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      1. <pre id="q7rs8"></pre>
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          pBTL-1

          價格:2500元

          聯系方式:I47-825O-882O

          買家導航

          載體基本信息

          出品人: Lynch,M.D. and Gill,R.T.
          載體名稱: pBTL-1
          質粒類型: 廣宿主載體;克隆載體;原核表達載體
          高拷貝/低拷貝: --
          克隆方法: 限制性內切酶,多克隆位點
          啟動子: lac
          載體大小: 2662 bp
          5' 測序引物及序列: --
          3' 測序引物及序列: --
          載體標簽: --
          載體抗性: 氨芐青霉素
          克隆菌株: DH5α 等
          宿主細胞(系): 多個菌屬
          備注: --
          產品目錄號: --
          組成型/誘導型: 誘導型

          載體質粒圖譜和多克隆位點信息

          pBTL-1載體圖譜

          載體簡介

          載體序列

          LOCUS       DQ058739                2662 bp    DNA     circular SYN 08-MAY-2006
          DEFINITION  Broad host range vector pBTL-1, complete sequence.
          ACCESSION   DQ058739
          VERSION     DQ058739.1  GI:69048446
            AUTHORS   Lynch,M.D. and Gill,R.T.
            TITLE     Broad host range vectors for stable genomic library construction
             PUBMED   16496398
            JOURNAL   Submitted (12-MAY-2005) Chemical & Biological Engineering,
                      University of Colorado, ECCH 111, Campus Box 424, Boulder, CO
                      80309, USA
          FEATURES             Location/Qualifiers
               source          1..2662
                               /organism="Broad host range vector pBTL-1"
                               /mol_type="other DNA"
                               /db_xref="taxon:334971"
               regulatory      60..96
                               /regulatory_class="terminator"
                               /note="SoxR terminator"
               gene            872..1534
                               /gene="rep"
                               /note="rep gene from pBBR1, GenBank Accession Number
                               X66730"
               CDS             872..1534
                               /gene="rep"
                               /note="required for replication"
                               /codon_start=1
                               /transl_table=11
                               /product="Rep"
                               /protein_id="AAY99747.1"
                               /db_xref="GI:69048447"
                               /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLL
                               HHLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAY
                               VVNDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGP
                               GEEPPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLP
                               RR"
               gene            1631..2491
                               /gene="bla"
                               /note="beta-lactam resistance"
               CDS             1631..2491
                               /gene="bla"
                               /codon_start=1
                               /transl_table=11
                               /product="beta-lactamase"
                               /protein_id="AAY99748.1"
                               /db_xref="GI:69048448"
                               /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                               IELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                               YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRL
                               DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSL
                               LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                               EIGASLIKHW"
               regulatory      2507..2538
                               /regulatory_class="terminator"
                               /note="TonB terminator"
               regulatory      2565..2647
                               /regulatory_class="promoter"
                               /note="lac promoter"
          ORIGIN      
                  1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga
                 61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgcggccacc ggctggctcg
                121 cttcgctcgg cccgtggaca accctgctgg acaagctgat ggacaggctg cgcctgccca
                181 cgagcttgac cacagggatt gcccaccggc tacccagcct tcgaccacat acccaccggc
                241 tccaactgcg cggcctgcgg ccttgcccca tcaatttttt taattttctc tggggaaaag
                301 cctccggcct gcggcctgcg cgcttcgctt gccggttgga caccaagtgg aaggcgggtc
                361 aaggctcgcg cagcgaccgc gcagcggctt ggccttgacg cgcctggaac gacccaagcc
                421 tatgcgagtg ggggcagtcg aaggcgaagc ccgcccgcct gccccccgag cctcacggcg
                481 gcgagtgcgg gggttccaag ggggcagcgc caccttgggc aaggccgaag gccgcgcagt
                541 cgatcaacaa gccccggagg ggccactttt tgccggaggg ggagccgcgc cgaaggcgtg
                601 ggggaacccc gcaggggtgc ccttctttgg gcaccaaaga actagatata gggcgaaatg
                661 cgaaagactt aaaaatcaac aacttaaaaa aggggggtac gcaacagctc attgcggcac
                721 cccccgcaat agctcattgc gtaggttaaa gaaaatctgt aattgactgc cacttttacg
                781 caacgcataa ttgttgtcgc gctgccgaaa agttgcagct gattgcgcat ggtgccgcaa
                841 ccgtgcggca ccctaccgca tggagataag catggccacg cagtccagag aaatcggcat
                901 tcaagccaag aacaagcccg gtcactgggt gcaaacggaa cgcaaagcgc atgaggcgtg
                961 ggccgggctt attgcgagga aacccacggc ggcaatgctg ctgcatcacc tcgtggcgca
               1021 gatgggccac cagaacgccg tggtggtcag ccagaagaca ctttccaagc tcatcggacg
               1081 ttctttgcgg acggtccaat acgcagtcaa ggacttggtg gccgagcgct ggatctccgt
               1141 cgtgaagctc aacggccccg gcaccgtgtc ggcctacgtg gtcaatgacc gcgtggcgtg
               1201 gggccagccc cgcgaccagt tgcgcctgtc ggtgttcagt gccgccgtgg tggttgatca
               1261 cgacgaccag gacgaatcgc tgttggggca tggcgacctg cgccgcatcc cgaccctgta
               1321 tccgggcgag cagcaactac cgaccggccc cggcgaggag ccgcccagcc agcccggcat
               1381 tccgggcatg gaaccagacc tgccagcctt gaccgaaacg gaggaatggg aacggcgcgg
               1441 gcagcagcgc ctgccgatgc ccgatgagcc gtgttttctg gacgatggcg agccgttgga
               1501 gccgccgaca cgggtcacgc tgccgcgccg gtagtacgta agaggttcca actttcacca
               1561 taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa
               1621 ggaagctaaa atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
               1681 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
               1741 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
               1801 tttacgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
               1861 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
               1921 gaatgacttg gttgagtact caccagtcac agaaaagcat ctcacggatg gcatgacagt
               1981 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
               2041 ggcaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
               2101 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
               2161 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
               2221 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggatc
               2281 acttctgcgc tcggccctcc cggctggctg gtttattgct gataaatctg gagccggtga
               2341 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgcatcgt
               2401 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
               2461 gataggtgcc tcactgatta agcattggta atgaggatcc ccctcaagtc aaaagcctcc
               2521 ggtcggaggc ttttgacttt ctgctatgga ggtcaggtat gattttgcat taggcacccc
               2581 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
               2641 ttcacactct agagaattcg tc
          //
          

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