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          pMMB207

          價格:6500元

          聯系方式:I47-825O-882O

          買家導航

          載體基本信息

          出品公司: ATCC
          載體名稱: pMMB207
          質粒類型: 細菌表達載體;廣宿主載體
          高拷貝/低拷貝: 高拷貝
          克隆方法: 限制性內切酶,多克隆位點
          啟動子: Tac
          載體大小: 9075 bp
          5' 測序引物及序列 Tac promoter:GAGCGGATAACAATTTCACACAGG
          3' 測序引物及序列: pBAD Reverse: GATTTAATCTGTATCAGG
          載體標簽: --
          載體抗性: 氨芐青霉素和氯霉素(10 mg/ml Chloramphenicol)
          表達菌株: 原核細胞
          備注: --
          產品目錄號: 37809
          穩定性: 穩表達
          組成型/誘導型: 組成型
          病毒/非病毒: 非病毒

          載體質粒圖譜和多克隆位點信息

          pMMB207載體圖譜



          pMMB207 多克隆位點

          載體簡介

          載體序列

          LOCUS       pMMB207	9075 bp 	DNA	SYN
          DEFINITION  pMMB207
          ACCESSION   
          KEYWORDS    
          SOURCE      
            ORGANISM  other sequences; artificial sequences; vectors.
          FEATURES             Location/Qualifiers
               source          1..9075
                               /organism="pMMB207"
                               /mol_type="other DNA"
               misc_feature    complement(8..25)
                               /label="pBAD_rev_primer"
               misc_feature    complement(8..25)
                               /label="pTrcHis_rev_primer"
               terminator      58..215
                               /label="rrnB_terminator"
               terminator      181..224
                               /label="rrnB_T1_terminator"
               terminator      356..383
                               /label="rrnB_T2_terminator"
               promoter        424..452
                               /label="AmpR_promoter"
               gene            494..673
                               /label="Ampicillin (0 - 179)"
                               /gene="Ampicillin (0 - 179)"
               gene            1047..1706
                               /label="CAT/CamR"
                               /gene="CAT/CamR"
               CDS             1047..1706
                               /label="ORF frame 3"
               CDS             complement(2813..3469)
                               /label="ORF frame 3"
               CDS             2942..5071
                               /label="ORF frame 2"
               CDS             complement(4217..4810)
                               /label="ORF frame 3"
               CDS             4527..5171
                               /label="ORF frame 3"
               CDS             complement(5579..6346)
                               /label="ORF frame 3"
               CDS             5582..6421
                               /label="ORF frame 2"
               CDS             6309..7259
                               /label="ORF frame 3"
               promoter        7420..7450
                               /label="P5_promoter"
               misc_feature    complement(7536..8626)
                               /label="lacI"
               CDS             complement(7679..8494)
                               /label="ORF frame 3"
               promoter        8926..8954
                               /label="tac_promoter"
               misc_feature    8966..8988
                               /label="M13_pUC_rev_primer"
          ORIGIN
              1 ttttcagcct gatacagatt aaatcagaac gcagaagcgg tctgataaaa cagaatttgc
             61 ctggcggcag tagcgcggtg gtcccacctg accccatgcc gaactcagaa gtgaaacgcc
            121 gtagcgccga tggtagtgtg gggtctcccc atgcgagagt agggaactgc caggcatcaa
            181 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
            241 aacgctctcc tgagtaggac aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg
            301 cccggagggt ggcgggcagg acgcccgcca taaactgcca ggcatcaaat taagcagaag
            361 gccatcctga cggatggcct ttttgcgttt ctacaaactc ttttgtttat ttttctaaat
            421 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
            481 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
            541 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
            601 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
            661 gagttttcgc cccgaagaac gtgatgtccg gcggtgcttt tgccgttacg caccaccccg
            721 tcagtagctg aacaggaggg acagctgata gaaacagaag ccactggagc aagcacctca
            781 aaaacaccat catacactaa atcagtaagt tggcagcatc acccgacgca ctttgcgccg
            841 aataaatacc tgtgacggaa gatcacttcg cagaataaat aaatcctggt gtccctgttg
            901 ataccgggaa gccctgggcc aacttttggc gaaaatgaga cgttgatcgg cacgtaagag
            961 gttccaactt tcaccataat gaaataagat cactaccggg cgtatttttt gagttatcga
           1021 gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg
           1081 atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta
           1141 cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata
           1201 agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg
           1261 aattacgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt
           1321 acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg
           1381 atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg
           1441 cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga
           1501 gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca
           1561 ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc
           1621 atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact
           1681 gcgatgagtg gcagggcggg gcgtaatttt tttaaggcag ttattgtctt caaattcccg
           1741 ttgcacatag cccggcaatt cctttccctg ctctgccata agcgcagcga atgccgggta
           1801 atactcgtca acgatctgat agagaagggt ttgctcgggt cggtggctct ggtaacgacc
           1861 agtatcccga tcccggctgg ccgtcctggc cgccacatga ggcatgttcc gcgtccttgc
           1921 aatactgtgt ttacatacag tctatcgctt agcggaaagt tcttttaccc tcagccgaaa
           1981 tgcctgccgt tgctagacat tgccagccag tgcccgtcac tcccgtacta actgtcacga
           2041 acccctgcaa taactgtcac gcccccctgc aataactgtc acgaacccct gcaataactg
           2101 tcacgccccc aaacctgcaa acccagcagg ggcgggggct ggcggggtgt tggaaaaatc
           2161 catccatgat tatctaagaa taatccacta ggcgcggtta tcagcgccct tgtggggcgc
           2221 tgctgccctt gcccaatatg cccggccaga ggccggatag ctggtctatt cgctgcgcta
           2281 ggctacacac cgccccaccg ctgcgcggca gggggaaagg cgggcaaagc ccgctaaacc
           2341 ccacaccaaa ccccgcagaa atacgctgga gcgcttttag ccgctttagc ggcctttccc
           2401 cctacccgaa gggtgggggc gcgtgtgcag ccccgcaggg cctgtctcgg tcgatcattc
           2461 agcccggctc atccttctgg cgtggcggca gaccgaacaa ggcgcggtcg tggtcgcgtt
           2521 caaggtacgc atccattgcc gccatgagcc gatcctccgg ccactcgctg ctgttcacct
           2581 tggccaaaat catggccccc accagcacct tgcgccttgt ttcgttcttg cgctcttgct
           2641 gctgttccct tgcccgctcc cgctgaattt cggcattgat tcgcgctcgt tgttcttcga
           2701 gcttggccag ccgatccgcc gccttgttgc tccccttaac catcttgaca ccccattgtt
           2761 aatgtgctgt ctcgtaggct atcatggagg cacagcggcg gcaatcccga ccctactttg
           2821 taggggaggg cgcacttacc ggtttctctt cgagaaactg gcctaacggc cacccttcgg
           2881 gcggtgcgct ctccgagggc cattgcatgg agccgaaaag caaaagcaac agcgaggcag
           2941 catggcgatt tatcacctta cggcgaaaac cggcagcagg tcgggcggcc aatcggccag
           3001 ggccaaggcc gactacatcc agcgcgaagg caagtatgcc cgcgacatgg atgaagtctt
           3061 gcacgccgaa tccgggcaca tgccggagtt cgtcgagcgg cccgccgact actgggatgc
           3121 tgccgacctg tatgaacgcg ccaatgggcg gctgttcaag gaggtcgaat ttgccctgcc
           3181 ggtcgagctg accctcgacc agcagaaggc gctggcgtcc gagttcgccc agcacctgac
           3241 cggtgccgag cgcctgccgt atacgctggc catccatgcc ggtggcggcg agaacccgca
           3301 ctgccacctg atgatctccg agcggatcaa tgacggcatc gagcggcccg ccgctcagtg
           3361 gttcaagcgg tacaacggca agaccccgga gaagggcggg gcacagaaga ccgaagcgct
           3421 caagcccaag gcatggcttg agcagacccg cgaggcatgg gccgaccatg ccaaccgggc
           3481 attagagcgg gctggccacg acgcccgcat tgaccacaga acacttgagg cgcagggcat
           3541 cgagcgcctg cccggtgttc acctggggcc gaacgtggtg gagatggaag gccggggcat
           3601 ccgcaccgac cgggcagacg tggccctgaa catcgacacc gccaacgccc agatcatcga
           3661 cttacaggaa taccgggagg caatagacca tgaacgcaat cgacagagtg aagaaatcca
           3721 gaggcatcaa cgagttagcg gagcagatcg aaccgctggc ccagagcatg gcgacactgg
           3781 ccgacgaagc ccggcaggtc atgagccaga ccaagcaggc cagcgaggcg caggcggcgg
           3841 agtggctgaa agcccagcgc cagacagggg cggcatgggt ggagctggcc aaagagttgc
           3901 gggaggtagc cgccgaggtg agcagcgccg cgcagagcgc ccggagcgcg tcgcgggggt
           3961 ggcactggaa gctatggcta accgtgatgc tggcttccat gatgcctacg gtggtgctgc
           4021 tgatcgcatc gttgctcttg ctcgacctga cgccactgac aaccgaggac ggctcgatct
           4081 ggctgcgctt ggtggcccga tgaagaacga caggactttg caggccatag gccgacagct
           4141 caaggccatg ggctgtgagc gcttcgatat cggcgtcagg gacgcaccca ccggccagat
           4201 gatgaaccgg gaatggtcag ccgccgaagt gctccagaac acgccatggc tcaagcggat
           4261 gaatgcccag ggcaatgacg tgtatatcag gcccgccgag caggagcggc atggtctggt
           4321 gctggtggac gacctcagcg agtttgacct ggatgacatg aaagccgagg gccgggagcc
           4381 tgccctggta gtggaaacca gcccgaagaa ctatcaggca tgggtcaagg tggccgacgc
           4441 cgcaggcggt gaacttcggg ggcagattgc ccggacgctg gccagcgagt acgacgccga
           4501 cccggccagc gccgacagcc gccactatgg ccgcttggcg ggcttcacca accgcaagga
           4561 caagcacacc acccgcgccg gttatcagcc gtgggtgctg ctgcgtgaat ccaagggcaa
           4621 gaccgccacc gctggcccgg cgctggtgca gcaggctggc cagcagatcg agcaggccca
           4681 gcggcagcag gagaaggccc gcaggctggc cagcctcgaa ctgcccgagc ggcagcttag
           4741 ccgccaccgg cgcacggcgc tggacgagta ccgcagcgag atggccgggc tggtcaagcg
           4801 cttcggtcat gacctcagca agtgcgactt tatcgccgcg cagaagctgg ccagccgggg
           4861 ccgcagtgcc gaggaaatcg gcaaggccat ggccgaggcc agcccagcgc tggcagagcg
           4921 caagcccggc cacgaagcgg attacatcga gcgcaccgtc agcaaggtca tgggtctgcc
           4981 cagcgtccag cttgcgcggg ccgagctggc acgggcaccg gcaccccgcc agcgaggcat
           5041 ggacaggggc gggccagatt tcagcatgta gtgcttgcgt tggtactcac gcctgttata
           5101 ctatgagtac tcacgcacag aagggggttt tatggaatac gaaaaaagcg cttcagggtc
           5161 ggtctacctg atcaaaagtg acaagggcta ttggttgccc ggtggctttg gttatacgtc
           5221 aaacaaggcc gaggctggcc gcttttcagt cgctgatatg gccagcctta accttgacgg
           5281 ctgcaccttg tccttgttcc gcgaagacaa gcctttcggc cccggcaagt ttctcggtga
           5341 ctgatatgaa agaccaaaag gacaagcaga ccggcgacct gctggccagc cctgacgctg
           5401 tacgccaagc gcgatatgcc gagcgcatga aggccaaagg gatgcgtcag cgcaagttct
           5461 ggctgaccga cgacgaatac gaggcgctgc gcgagtgcct ggaagaactc agagcggcgc
           5521 agggcggggg tagtgacccc gccagcgcct aaccaccaac tgcctgcaaa ggaggcaatc
           5581 aatggctacc cataagccta tcaatattct ggaggcgttc gcagcagcgc cgccaccgct
           5641 ggactacgtt ttgcccaaca tggtggccgg tacggtcggg gcgctggtgt cgcccggtgg
           5701 tgccggtaaa tccatgctgg ccctgcaact ggccgcacag attgcaggcg ggccggatct
           5761 gctggaggtg ggcgaactgc ccaccggccc ggtgatctac ctgcccgccg aagacccgcc
           5821 caccgccatt catcaccgcc tgcacgccct tggggcgcac ctcagcgccg aggaacggca
           5881 agccgtggct gacggcctgc tgatccagcc gctgatcggc agcctgccca acatcatggc
           5941 cccggagtgg ttcgacggcc tcaagcgcgc cgccgagggc cgccgcctga tggtgctgga
           6001 cacgctgcgc cggttccaca tcgaggaaga aaacgccagc ggccccatgg cccaggtcat
           6061 cggtcgcatg gaggccatcg ccgccgatac cgggtgctct atcgtgttcc tgcaccatgc
           6121 cagcaagggc gcggccatga tgggcgcagg cgaccagcag caggccagcc ggggcagctc
           6181 ggtactggtc gataacatcc gctggcagtc ctacctgtcg agcatgacca gcgccgaggc
           6241 cgaggaatgg ggtgtggacg acgaccagcg ccggttcttc gtccgcttcg gtgtgagcaa
           6301 ggccaactat ggcgcaccgt tcgctgatcg gtggttcagg cggcatgacg gcggggtgct
           6361 caagcccgcc gtgctggaga ggcagcgcaa gagcaagggg gtgccccgtg gtgaagccta
           6421 agaacaagca cagcctcagc cacgtccggc acgacccggc gcactgtctg gcccccggcc
           6481 tgttccgtgc cctcaagcgg ggcgagcgca agcgcagcaa gctggacgtg acgtatgact
           6541 acggcgacgg caagcggatc gagttcagcg gcccggagcc gctgggcgct gatgatctgc
           6601 gcatcctgca agggctggtg gccatggctg ggcctaatgg cctagtgctt ggcccggaac
           6661 ccaagaccga aggcggacgg cagctccggc tgttcctgga acccaagtgg gaggccgtca
           6721 ccgctgaatg ccatgtggtc aaaggtagct atcgggcgct ggcaaaggaa atcggggcag
           6781 aggtcgatag tggtggggcg ctcaagcaca tacaggactg catcgagcgc ctttggaagg
           6841 tatccatcat cgcccagaat ggccgcaagc ggcaggggtt tcggctgctg tcggagtacg
           6901 ccagcgacga ggcggacggg cgcctgtacg tggccctgaa ccccttgatc gcgcaggccg
           6961 tcatgggtgg cggccagcat gtgcgcatca gcatggacga ggtgcgggcg ctggacagcg
           7021 aaaccgcccg cctgctgcac cagcggctgt gtggctggat cgaccccggc aaaaccggca
           7081 aggcttccat agataccttg tgcggctatg tctggccgtc agaggccagt ggttcgacca
           7141 tgcgcaagcg ccgcaagcgg gtgcgcgagg cgttgccgga gctggtcgcg ctgggctgga
           7201 cggtaaccga gttcgcggcg ggcaagtacg acatcacccg gcccaaggcg gcaggctgac
           7261 cccccccact ctattgtaaa caagacattt ttatctttta tattcaatgg cttattttcc
           7321 tgctaattgg taataccatg aaaaatacca tgctcagaaa aggcttaaca atattttgaa
           7381 aaattgccta ctgagcgctg ccgcacagct ccataggccg ctttcctggc tttgcttcca
           7441 gatgtatgct cttctgctcc cgaacgccag caagacgtag cccagcgcgt cggccagctt
           7501 gcaattcgcg ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
           7561 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
           7621 ttgggcgcca gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc
           7681 accgcctggc cctgagagag ttgcagcaag cggtccacgt ggtttgcccc agcaggcgaa
           7741 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt
           7801 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg
           7861 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca
           7921 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta
           7981 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg
           8041 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat
           8101 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct
           8161 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg
           8221 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat
           8281 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc
           8341 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca
           8401 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg
           8461 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt
           8521 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg
           8581 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct
           8641 cttccgggcg ctatcatgcc ataccgcgaa aggttttgca ccattcgatg gtgtcaacgt
           8701 aaatgccgct tcgccttcgc gcgcgaattg caagctgatc cgggcttatc gactgcacgg
           8761 tgcaccaatg cttctggcgt caggcagcca tcggaagctg tggtatggct gtgcaggtcg
           8821 taaatcactg cataattcgt gtcgctcaag gcgcactccc gttctggata atgttttttg
           8881 cgccgacatc ataacggttc tggcaaatat tctgaaatga gctgttgaca attaatcatc
           8941 ggctcgtata atgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagaattcg
           9001 agctcggtac ccggggatcc tctagagtcg acctgcaggc atgcaagctt ggctgttttg
           9061 gcggatgaga gaaga
          //
          

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