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          pCS22+

          價格:2500元

          聯系方式:I47-825O-882O

          買家導航

          載體基本信息

          出品公司: --
          載體名稱: pCS22+
          質粒類型: 克隆載體;哺乳動物表達載體;廣宿主載體;體外表達載體
          高拷貝/低拷貝: 高拷貝
          克隆方法: 限制性內切酶,多克隆位點
          啟動子: simian CMV IE94、lac、T3、SP6
          載體大小: 4046 bp
          5' 測序引物及序列: SP6 fwd(MCS1)、T3 fwd(MCS2)
          3' 測序引物及序列: EBV rev(MCS1)、SV40 polyA rev(MCS2)
          載體標簽:
          載體抗性: 氨芐青霉素
          克隆菌株: DH10B
          表達細胞: 原核細胞、哺乳動物、非洲爪蟾、鳥類、斑馬魚。
          備注: pCS22+載體能夠在多種細胞中進行高水平的瞬表達。pCS22+載體同時也能進行體外轉錄和翻譯。
          產品目錄號: --
          穩定性: 穩表達
          組成型/誘導型: 組成型
          病毒/非病毒: 非病毒

          載體質粒圖譜和多克隆位點信息

          pCS22(+)載體圖譜



          pCS22(+) 多克隆位點1

          pCS22(+) 多克隆位點2

          載體簡介

          載體序列

          LOCUS       Exported                4046 bp ds-DNA     circular SYN 31-JUL-2016
          DEFINITION  Vector that allows high-level transient expression in vertebrate 
                      cells as well as in vitro transcription/translation.
          KEYWORDS    pCS22+
          SOURCE      Biofeng Lab.
            ORGANISM  synthetic DNA construct
          REFERENCE   1  (bases 1 to 4046)
            AUTHORS   I.M.A.G.E. Consortium
          COMMENT     This file is created by Vector NTI
                      http://www.ningxiaxinwen.com/
          COMMENT     ORIGDB|GenBank
          COMMENT     VNTAUTHORNAME|biofeng.com|
          COMMENT     VNTNAME|pCS22+|
          FEATURES             Location/Qualifiers
               source          1..4046
                               /organism="synthetic DNA construct"
                               /mol_type="other DNA"
               promoter        34..52
                               /note="T7 promoter"
                               /note="promoter for bacteriophage T7 RNA polymerase"
               misc_feature    69..101
                               /note="MCS1"
               promoter        complement(103..121)
                               /note="T3 promoter"
                               /note="promoter for bacteriophage T3 RNA polymerase"
               polyA_signal    128..262
                               /note="SV40 poly(A) signal"
                               /note="SV40 polyadenylation signal"
               misc_feature    330..362
                               /note="MCS2"
               protein_bind    405..421
                               /bound_moiety="lac repressor encoded by lacI"
                               /note="lac operator"
                               /note="The lac repressor binds to the lac operator to 
                               inhibit transcription in E. coli. This inhibition can be 
                               relieved by adding lactose or 
                               isopropyl-beta-D-thiogalactopyranoside (IPTG)."
               promoter        complement(429..459)
                               /note="lac promoter"
                               /note="promoter for the E. coli lac operon"
               rep_origin      complement(783..1371)
                               /direction=LEFT
                               /note="ori"
                               /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                               replication"
               CDS             complement(1542..2402)
                               /codon_start=1
                               /gene="bla"
                               /product="beta-lactamase"
                               /note="AmpR"
                               /note="confers resistance to ampicillin, carbenicillin, and
                               related antibiotics"
                               /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                               ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                               PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                               EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                               LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                               LIKHW"
               promoter        complement(2403..2507)
                               /gene="bla"
                               /note="AmpR promoter"
               rep_origin      complement(2533..2988)
                               /direction=LEFT
                               /note="f1 ori"
                               /note="f1 bacteriophage origin of replication; arrow 
                               indicates direction of (+) strand synthesis"
               promoter        3062..4046
                               /note="CMV IE94 promoter"
                               /note="enhancer/promoter region of simian cytomegalovirus 
                               major immediate early transcription unit IE94"
          ORIGIN
                  1 cgccattctg cctggggacg tcggagcaag ctttaatacg actcactata gggcaataca
                 61 agctacttgt ttaaactctg cagggatccg aattcctcga gccctttagt gagggttaat
                121 ttacgtagat ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat
                181 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
                241 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
                301 gggggaggtg tgggaggttt tttaattcgc ggccgcggcg ccaatgcatt gggcccggta
                361 ccgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
                421 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
                481 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
                541 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
                601 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
                661 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
                721 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
                781 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
                841 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
                901 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
                961 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
               1021 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
               1081 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
               1141 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
               1201 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
               1261 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
               1321 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
               1381 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
               1441 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
               1501 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
               1561 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
               1621 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
               1681 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
               1741 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
               1801 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
               1861 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
               1921 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
               1981 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
               2041 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
               2101 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
               2161 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
               2221 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
               2281 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
               2341 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
               2401 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
               2461 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
               2521 aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
               2581 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
               2641 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
               2701 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
               2761 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
               2821 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
               2881 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
               2941 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat
               3001 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagt
               3061 cgaccatagc caattcaata tggcgtatat ggactcatgc caattcaata tggtggatct
               3121 ggacctgtgc caattcaata tggcgtatat ggactcgtgc caattcaata tggtggatct
               3181 ggaccccagc caattcaata tggcggactt ggcaccatgc caattcaata tggcggactt
               3241 ggcactgtgc caactgggga ggggtctact tggcacggtg ccaagtttga ggaggggtct
               3301 tggccctgtg ccaagtccgc catattgaat tggcatggtg ccaataatgg cggccatatt
               3361 ggctatatgc caggatcaat atataggcaa tatccaatat ggccctatgc caatatggct
               3421 attggccagg ttcaatacta tgtattggcc ctatgccata tagtattcca tatatgggtt
               3481 ttcctattga cgtagatagc ccctcccaat gggcggtccc atataccata tatggggctt
               3541 cctaataccg cccatagcca ctcccccatt gacgtcaatg gtctctatat atggtctttc
               3601 ctattgacgt catatgggcg gtcctattga cgtatatggc gcctccccca ttgacgtcaa
               3661 ttacggtaaa tggcccgcct ggctcaatgc ccattgacgt caataggacc acccaccatt
               3721 gacgtcaatg ggatggctca ttgcccattc atatccgttc tcacgccccc tattgacgtc
               3781 aatgacggta aatggcccac ttggcagtac atcaatatct attaatagta acttggcaag
               3841 tacattacta ttggaaggac gccagggtac attggcagta ctcccattga cgtcaatggc
               3901 ggtaaatggc ccgcgatggc tgccaagtac atccccattg acgtcaatgg ggaggggcaa
               3961 tgacgcaaat gggcgttcca ttgacgtaaa tgggcggtag gcgtgcctaa tgggaggtct
               4021 atataagcaa tgctcgttta gggaac
          //
          
          

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