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          pCS108

          價格:2500元

          聯系方式:I47-825O-882O

          買家導航

          載體基本信息

          出品公司: --
          載體名稱: pCS108
          質粒類型: 克隆載體;哺乳動物表達載體;廣宿主載體;體外表達載體
          高拷貝/低拷貝: 高拷貝
          克隆方法: 限制性內切酶,多克隆位點
          啟動子: lac、T3、SP6、CMV IE94
          載體大小: 4149 bp
          5' 測序引物及序列: SP6 fwd(MCS1)、T3 fwd(MCS2)
          3' 測序引物及序列: EBV rev(MCS1)、SV40 polyA rev(MCS2)
          載體標簽:
          載體抗性: 氨芐青霉素
          克隆菌株: DH10B
          表達細胞: 原核細胞、哺乳動物、非洲爪蟾、鳥類、斑馬魚。
          備注: pCS108載體能夠在多種細胞中進行高水平的瞬表達。pCS108載體同時也能進行體外轉錄和翻譯。
          產品目錄號: --
          穩定性: 瞬表達
          組成型/誘導型: --
          病毒/非病毒: 非病毒

          載體質粒圖譜和多克隆位點信息

          pCS108載體圖譜



          pCS108 多克隆位點1

          pCS108 多克隆位點2

          載體簡介

          載體序列

          LOCUS       Exported                4149 bp ds-DNA     circular SYN 29-JUL-2016
          DEFINITION  Vector that allows high-level transient expression in vertebrate 
                      cells as well as in vitro transcription/translation.
          ACCESSION   DQ649433
          VERSION     .
          KEYWORDS    pCS108
          SOURCE      synthetic DNA construct
            ORGANISM  synthetic DNA construct
          REFERENCE   1  (bases 1 to 4149)
            AUTHORS   .
            TITLE     Direct Submission
          FEATURES             Location/Qualifiers
               source          1..4149
                               /organism="synthetic DNA construct"
                               /mol_type="other DNA"
               promoter        35..53
                               /note="SP6 promoter"
                               /note="promoter for bacteriophage SP6 RNA polymerase"
               misc_feature    79..158
                               /note="MCS1"
               polyA_signal    190..324
                               /note="SV40 poly(A) signal"
                               /note="SV40 polyadenylation signal"
               misc_feature    393..428
                               /note="MCS2"
               promoter        complement(441..459)
                               /note="T3 promoter"
                               /note="promoter for bacteriophage T3 RNA polymerase"
               primer_bind     complement(480..496)
                               /note="M13 rev"
                               /note="common sequencing primer, one of multiple similar 
                               variants"
               misc_feature    complement(482..484)
                               /note="ATG"
               protein_bind    504..520
                               /bound_moiety="lac repressor encoded by lacI"
                               /note="lac operator"
                               /note="The lac repressor binds to the lac operator to 
                               inhibit transcription in E. coli. This inhibition can be 
                               relieved by adding lactose or 
                               isopropyl-beta-D-thiogalactopyranoside (IPTG)."
               promoter        complement(528..558)
                               /note="lac promoter"
                               /note="promoter for the E. coli lac operon"
               rep_origin      complement(882..1470)
                               /direction=LEFT
                               /note="ori"
                               /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                               replication"
               CDS             complement(1641..2501)
                               /codon_start=1
                               /gene="bla"
                               /product="beta-lactamase"
                               /note="AmpR"
                               /note="confers resistance to ampicillin, carbenicillin, and
                               related antibiotics"
                               /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                               ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                               PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                               EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                               LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                               LIKHW"
               promoter        complement(2502..2606)
                               /gene="bla"
                               /note="AmpR promoter"
               rep_origin      complement(2632..3087)
                               /direction=LEFT
                               /note="f1 ori"
                               /note="f1 bacteriophage origin of replication; arrow 
                               indicates direction of (+) strand synthesis"
               promoter        3165..4149
                               /note="CMV IE94 promoter"
                               /note="enhancer/promoter region of simian cytomegalovirus 
                               major immediate early transcription unit IE94"
          ORIGIN
                  1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
                 61 ctacttgttc tttttgcagg atcccatcga ttcgaattcg tcgacaggcc aatctggccg
                121 cggccgcaag gcctgatatc tctagaccgc ggctcgagcc tctcgcccta tagtgagtcg
                181 tattacgtag atccagacat gataagatac attgatgagt ttggacaaac cacaactaga
                241 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
                301 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
                361 cagggggagg tgtgggaggt tttttaattc gcggcgcgcc gcggcgccaa tgcattgggc
                421 ccggtaccca gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg
                481 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
                541 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
                601 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
                661 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
                721 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
                781 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
                841 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
                901 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
                961 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
               1021 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
               1081 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
               1141 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
               1201 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
               1261 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
               1321 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
               1381 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
               1441 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
               1501 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
               1561 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
               1621 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
               1681 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
               1741 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
               1801 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
               1861 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
               1921 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
               1981 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
               2041 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
               2101 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
               2161 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
               2221 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
               2281 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
               2341 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
               2401 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
               2461 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
               2521 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
               2581 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa
               2641 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
               2701 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
               2761 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
               2821 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
               2881 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
               2941 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
               3001 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
               3061 cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc aactgttggg
               3121 aagggcgatc ggtgcgggcc tcttcgctat tacgccagtc gatcgaccat agccaattca
               3181 atatggcgta tatggactca tgccaattca atatggtgga tctggacctg tgccaattca
               3241 atatggcgta tatggactcg tgccaattca atatggtgga tctggacccc agccaattca
               3301 atatggcgga cttggcacca tgccaattca atatggcgga cttggcactg tgccaactgg
               3361 ggaggggtct acttggcacg gtgccaagtt tgaggagggg tcttggccct gtgccaagtc
               3421 cgccatattg aattggcatg gtgccaataa tggcggccat attggctata tgccaggatc
               3481 aatatatagg caatatccaa tatggcccta tgccaatatg gctattggcc aggttcaata
               3541 ctatgtattg gccctatgcc atatagtatt ccatatatgg gttttcctat tgacgtagat
               3601 agcccctccc aatgggcggt cccatatacc atatatgggg cttcctaata ccgcccatag
               3661 ccactccccc attgacgtca atggtctcta tatatggtct ttcctattga cgtcatatgg
               3721 gcggtcctat tgacgtatat ggcgcctccc ccattgacgt caattacggt aaatggcccg
               3781 cctggctcaa tgcccattga cgtcaatagg accacccacc attgacgtca atgggatggc
               3841 tcattgccca ttcatatccg ttctcacgcc ccctattgac gtcaatgacg gtaaatggcc
               3901 cacttggcag tacatcaata tctattaata gtaacttggc aagtacatta ctattggaag
               3961 tacgccaggg tacattggca gtactcccat tgacgtcaat ggcggtaaat ggcccgcgat
               4021 ggctgccaag tacatcccca ttgacgtcaa tggggagggg caatgacgca aatgggcgtt
               4081 ccattgacgt aaatgggcgg taggcgtgcc taatgggagg tctatataag caatgctcgt
               4141 ttagggaac
          //
          
          
          
          

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