaattt
           9541 aaatattaaa tcctcaatag atttgtaaaa taggtttcga ttagtttcaa acaagggttg
           9601 tttttccgaa ccgatggctg gactatctaa tggattttcg ctcaacgcca caaaacttgc
           9661 caaatcttgt agcagcaatc tagctttgtc gatattcgtt tgtgttttgt tttgtaataa
           9721 aggttcgacg tcgttcaaaa tattatgcgc ttttgtattt ctttcatcac tgtcgttagt
           9781 gtacaattga ctcgacgtaa acacgttaaa tagagcttgg acatatttaa catcgggcgt
           9841 gttagcttta ttaggccgat tatcgtcgtc gtcccaaccc tcgtcgttag aagttgcttc
           9901 cgaagacgat tttgccatag ccacacgacg cctattaatt gtgtcggcta acacgtccgc
           9961 gatcaaattt gtagttgagc tttttggaat tgcgatcgca taacttcgta tagcatacat
          10021 tatacgaagt tataagctcg gaacgctgcg ctcggtcgtt cggctgcggc gagcggtatc
          10081 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
          10141 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
          10201 tttccatagg ctccgccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
          10261 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
          10321 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
          10381 gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
          10441 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
          10501 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
          10561 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
          10621 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
          10681 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
          10741 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
          10801 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
          10861 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
          10921 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
          10981 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
          11041 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
          11101 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
          11161 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
          11221 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
          11281 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
          11341 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
          11401 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
          11461 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
          11521 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
          11581 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
          11641 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
          11701 ttattgtctc atgtccgcgc gtt
          //
          
          

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