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          PX385

          價格:1000元

          聯系方式:I47-825O-882O

          買家導航

          載體基本信息

          出品公司: Addgene
          載體名稱: PX385 或 PX385 Streptococcus pasteurianus Cas9
          質粒類型: CRISPR-Cas9載體;基因敲除載體;昆蟲細胞載體
          高拷貝/低拷貝: 高拷貝
          插入位點: 限制性內切酶,多克隆位點
          啟動子: CMV
          載體大小: 10586 bp
          5' 測序引物及序列: CMV Forward:CGCAAATGGGCGGTAGGCGTG
          3' 測序引物及序列: C-CMV-24 (5'-TATTAGGACAAGGCTGGTGGGCAC-3')
          載體標簽: NLS (C ter);HA、NLS (N ter)
          載體抗性: 氨芐青霉素
          篩選標記: Neomycin
          克隆菌株: Stbl3
          宿主細胞(系): 昆蟲細胞
          備注: --
          產品目錄號: #68322
          穩定性: 穩表達
          組成型/誘導型: 組成型
          病毒/非病毒: 桿狀病毒

          載體質粒圖譜和多克隆位點信息

          PX385載體圖譜



          載體簡介

          載體序列

          LOCUS       PX385 Streptococcus pasteurianus Cas9	10586 bp 	DNA	SYN
          DEFINITION  PX385 Streptococcus pasteurianus Cas9
          ACCESSION   
          KEYWORDS    
          SOURCE      
            ORGANISM  other sequences; artificial sequences; vectors.
          FEATURES             Location/Qualifiers
               source          1..10586
                               /organism="PX385 Streptococcus pasteurianus Cas9"
                               /mol_type="other DNA"
               misc_feature    47..1001
                               /label="Ac_5_flank"
               CDS             complement(343..948)
                               /label="ORF frame 3"
                               /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                               VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                               PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                               LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
               promoter        1023..1587
                               /label="CMV_immearly_promoter"
                               /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                               VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                               PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                               LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
               misc_feature    1090..1377
                               /label="CAG_enhancer"
                               /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                               VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                               PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                               LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
               misc_feature    1544..1564
                               /label="CMV_fwd_primer"
                               /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                               VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                               PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                               LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
               promoter        1615..1633
                               /label="T7_promoter"
                               /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                               VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                               PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                               LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
               misc_feature    1633..1656
                               /label="lacO"
                               /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                               VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                               PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                               LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
               misc_feature    1772..1796
                               /label="attB1"
                               /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                               VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                               PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                               LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
               CDS             1822..5277
                               /label="ORF frame 1"
                               /translation="MYPYDVPDYASPKKKRKVEASMTNGKILGLDIGIASVGVGIIEA
                               KTGKVVHANSRLFSAANAENNAERRGFRGSRRLNRRKKHRVKRVRDLFEKYGIVTDFR
                               NLNLNPYELRVKGLTEQLKNEELFAALRTISKRRGISYLDDAEDDSTGSTDYAKSIDE
                               NRRLLKNKTPGQIQLERLEKYGQLRGNFTVYDENGEAHRLINVFSTSDYEKEARKILE
                               TQADYNKKITAEFIDDYVEILTQKRKYYHGPGNEKSRTDYGRFRTDGTTLENIFGILI
                               GKCNFYPDEYRASKASYTAQEYNFLNDLNNLKVSTETGKLSTEQKESLVEFAKNTATL
                               GPAKLLKEIAKILDCKVDEIKGYREDDKGKPDLHTFEPYRKLKFNLESINIDDLSREV
                               IDKLADILTLNTEREGIEDAIKRNLPNQFTEEQISEIIKVRKSQSTAFNKGWHSFSAK
                               LMNELIPELYATSDEQMTILTRLEKFKVNKKSSKNTKTIDEKEVTDEIYNPVVAKSVR
                               QTIKIINAAVKKYGDFDKIVIEMPRDKNADDEKKFIDKRNKENKKEKDDALKRAAYLY
                               NSSDKLPDEVFHGNKQLETKIRLWYQQGERCLYSGKPISIQELVHNSNNFEIDHILPL
                               SLSFDDSLANKVLVYAWTNQEKGQKTPYQVIDSMDAAWSFREMKDYVLKQKGLGKKKR
                               DYLLTTENIDKIEVKKKFIERNLVDTRYASRVVLNSLQSALRELGKDTKVSVVRGQFT
                               SQLRRKWKIDKSRETYHHHAVDALIIAASSQLKLWEKQDNPMFVDYGKNQVVDKQTGE
                               ILSVSDDEYKELVFQPPYQGFVNTISSKGFEDEILFSYQVDSKYNRKVSDATIYSTRK
                               AKIGKDKKEETYVLGKIKDIYSQNGFDTFIKKYNKDKTQFLMYQKDSLTWENVIEVIL
                               RDYPTTKKSEDGKNDVKCNPFEEYRRENGLICKYSKKGKGTPIKSLKYYDKKLGNCID
                               ITPEESRNKVILQSINPWRADVYFNPETLKYELMGLKYSDLSFEKGTGNYHISQEKYD
                               AIKEKEGIGKKSEFKFTLYRNDLILIKDIASGEQEIYRFLSRTMPNVNHYVELKPYDK
                               EKFDNVQELVEALGEADKVGRCIKGLNKPNISIYKVRTDVLGNKYFVKKKGDKPKLDF
                               KNNKK*"
               misc_feature    1825..1851
                               /label="HA"
                               /translation="MYPYDVPDYASPKKKRKVEASMTNGKILGLDIGIASVGVGIIEA
                               KTGKVVHANSRLFSAANAENNAERRGFRGSRRLNRRKKHRVKRVRDLFEKYGIVTDFR
                               NLNLNPYELRVKGLTEQLKNEELFAALRTISKRRGISYLDDAEDDSTGSTDYAKSIDE
                               NRRLLKNKTPGQIQLERLEKYGQLRGNFTVYDENGEAHRLINVFSTSDYEKEARKILE
                               TQADYNKKITAEFIDDYVEILTQKRKYYHGPGNEKSRTDYGRFRTDGTTLENIFGILI
                               GKCNFYPDEYRASKASYTAQEYNFLNDLNNLKVSTETGKLSTEQKESLVEFAKNTATL
                               GPAKLLKEIAKILDCKVDEIKGYREDDKGKPDLHTFEPYRKLKFNLESINIDDLSREV
                               IDKLADILTLNTEREGIEDAIKRNLPNQFTEEQISEIIKVRKSQSTAFNKGWHSFSAK
                               LMNELIPELYATSDEQMTILTRLEKFKVNKKSSKNTKTIDEKEVTDEIYNPVVAKSVR
                               QTIKIINAAVKKYGDFDKIVIEMPRDKNADDEKKFIDKRNKENKKEKDDALKRAAYLY
                               NSSDKLPDEVFHGNKQLETKIRLWYQQGERCLYSGKPISIQELVHNSNNFEIDHILPL
                               SLSFDDSLANKVLVYAWTNQEKGQKTPYQVIDSMDAAWSFREMKDYVLKQKGLGKKKR
                               DYLLTTENIDKIEVKKKFIERNLVDTRYASRVVLNSLQSALRELGKDTKVSVVRGQFT
                               SQLRRKWKIDKSRETYHHHAVDALIIAASSQLKLWEKQDNPMFVDYGKNQVVDKQTGE
                               ILSVSDDEYKELVFQPPYQGFVNTISSKGFEDEILFSYQVDSKYNRKVSDATIYSTRK
                               AKIGKDKKEETYVLGKIKDIYSQNGFDTFIKKYNKDKTQFLMYQKDSLTWENVIEVIL
                               RDYPTTKKSEDGKNDVKCNPFEEYRRENGLICKYSKKGKGTPIKSLKYYDKKLGNCID
                               ITPEESRNKVILQSINPWRADVYFNPETLKYELMGLKYSDLSFEKGTGNYHISQEKYD
                               AIKEKEGIGKKSEFKFTLYRNDLILIKDIASGEQEIYRFLSRTMPNVNHYVELKPYDK
                               EKFDNVQELVEALGEADKVGRCIKGLNKPNISIYKVRTDVLGNKYFVKKKGDKPKLDF
                               KNNKK*"
               misc_feature    1855..1875
                               /label="SV40 NLS"
                               /translation="MYPYDVPDYASPKKKRKVEASMTNGKILGLDIGIASVGVGIIEA
                               KTGKVVHANSRLFSAANAENNAERRGFRGSRRLNRRKKHRVKRVRDLFEKYGIVTDFR
                               NLNLNPYELRVKGLTEQLKNEELFAALRTISKRRGISYLDDAEDDSTGSTDYAKSIDE
                               NRRLLKNKTPGQIQLERLEKYGQLRGNFTVYDENGEAHRLINVFSTSDYEKEARKILE
                               TQADYNKKITAEFIDDYVEILTQKRKYYHGPGNEKSRTDYGRFRTDGTTLENIFGILI
                               GKCNFYPDEYRASKASYTAQEYNFLNDLNNLKVSTETGKLSTEQKESLVEFAKNTATL
                               GPAKLLKEIAKILDCKVDEIKGYREDDKGKPDLHTFEPYRKLKFNLESINIDDLSREV
                               IDKLADILTLNTEREGIEDAIKRNLPNQFTEEQISEIIKVRKSQSTAFNKGWHSFSAK
                               LMNELIPELYATSDEQMTILTRLEKFKVNKKSSKNTKTIDEKEVTDEIYNPVVAKSVR
                               QTIKIINAAVKKYGDFDKIVIEMPRDKNADDEKKFIDKRNKENKKEKDDALKRAAYLY
                               NSSDKLPDEVFHGNKQLETKIRLWYQQGERCLYSGKPISIQELVHNSNNFEIDHILPL
                               SLSFDDSLANKVLVYAWTNQEKGQKTPYQVIDSMDAAWSFREMKDYVLKQKGLGKKKR
                               DYLLTTENIDKIEVKKKFIERNLVDTRYASRVVLNSLQSALRELGKDTKVSVVRGQFT
                               SQLRRKWKIDKSRETYHHHAVDALIIAASSQLKLWEKQDNPMFVDYGKNQVVDKQTGE
                               ILSVSDDEYKELVFQPPYQGFVNTISSKGFEDEILFSYQVDSKYNRKVSDATIYSTRK
                               AKIGKDKKEETYVLGKIKDIYSQNGFDTFIKKYNKDKTQFLMYQKDSLTWENVIEVIL
                               RDYPTTKKSEDGKNDVKCNPFEEYRRENGLICKYSKKGKGTPIKSLKYYDKKLGNCID
                               ITPEESRNKVILQSINPWRADVYFNPETLKYELMGLKYSDLSFEKGTGNYHISQEKYD
                               AIKEKEGIGKKSEFKFTLYRNDLILIKDIASGEQEIYRFLSRTMPNVNHYVELKPYDK
                               EKFDNVQELVEALGEADKVGRCIKGLNKPNISIYKVRTDVLGNKYFVKKKGDKPKLDF
                               KNNKK*"
               misc_feature    1861..1875
                               /label="NLS"
                               /translation="MYPYDVPDYASPKKKRKVEASMTNGKILGLDIGIASVGVGIIEA
                               KTGKVVHANSRLFSAANAENNAERRGFRGSRRLNRRKKHRVKRVRDLFEKYGIVTDFR
                               NLNLNPYELRVKGLTEQLKNEELFAALRTISKRRGISYLDDAEDDSTGSTDYAKSIDE
                               NRRLLKNKTPGQIQLERLEKYGQLRGNFTVYDENGEAHRLINVFSTSDYEKEARKILE
                               TQADYNKKITAEFIDDYVEILTQKRKYYHGPGNEKSRTDYGRFRTDGTTLENIFGILI
                               GKCNFYPDEYRASKASYTAQEYNFLNDLNNLKVSTETGKLSTEQKESLVEFAKNTATL
                               GPAKLLKEIAKILDCKVDEIKGYREDDKGKPDLHTFEPYRKLKFNLESINIDDLSREV
                               IDKLADILTLNTEREGIEDAIKRNLPNQFTEEQISEIIKVRKSQSTAFNKGWHSFSAK
                               LMNELIPELYATSDEQMTILTRLEKFKVNKKSSKNTKTIDEKEVTDEIYNPVVAKSVR
                               QTIKIINAAVKKYGDFDKIVIEMPRDKNADDEKKFIDKRNKENKKEKDDALKRAAYLY
                               NSSDKLPDEVFHGNKQLETKIRLWYQQGERCLYSGKPISIQELVHNSNNFEIDHILPL
                               SLSFDDSLANKVLVYAWTNQEKGQKTPYQVIDSMDAAWSFREMKDYVLKQKGLGKKKR
                               DYLLTTENIDKIEVKKKFIERNLVDTRYASRVVLNSLQSALRELGKDTKVSVVRGQFT
                               SQLRRKWKIDKSRETYHHHAVDALIIAASSQLKLWEKQDNPMFVDYGKNQVVDKQTGE
                               ILSVSDDEYKELVFQPPYQGFVNTISSKGFEDEILFSYQVDSKYNRKVSDATIYSTRK
                               AKIGKDKKEETYVLGKIKDIYSQNGFDTFIKKYNKDKTQFLMYQKDSLTWENVIEVIL
                               RDYPTTKKSEDGKNDVKCNPFEEYRRENGLICKYSKKGKGTPIKSLKYYDKKLGNCID
                               ITPEESRNKVILQSINPWRADVYFNPETLKYELMGLKYSDLSFEKGTGNYHISQEKYD
                               AIKEKEGIGKKSEFKFTLYRNDLILIKDIASGEQEIYRFLSRTMPNVNHYVELKPYDK
                               EKFDNVQELVEALGEADKVGRCIKGLNKPNISIYKVRTDVLGNKYFVKKKGDKPKLDF
                               KNNKK*"
               misc_feature    complement(5310..5327)
                               /label="Sp6_primer"
                               /translation="MYPYDVPDYASPKKKRKVEASMTNGKILGLDIGIASVGVGIIEA
                               KTGKVVHANSRLFSAANAENNAERRGFRGSRRLNRRKKHRVKRVRDLFEKYGIVTDFR
                               NLNLNPYELRVKGLTEQLKNEELFAALRTISKRRGISYLDDAEDDSTGSTDYAKSIDE
                               NRRLLKNKTPGQIQLERLEKYGQLRGNFTVYDENGEAHRLINVFSTSDYEKEARKILE
                               TQADYNKKITAEFIDDYVEILTQKRKYYHGPGNEKSRTDYGRFRTDGTTLENIFGILI
                               GKCNFYPDEYRASKASYTAQEYNFLNDLNNLKVSTETGKLSTEQKESLVEFAKNTATL
                               GPAKLLKEIAKILDCKVDEIKGYREDDKGKPDLHTFEPYRKLKFNLESINIDDLSREV
                               IDKLADILTLNTEREGIEDAIKRNLPNQFTEEQISEIIKVRKSQSTAFNKGWHSFSAK
                               LMNELIPELYATSDEQMTILTRLEKFKVNKKSSKNTKTIDEKEVTDEIYNPVVAKSVR
                               QTIKIINAAVKKYGDFDKIVIEMPRDKNADDEKKFIDKRNKENKKEKDDALKRAAYLY
                               NSSDKLPDEVFHGNKQLETKIRLWYQQGERCLYSGKPISIQELVHNSNNFEIDHILPL
                               SLSFDDSLANKVLVYAWTNQEKGQKTPYQVIDSMDAAWSFREMKDYVLKQKGLGKKKR
                               DYLLTTENIDKIEVKKKFIERNLVDTRYASRVVLNSLQSALRELGKDTKVSVVRGQFT
                               SQLRRKWKIDKSRETYHHHAVDALIIAASSQLKLWEKQDNPMFVDYGKNQVVDKQTGE
                               ILSVSDDEYKELVFQPPYQGFVNTISSKGFEDEILFSYQVDSKYNRKVSDATIYSTRK
                               AKIGKDKKEETYVLGKIKDIYSQNGFDTFIKKYNKDKTQFLMYQKDSLTWENVIEVIL
                               RDYPTTKKSEDGKNDVKCNPFEEYRRENGLICKYSKKGKGTPIKSLKYYDKKLGNCID
                               ITPEESRNKVILQSINPWRADVYFNPETLKYELMGLKYSDLSFEKGTGNYHISQEKYD
                               AIKEKEGIGKKSEFKFTLYRNDLILIKDIASGEQEIYRFLSRTMPNVNHYVELKPYDK
                               EKFDNVQELVEALGEADKVGRCIKGLNKPNISIYKVRTDVLGNKYFVKKKGDKPKLDF
                               KNNKK*"
               misc_feature    5332..5356
                               /label="attB2"
                               /translation="MYPYDVPDYASPKKKRKVEASMTNGKILGLDIGIASVGVGIIEA
                               KTGKVVHANSRLFSAANAENNAERRGFRGSRRLNRRKKHRVKRVRDLFEKYGIVTDFR
                               NLNLNPYELRVKGLTEQLKNEELFAALRTISKRRGISYLDDAEDDSTGSTDYAKSIDE
                               NRRLLKNKTPGQIQLERLEKYGQLRGNFTVYDENGEAHRLINVFSTSDYEKEARKILE
                               TQADYNKKITAEFIDDYVEILTQKRKYYHGPGNEKSRTDYGRFRTDGTTLENIFGILI
                               GKCNFYPDEYRASKASYTAQEYNFLNDLNNLKVSTETGKLSTEQKESLVEFAKNTATL
                               GPAKLLKEIAKILDCKVDEIKGYREDDKGKPDLHTFEPYRKLKFNLESINIDDLSREV
                               IDKLADILTLNTEREGIEDAIKRNLPNQFTEEQISEIIKVRKSQSTAFNKGWHSFSAK
                               LMNELIPELYATSDEQMTILTRLEKFKVNKKSSKNTKTIDEKEVTDEIYNPVVAKSVR
                               QTIKIINAAVKKYGDFDKIVIEMPRDKNADDEKKFIDKRNKENKKEKDDALKRAAYLY
                               NSSDKLPDEVFHGNKQLETKIRLWYQQGERCLYSGKPISIQELVHNSNNFEIDHILPL
                               SLSFDDSLANKVLVYAWTNQEKGQKTPYQVIDSMDAAWSFREMKDYVLKQKGLGKKKR
                               DYLLTTENIDKIEVKKKFIERNLVDTRYASRVVLNSLQSALRELGKDTKVSVVRGQFT
                               SQLRRKWKIDKSRETYHHHAVDALIIAASSQLKLWEKQDNPMFVDYGKNQVVDKQTGE
                               ILSVSDDEYKELVFQPPYQGFVNTISSKGFEDEILFSYQVDSKYNRKVSDATIYSTRK
                               AKIGKDKKEETYVLGKIKDIYSQNGFDTFIKKYNKDKTQFLMYQKDSLTWENVIEVIL
                               RDYPTTKKSEDGKNDVKCNPFEEYRRENGLICKYSKKGKGTPIKSLKYYDKKLGNCID
                               ITPEESRNKVILQSINPWRADVYFNPETLKYELMGLKYSDLSFEKGTGNYHISQEKYD
                               AIKEKEGIGKKSEFKFTLYRNDLILIKDIASGEQEIYRFLSRTMPNVNHYVELKPYDK
                               EKFDNVQELVEALGEADKVGRCIKGLNKPNISIYKVRTDVLGNKYFVKKKGDKPKLDF
                               KNNKK*"
               terminator      5368..5834
                               /label="hGH_PA_terminator"
                               /translation="MYPYDVPDYASPKKKRKVEASMTNGKILGLDIGIASVGVGIIEA
                               KTGKVVHANSRLFSAANAENNAERRGFRGSRRLNRRKKHRVKRVRDLFEKYGIVTDFR
                               NLNLNPYELRVKGLTEQLKNEELFAALRTISKRRGISYLDDAEDDSTGSTDYAKSIDE
                               NRRLLKNKTPGQIQLERLEKYGQLRGNFTVYDENGEAHRLINVFSTSDYEKEARKILE
                               TQADYNKKITAEFIDDYVEILTQKRKYYHGPGNEKSRTDYGRFRTDGTTLENIFGILI
                               GKCNFYPDEYRASKASYTAQEYNFLNDLNNLKVSTETGKLSTEQKESLVEFAKNTATL
                               GPAKLLKEIAKILDCKVDEIKGYREDDKGKPDLHTFEPYRKLKFNLESINIDDLSREV
                               IDKLADILTLNTEREGIEDAIKRNLPNQFTEEQISEIIKVRKSQSTAFNKGWHSFSAK
                               LMNELIPELYATSDEQMTILTRLEKFKVNKKSSKNTKTIDEKEVTDEIYNPVVAKSVR
                               QTIKIINAAVKKYGDFDKIVIEMPRDKNADDEKKFIDKRNKENKKEKDDALKRAAYLY
                               NSSDKLPDEVFHGNKQLETKIRLWYQQGERCLYSGKPISIQELVHNSNNFEIDHILPL
                               SLSFDDSLANKVLVYAWTNQEKGQKTPYQVIDSMDAAWSFREMKDYVLKQKGLGKKKR
                               DYLLTTENIDKIEVKKKFIERNLVDTRYASRVVLNSLQSALRELGKDTKVSVVRGQFT
                               SQLRRKWKIDKSRETYHHHAVDALIIAASSQLKLWEKQDNPMFVDYGKNQVVDKQTGE
                               ILSVSDDEYKELVFQPPYQGFVNTISSKGFEDEILFSYQVDSKYNRKVSDATIYSTRK
                               AKIGKDKKEETYVLGKIKDIYSQNGFDTFIKKYNKDKTQFLMYQKDSLTWENVIEVIL
                               RDYPTTKKSEDGKNDVKCNPFEEYRRENGLICKYSKKGKGTPIKSLKYYDKKLGNCID
                               ITPEESRNKVILQSINPWRADVYFNPETLKYELMGLKYSDLSFEKGTGNYHISQEKYD
                               AIKEKEGIGKKSEFKFTLYRNDLILIKDIASGEQEIYRFLSRTMPNVNHYVELKPYDK
                               EKFDNVQELVEALGEADKVGRCIKGLNKPNISIYKVRTDVLGNKYFVKKKGDKPKLDF
                               KNNKK*"
               terminator      5840..5875
                               /label="T7_terminator"
                               /translation="MYPYDVPDYASPKKKRKVEASMTNGKILGLDIGIASVGVGIIEA
                               KTGKVVHANSRLFSAANAENNAERRGFRGSRRLNRRKKHRVKRVRDLFEKYGIVTDFR
                               NLNLNPYELRVKGLTEQLKNEELFAALRTISKRRGISYLDDAEDDSTGSTDYAKSIDE
                               NRRLLKNKTPGQIQLERLEKYGQLRGNFTVYDENGEAHRLINVFSTSDYEKEARKILE
                               TQADYNKKITAEFIDDYVEILTQKRKYYHGPGNEKSRTDYGRFRTDGTTLENIFGILI
                               GKCNFYPDEYRASKASYTAQEYNFLNDLNNLKVSTETGKLSTEQKESLVEFAKNTATL
                               GPAKLLKEIAKILDCKVDEIKGYREDDKGKPDLHTFEPYRKLKFNLESINIDDLSREV
                               IDKLADILTLNTEREGIEDAIKRNLPNQFTEEQISEIIKVRKSQSTAFNKGWHSFSAK
                               LMNELIPELYATSDEQMTILTRLEKFKVNKKSSKNTKTIDEKEVTDEIYNPVVAKSVR
                               QTIKIINAAVKKYGDFDKIVIEMPRDKNADDEKKFIDKRNKENKKEKDDALKRAAYLY
                               NSSDKLPDEVFHGNKQLETKIRLWYQQGERCLYSGKPISIQELVHNSNNFEIDHILPL
                               SLSFDDSLANKVLVYAWTNQEKGQKTPYQVIDSMDAAWSFREMKDYVLKQKGLGKKKR
                               DYLLTTENIDKIEVKKKFIERNLVDTRYASRVVLNSLQSALRELGKDTKVSVVRGQFT
                               SQLRRKWKIDKSRETYHHHAVDALIIAASSQLKLWEKQDNPMFVDYGKNQVVDKQTGE
                               ILSVSDDEYKELVFQPPYQGFVNTISSKGFEDEILFSYQVDSKYNRKVSDATIYSTRK
                               AKIGKDKKEETYVLGKIKDIYSQNGFDTFIKKYNKDKTQFLMYQKDSLTWENVIEVIL
                               RDYPTTKKSEDGKNDVKCNPFEEYRRENGLICKYSKKGKGTPIKSLKYYDKKLGNCID
                               ITPEESRNKVILQSINPWRADVYFNPETLKYELMGLKYSDLSFEKGTGNYHISQEKYD
                               AIKEKEGIGKKSEFKFTLYRNDLILIKDIASGEQEIYRFLSRTMPNVNHYVELKPYDK
                               EKFDNVQELVEALGEADKVGRCIKGLNKPNISIYKVRTDVLGNKYFVKKKGDKPKLDF
                               KNNKK*"
               rep_origin      5992..6298
                               /label="f1_origin"
                               /translation="MYPYDVPDYASPKKKRKVEASMTNGKILGLDIGIASVGVGIIEA
                               KTGKVVHANSRLFSAANAENNAERRGFRGSRRLNRRKKHRVKRVRDLFEKYGIVTDFR
                               NLNLNPYELRVKGLTEQLKNEELFAALRTISKRRGISYLDDAEDDSTGSTDYAKSIDE
                               NRRLLKNKTPGQIQLERLEKYGQLRGNFTVYDENGEAHRLINVFSTSDYEKEARKILE
                               TQADYNKKITAEFIDDYVEILTQKRKYYHGPGNEKSRTDYGRFRTDGTTLENIFGILI
                               GKCNFYPDEYRASKASYTAQEYNFLNDLNNLKVSTETGKLSTEQKESLVEFAKNTATL
                               GPAKLLKEIAKILDCKVDEIKGYREDDKGKPDLHTFEPYRKLKFNLESINIDDLSREV
                               IDKLADILTLNTEREGIEDAIKRNLPNQFTEEQISEIIKVRKSQSTAFNKGWHSFSAK
                               LMNELIPELYATSDEQMTILTRLEKFKVNKKSSKNTKTIDEKEVTDEIYNPVVAKSVR
                               QTIKIINAAVKKYGDFDKIVIEMPRDKNADDEKKFIDKRNKENKKEKDDALKRAAYLY
                               NSSDKLPDEVFHGNKQLETKIRLWYQQGERCLYSGKPISIQELVHNSNNFEIDHILPL
                               SLSFDDSLANKVLVYAWTNQEKGQKTPYQVIDSMDAAWSFREMKDYVLKQKGLGKKKR
                               DYLLTTENIDKIEVKKKFIERNLVDTRYASRVVLNSLQSALRELGKDTKVSVVRGQFT
                               SQLRRKWKIDKSRETYHHHAVDALIIAASSQLKLWEKQDNPMFVDYGKNQVVDKQTGE
                               ILSVSDDEYKELVFQPPYQGFVNTISSKGFEDEILFSYQVDSKYNRKVSDATIYSTRK
                               AKIGKDKKEETYVLGKIKDIYSQNGFDTFIKKYNKDKTQFLMYQKDSLTWENVIEVIL
                               RDYPTTKKSEDGKNDVKCNPFEEYRRENGLICKYSKKGKGTPIKSLKYYDKKLGNCID
                               ITPEESRNKVILQSINPWRADVYFNPETLKYELMGLKYSDLSFEKGTGNYHISQEKYD
                               AIKEKEGIGKKSEFKFTLYRNDLILIKDIASGEQEIYRFLSRTMPNVNHYVELKPYDK
                               EKFDNVQELVEALGEADKVGRCIKGLNKPNISIYKVRTDVLGNKYFVKKKGDKPKLDF
                               KNNKK*"
               misc_feature    complement(6412..6432)
                               /label="pBABE_3_primer"
                               /translation="MYPYDVPDYASPKKKRKVEASMTNGKILGLDIGIASVGVGIIEA
                               KTGKVVHANSRLFSAANAENNAERRGFRGSRRLNRRKKHRVKRVRDLFEKYGIVTDFR
                               NLNLNPYELRVKGLTEQLKNEELFAALRTISKRRGISYLDDAEDDSTGSTDYAKSIDE
                               NRRLLKNKTPGQIQLERLEKYGQLRGNFTVYDENGEAHRLINVFSTSDYEKEARKILE
                               TQADYNKKITAEFIDDYVEILTQKRKYYHGPGNEKSRTDYGRFRTDGTTLENIFGILI
                               GKCNFYPDEYRASKASYTAQEYNFLNDLNNLKVSTETGKLSTEQKESLVEFAKNTATL
                               GPAKLLKEIAKILDCKVDEIKGYREDDKGKPDLHTFEPYRKLKFNLESINIDDLSREV
                               IDKLADILTLNTEREGIEDAIKRNLPNQFTEEQISEIIKVRKSQSTAFNKGWHSFSAK
                               LMNELIPELYATSDEQMTILTRLEKFKVNKKSSKNTKTIDEKEVTDEIYNPVVAKSVR
                               QTIKIINAAVKKYGDFDKIVIEMPRDKNADDEKKFIDKRNKENKKEKDDALKRAAYLY
                               NSSDKLPDEVFHGNKQLETKIRLWYQQGERCLYSGKPISIQELVHNSNNFEIDHILPL
                               SLSFDDSLANKVLVYAWTNQEKGQKTPYQVIDSMDAAWSFREMKDYVLKQKGLGKKKR
                               DYLLTTENIDKIEVKKKFIERNLVDTRYASRVVLNSLQSALRELGKDTKVSVVRGQFT
                               SQLRRKWKIDKSRETYHHHAVDALIIAASSQLKLWEKQDNPMFVDYGKNQVVDKQTGE
                               ILSVSDDEYKELVFQPPYQGFVNTISSKGFEDEILFSYQVDSKYNRKVSDATIYSTRK
                               AKIGKDKKEETYVLGKIKDIYSQNGFDTFIKKYNKDKTQFLMYQKDSLTWENVIEVIL
                               RDYPTTKKSEDGKNDVKCNPFEEYRRENGLICKYSKKGKGTPIKSLKYYDKKLGNCID
                               ITPEESRNKVILQSINPWRADVYFNPETLKYELMGLKYSDLSFEKGTGNYHISQEKYD
                               AIKEKEGIGKKSEFKFTLYRNDLILIKDIASGEQEIYRFLSRTMPNVNHYVELKPYDK
                               EKFDNVQELVEALGEADKVGRCIKGLNKPNISIYKVRTDVLGNKYFVKKKGDKPKLDF
                               KNNKK*"
               misc_feature    complement(6418..6633)
                               /label="SV40_enhancer"
                               /translation="MYPYDVPDYASPKKKRKVEASMTNGKILGLDIGIASVGVGIIEA
                               KTGKVVHANSRLFSAANAENNAERRGFRGSRRLNRRKKHRVKRVRDLFEKYGIVTDFR
                               NLNLNPYELRVKGLTEQLKNEELFAALRTISKRRGISYLDDAEDDSTGSTDYAKSIDE
                               NRRLLKNKTPGQIQLERLEKYGQLRGNFTVYDENGEAHRLINVFSTSDYEKEARKILE
                               TQADYNKKITAEFIDDYVEILTQKRKYYHGPGNEKSRTDYGRFRTDGTTLENIFGILI
                               GKCNFYPDEYRASKASYTAQEYNFLNDLNNLKVSTETGKLSTEQKESLVEFAKNTATL
                               GPAKLLKEIAKILDCKVDEIKGYREDDKGKPDLHTFEPYRKLKFNLESINIDDLSREV
                               IDKLADILTLNTEREGIEDAIKRNLPNQFTEEQISEIIKVRKSQSTAFNKGWHSFSAK
                               LMNELIPELYATSDEQMTILTRLEKFKVNKKSSKNTKTIDEKEVTDEIYNPVVAKSVR
                               QTIKIINAAVKKYGDFDKIVIEMPRDKNADDEKKFIDKRNKENKKEKDDALKRAAYLY
                               NSSDKLPDEVFHGNKQLETKIRLWYQQGERCLYSGKPISIQELVHNSNNFEIDHILPL
                               SLSFDDSLANKVLVYAWTNQEKGQKTPYQVIDSMDAAWSFREMKDYVLKQKGLGKKKR
                               DYLLTTENIDKIEVKKKFIERNLVDTRYASRVVLNSLQSALRELGKDTKVSVVRGQFT
                               SQLRRKWKIDKSRETYHHHAVDALIIAASSQLKLWEKQDNPMFVDYGKNQVVDKQTGE
                               ILSVSDDEYKELVFQPPYQGFVNTISSKGFEDEILFSYQVDSKYNRKVSDATIYSTRK
                               AKIGKDKKEETYVLGKIKDIYSQNGFDTFIKKYNKDKTQFLMYQKDSLTWENVIEVIL
                               RDYPTTKKSEDGKNDVKCNPFEEYRRENGLICKYSKKGKGTPIKSLKYYDKKLGNCID
                               ITPEESRNKVILQSINPWRADVYFNPETLKYELMGLKYSDLSFEKGTGNYHISQEKYD
                               AIKEKEGIGKKSEFKFTLYRNDLILIKDIASGEQEIYRFLSRTMPNVNHYVELKPYDK
                               EKFDNVQELVEALGEADKVGRCIKGLNKPNISIYKVRTDVLGNKYFVKKKGDKPKLDF
                               KNNKK*"
               promoter        6430..6698
                               /label="SV40_promoter"
                               /translation="MYPYDVPDYASPKKKRKVEASMTNGKILGLDIGIASVGVGIIEA
                               KTGKVVHANSRLFSAANAENNAERRGFRGSRRLNRRKKHRVKRVRDLFEKYGIVTDFR
                               NLNLNPYELRVKGLTEQLKNEELFAALRTISKRRGISYLDDAEDDSTGSTDYAKSIDE
                               NRRLLKNKTPGQIQLERLEKYGQLRGNFTVYDENGEAHRLINVFSTSDYEKEARKILE
                               TQADYNKKITAEFIDDYVEILTQKRKYYHGPGNEKSRTDYGRFRTDGTTLENIFGILI
                               GKCNFYPDEYRASKASYTAQEYNFLNDLNNLKVSTETGKLSTEQKESLVEFAKNTATL
                               GPAKLLKEIAKILDCKVDEIKGYREDDKGKPDLHTFEPYRKLKFNLESINIDDLSREV
                               IDKLADILTLNTEREGIEDAIKRNLPNQFTEEQISEIIKVRKSQSTAFNKGWHSFSAK
                               LMNELIPELYATSDEQMTILTRLEKFKVNKKSSKNTKTIDEKEVTDEIYNPVVAKSVR
                               QTIKIINAAVKKYGDFDKIVIEMPRDKNADDEKKFIDKRNKENKKEKDDALKRAAYLY
                               NSSDKLPDEVFHGNKQLETKIRLWYQQGERCLYSGKPISIQELVHNSNNFEIDHILPL
                               SLSFDDSLANKVLVYAWTNQEKGQKTPYQVIDSMDAAWSFREMKDYVLKQKGLGKKKR
                               DYLLTTENIDKIEVKKKFIERNLVDTRYASRVVLNSLQSALRELGKDTKVSVVRGQFT
                               SQLRRKWKIDKSRETYHHHAVDALIIAASSQLKLWEKQDNPMFVDYGKNQVVDKQTGE
                               ILSVSDDEYKELVFQPPYQGFVNTISSKGFEDEILFSYQVDSKYNRKVSDATIYSTRK
                               AKIGKDKKEETYVLGKIKDIYSQNGFDTFIKKYNKDKTQFLMYQKDSLTWENVIEVIL
                               RDYPTTKKSEDGKNDVKCNPFEEYRRENGLICKYSKKGKGTPIKSLKYYDKKLGNCID
                               ITPEESRNKVILQSINPWRADVYFNPETLKYELMGLKYSDLSFEKGTGNYHISQEKYD
                               AIKEKEGIGKKSEFKFTLYRNDLILIKDIASGEQEIYRFLSRTMPNVNHYVELKPYDK
                               EKFDNVQELVEALGEADKVGRCIKGLNKPNISIYKVRTDVLGNKYFVKKKGDKPKLDF
                               KNNKK*"
               rep_origin      6597..6674
                               /label="SV40_origin"
                               /translation="MYPYDVPDYASPKKKRKVEASMTNGKILGLDIGIASVGVGIIEA
                               KTGKVVHANSRLFSAANAENNAERRGFRGSRRLNRRKKHRVKRVRDLFEKYGIVTDFR
                               NLNLNPYELRVKGLTEQLKNEELFAALRTISKRRGISYLDDAEDDSTGSTDYAKSIDE
                               NRRLLKNKTPGQIQLERLEKYGQLRGNFTVYDENGEAHRLINVFSTSDYEKEARKILE
                               TQADYNKKITAEFIDDYVEILTQKRKYYHGPGNEKSRTDYGRFRTDGTTLENIFGILI
                               GKCNFYPDEYRASKASYTAQEYNFLNDLNNLKVSTETGKLSTEQKESLVEFAKNTATL
                               GPAKLLKEIAKILDCKVDEIKGYREDDKGKPDLHTFEPYRKLKFNLESINIDDLSREV
                               IDKLADILTLNTEREGIEDAIKRNLPNQFTEEQISEIIKVRKSQSTAFNKGWHSFSAK
                               LMNELIPELYATSDEQMTILTRLEKFKVNKKSSKNTKTIDEKEVTDEIYNPVVAKSVR
                               QTIKIINAAVKKYGDFDKIVIEMPRDKNADDEKKFIDKRNKENKKEKDDALKRAAYLY
                               NSSDKLPDEVFHGNKQLETKIRLWYQQGERCLYSGKPISIQELVHNSNNFEIDHILPL
                               SLSFDDSLANKVLVYAWTNQEKGQKTPYQVIDSMDAAWSFREMKDYVLKQKGLGKKKR
                               DYLLTTENIDKIEVKKKFIERNLVDTRYASRVVLNSLQSALRELGKDTKVSVVRGQFT
                               SQLRRKWKIDKSRETYHHHAVDALIIAASSQLKLWEKQDNPMFVDYGKNQVVDKQTGE
                               ILSVSDDEYKELVFQPPYQGFVNTISSKGFEDEILFSYQVDSKYNRKVSDATIYSTRK
                               AKIGKDKKEETYVLGKIKDIYSQNGFDTFIKKYNKDKTQFLMYQKDSLTWENVIEVIL
                               RDYPTTKKSEDGKNDVKCNPFEEYRRENGLICKYSKKGKGTPIKSLKYYDKKLGNCID
                               ITPEESRNKVILQSINPWRADVYFNPETLKYELMGLKYSDLSFEKGTGNYHISQEKYD
                               AIKEKEGIGKKSEFKFTLYRNDLILIKDIASGEQEIYRFLSRTMPNVNHYVELKPYDK
                               EKFDNVQELVEALGEADKVGRCIKGLNKPNISIYKVRTDVLGNKYFVKKKGDKPKLDF
                               KNNKK*"
               misc_feature    6659..6678
                               /label="SV40pro_F_primer"
                               /translation="MYPYDVPDYASPKKKRKVEASMTNGKILGLDIGIASVGVGIIEA
                               KTGKVVHANSRLFSAANAENNAERRGFRGSRRLNRRKKHRVKRVRDLFEKYGIVTDFR
                               NLNLNPYELRVKGLTEQLKNEELFAALRTISKRRGISYLDDAEDDSTGSTDYAKSIDE
                               NRRLLKNKTPGQIQLERLEKYGQLRGNFTVYDENGEAHRLINVFSTSDYEKEARKILE
                               TQADYNKKITAEFIDDYVEILTQKRKYYHGPGNEKSRTDYGRFRTDGTTLENIFGILI
                               GKCNFYPDEYRASKASYTAQEYNFLNDLNNLKVSTETGKLSTEQKESLVEFAKNTATL
                               GPAKLLKEIAKILDCKVDEIKGYREDDKGKPDLHTFEPYRKLKFNLESINIDDLSREV
                               IDKLADILTLNTEREGIEDAIKRNLPNQFTEEQISEIIKVRKSQSTAFNKGWHSFSAK
                               LMNELIPELYATSDEQMTILTRLEKFKVNKKSSKNTKTIDEKEVTDEIYNPVVAKSVR
                               QTIKIINAAVKKYGDFDKIVIEMPRDKNADDEKKFIDKRNKENKKEKDDALKRAAYLY
                               NSSDKLPDEVFHGNKQLETKIRLWYQQGERCLYSGKPISIQELVHNSNNFEIDHILPL
                               SLSFDDSLANKVLVYAWTNQEKGQKTPYQVIDSMDAAWSFREMKDYVLKQKGLGKKKR
                               DYLLTTENIDKIEVKKKFIERNLVDTRYASRVVLNSLQSALRELGKDTKVSVVRGQFT
                               SQLRRKWKIDKSRETYHHHAVDALIIAASSQLKLWEKQDNPMFVDYGKNQVVDKQTGE
                               ILSVSDDEYKELVFQPPYQGFVNTISSKGFEDEILFSYQVDSKYNRKVSDATIYSTRK
                               AKIGKDKKEETYVLGKIKDIYSQNGFDTFIKKYNKDKTQFLMYQKDSLTWENVIEVIL
                               RDYPTTKKSEDGKNDVKCNPFEEYRRENGLICKYSKKGKGTPIKSLKYYDKKLGNCID
                               ITPEESRNKVILQSINPWRADVYFNPETLKYELMGLKYSDLSFEKGTGNYHISQEKYD
                               AIKEKEGIGKKSEFKFTLYRNDLILIKDIASGEQEIYRFLSRTMPNVNHYVELKPYDK
                               EKFDNVQELVEALGEADKVGRCIKGLNKPNISIYKVRTDVLGNKYFVKKKGDKPKLDF
                               KNNKK*"
               CDS             6813..7607
                               /label="ORF frame 3"
                               /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                               PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                               LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                               EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                               QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
               gene            6816..7604
                               /label="NeoR/KanR"
                               /gene="NeoR/KanR"
                               /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                               PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                               LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                               EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                               QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
               CDS             complement(7122..7658)
                               /label="ORF frame 1"
                               /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                               VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                               VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                               PPHCISHDGYFLGRSKVR*"
               terminator      7784..7903
                               /label="SV40_PA_terminator"
                               /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                               VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                               VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                               PPHCISHDGYFLGRSKVR*"
               misc_feature    7872..7891
                               /label="EBV_rev_primer"
                               /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                               VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                               VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                               PPHCISHDGYFLGRSKVR*"
               terminator      8094..8129
                               /label="T7_terminator"
                               /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                               VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                               VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                               PPHCISHDGYFLGRSKVR*"
               misc_feature    complement(8152..8169)
                               /label="Baculovirs_rev_primer"
                               /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                               VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                               VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                               PPHCISHDGYFLGRSKVR*"
               misc_feature    8159..8236
                               /label="3_AcPH"
                               /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                               VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                               VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                               PPHCISHDGYFLGRSKVR*"
               CDS             complement(8266..8883)
                               /label="ORF frame 3"
                               /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                               NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                               QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                               KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
               misc_feature    8863..8896
                               /label="loxP"
                               /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                               NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                               QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                               KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
               gene            complement(9664..10524)
                               /label="Ampicillin"
                               /gene="Ampicillin"
                               /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                               NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                               QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                               KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
               CDS             complement(9664..10524)
                               /label="ORF frame 3"
                               /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                               IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                               YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                               DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                               LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                               EIGASLIKHW*"
          ORIGIN
              1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
             61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
            121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
            181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
            241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
            301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
            361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
            421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
            481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
            541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
            601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
            661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
            721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
            781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
            841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
            901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
            961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccc
           1021 tcgagtagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
           1081 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
           1141 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
           1201 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
           1261 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
           1321 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
           1381 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
           1441 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
           1501 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
           1561 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtgg atcttaatac
           1621 gactcactat aggggaattg tgagcggata acaattccgg gacctttaat tcaacccaac
           1681 acaatatatt atagttaaat aagaattatt atcaaatcat ttgtatatta attaaaatac
           1741 tatactgtaa attacatttt atttacaatc aacaagtttg tacaaaaaag caggctaagg
           1801 agatatacca ccggtgccac catgtaccca tacgatgttc cagattacgc ttcgccgaag
           1861 aaaaagcgca aggtcgaagc gtccatgact aacggcaaga ttctggggct ggacattggc
           1921 atcgcaagcg tgggggtggg gattattgag gcaaaaactg gaaaggtggt gcatgccaat
           1981 tcccggctgt tctctgccgc taacgctgag aacaatgcag aacggagagg gtttagggga
           2041 tctaggcgcc tgaatcgacg gaagaaacac cgcgtgaagc gagtccggga tctgttcgag
           2101 aaatacggaa tcgtcaccga ctttcgcaac ctgaatctga acccttatga gctgcgagtg
           2161 aagggcctga ccgaacagct gaaaaacgag gaactgttcg cagccctgag aacaatctct
           2221 aagagaaggg ggattagtta cctggacgat gccgaggacg atagtaccgg atcaacagac
           2281 tatgctaaga gcatcgatga gaatcgccga ctgctgaaaa acaagacacc aggccagatt
           2341 cagctggaga ggctggaaaa gtacggccag ctgcgcggga atttcaccgt ctatgacgag
           2401 aacggggaag cccatcgcct gatcaatgtg tttagtacat cagattacga gaaagaagca
           2461 cggaagatcc tggagacaca ggccgactac aacaagaaaa tcacagctga gttcattgac
           2521 gattatgtgg aaatcctgac ccagaaacga aagtactatc acggccccgg gaacgaaaag
           2581 agccggactg actacggacg gttccggacc gatgggacca cactggagaa tattttcgga
           2641 atcctgattg gcaagtgcaa cttttaccct gatgaatatc gagcaagcaa ggccagctac
           2701 accgcacagg agtataattt cctgaacgac ctgaacaatc tgaaggtgag caccgaaaca
           2761 gggaagctgt caacagagca gaaagaaagc ctggtggagt ttgccaagaa tactgctacc
           2821 ctgggacccg ctaaactgct gaaggagatc gcaaaaattc tggactgtaa ggtggatgag
           2881 atcaaaggat acagagagga cgataaaggc aagccagatc tgcatacctt cgagccctat
           2941 aggaaactga agtttaatct ggaaagcatc aacattgacg atctgtcccg cgaagtgatc
           3001 gacaagctgg ctgatattct gactctgaac accgagagag aaggaatcga ggacgcaatt
           3061 aagaggaatc tgccaaacca gttcacagag gaacagatca gcgagatcat caaggtgcgg
           3121 aagagccagt ccactgcttt caataagggc tggcactctt ttagtgcaaa actgatgaac
           3181 gagctgatcc ccgaactgta cgccacctcc gacgagcaga tgacaattct gactcggctg
           3241 gaaaaattca aggtcaataa gaaaagctcc aaaaacacaa agactatcga cgagaaggaa
           3301 gtcactgatg agatctacaa tcctgtggtc gccaagagcg tgagacagac catcaaaatc
           3361 attaacgctg cagtcaagaa atatggcgac ttcgataaga tcgtgattga aatgccacgg
           3421 gataaaaatg ctgacgatga gaagaagttc atcgacaaga gaaataagga gaacaagaag
           3481 gaaaaggacg atgccctgaa aagggccgct tacctgtata attctagtga caagctgccc
           3541 gatgaggtgt tccacggcaa caagcagctg gaaaccaaaa tccgactgtg gtatcagcag
           3601 ggggagcggt gcctgtatag tggaaagccc atctcaattc aggagctggt gcataactct
           3661 aacaatttcg aaatcgatca cattctgcct ctgtcactga gctttgacga tagtctggcc
           3721 aataaggtgc tggtctacgc ttggacaaac caggagaaag gccagaaaac cccttatcag
           3781 gtcatcgact ccatggatgc agcctggtct ttcagggaga tgaaggacta cgtgctgaaa
           3841 cagaagggac tgggcaagaa aaagcgcgac tatctgctga ctaccgagaa catcgataag
           3901 attgaagtga agaagaagtt catcgagagg aatctggtgg atactcgcta cgcatctcga
           3961 gtggtcctga actctctgca gagtgccctg agagagctgg ggaaagacac taaggtgtct
           4021 gtggtcaggg gacagttcac cagtcagctg cggagaaaat ggaagatcga taagagccgc
           4081 gagacatacc accatcacgc agtggacgcc ctgatcattg ctgcatcaag ccagctgaaa
           4141 ctgtgggaga agcaggacaa tcccatgttt gtggattatg gcaagaacca ggtggtcgac
           4201 aaacagactg gggagatcct gtccgtgtct gacgatgagt acaaggaact ggtgttccag
           4261 cccccttatc agggctttgt gaataccatc tcctctaaag ggttcgagga cgaaattctg
           4321 tttagctacc aggtggattc caaatataac cggaaggtca gtgacgcaac catctactca
           4381 acaagaaaag ccaagattgg caaggataag aaagaggaaa cctacgtgct gggaaaaatc
           4441 aaggacatct actcccagaa tggcttcgat accttcatca agaagtacaa caaagataag
           4501 actcagttcc tgatgtatca gaaggactct ctgacatggg agaacgtgat cgaagtcatt
           4561 ctgagggact acccaacaac taagaaaagc gaggacggca aaaatgatgt gaagtgcaac
           4621 ccctttgagg aatacaggcg cgagaatggg ctgatctgta agtattccaa gaaagggaaa
           4681 ggaactccca tcaagagcct gaagtactat gacaagaaac tggggaactg catcgatatt
           4741 accccagagg aatcacgcaa taaggtcatc ctgcagagca ttaacccttg gcgagccgac
           4801 gtgtacttca atccagagac actgaagtac gaactgatgg gcctgaaata ttcagatctg
           4861 agctttgaaa agggcactgg gaactaccat atcagccagg agaaatatga cgctatcaaa
           4921 gagaaggaag gaattggcaa gaaatccgag ttcaagttta cactgtaccg caacgacctg
           4981 atcctgatca aggatatcgc cagtggcgag caggaaatct acagattcct gtcaagaact
           5041 atgcccaatg tgaaccacta cgtcgagctg aagccttacg acaaggaaaa gttcgataac
           5101 gtgcaggagc tggtcgaagc actgggagag gcagataaag tgggacgatg tatcaaagga
           5161 ctgaataagc caaacatcag catctacaag gtgagaaccg acgtcctggg aaacaaatat
           5221 ttcgtgaaga aaaagggcga caaacccaag ctggatttta agaacaacaa gaagtaagaa
           5281 ttcggtaccg agctcaagct tgcggccgcc tatagtgtca cctaaatcgt aacccagctt
           5341 tcttgtacaa agtggtcggg tggcatccct gtgacccctc cccagtgcct ctcctggccc
           5401 tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt tgcatcattt
           5461 tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta tggagcaagg
           5521 ggcaagttgg gatgacaacc tgtagggcct gcggggtcta ttgggaacca agctggagtg
           5581 cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg attctcctgc
           5641 ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc taatttttgt
           5701 ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac tcctaatctc
           5761 aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga accactgctc
           5821 ccttccctgt ccttctagca taaccccttg gggcctctaa acgggtcttg aggggttttt
           5881 tgtctagaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
           5941 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
           6001 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
           6061 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
           6121 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
           6181 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
           6241 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
           6301 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
           6361 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
           6421 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
           6481 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
           6541 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
           6601 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
           6661 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
           6721 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
           6781 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
           6841 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
           6901 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
           6961 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
           7021 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
           7081 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
           7141 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
           7201 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
           7261 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
           7321 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
           7381 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
           7441 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
           7501 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
           7561 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
           7621 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
           7681 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
           7741 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
           7801 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
           7861 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gttcatgaag
           7921 ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt
           7981 tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat
           8041 cagaatgagt atttggttta gagtttggca acatatgccc atatgtaact agcataaccc
           8101 cttggggcct ctaaacgggt cttgaggggt tttttgctga aagcatgcgg aggaaattct
           8161 ccttgaagtt tccctggtgt tcaaagtaaa ggagtttgca ccagacgcac ctctgttcac
           8221 tggtccggcg tattaaaaca cgatacattg ttattagtac atttattaag cgctagattc
           8281 tgtgcgttgt tgatttacag acaattgttg tacgtatttt aataattcat taaatttata
           8341 atctttaggg tggtatgtta gagcgaaaat caaatgattt tcagcgtctt tatatctgaa
           8401 tttaaatatt aaatcctcaa tagatttgta aaataggttt cgattagttt caaacaaggg
           8461 ttgtttttcc gaaccgatgg ctggactatc taatggattt tcgctcaacg ccacaaaact
           8521 tgccaaatct tgtagcagca atctagcttt gtcgatattc gtttgtgttt tgttttgtaa
           8581 taaaggttcg acgtcgttca aaatattatg cgcttttgta tttctttcat cactgtcgtt
           8641 agtgtacaat tgactcgacg taaacacgtt aaatagagct tggacatatt taacatcggg
           8701 cgtgttagct ttattaggcc gattatcgtc gtcgtcccaa ccctcgtcgt tagaagttgc
           8761 ttccgaagac gattttgcca tagccacacg acgcctatta attgtgtcgg ctaacacgtc
           8821 cgcgatcaaa tttgtagttg agctttttgg aattgcgatc gcataacttc gtatagcata
           8881 cattatacga agttataagc tcggaacgct gcgctcggtc gttcggctgc ggcgagcggt
           8941 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
           9001 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
           9061 gtttttccat aggctccgcc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
           9121 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
           9181 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
           9241 agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
           9301 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
           9361 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
           9421 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
           9481 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
           9541 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
           9601 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
           9661 ttgttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
           9721 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
           9781 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
           9841 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
           9901 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
           9961 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
          10021 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
          10081 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
          10141 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
          10201 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
          10261 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
          10321 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
          10381 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
          10441 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
          10501 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
          10561 gggttattgt ctcatgtccg cgcgtt
          //
          
          

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