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          pBMTB-4

          價格:2500元

          聯系方式:I47-825O-882O

          買家導航

          載體基本信息

          出品人: Lynch,M.D. and Gill,R.T.
          載體名稱: pBMTB-4
          質粒類型: 廣宿主載體;克隆載體;原核表達載體
          高拷貝/低拷貝: --
          克隆方法: 限制性內切酶,多克隆位點
          啟動子: araBAD
          載體大小: 5363 bp
          5' 測序引物及序列: --
          3' 測序引物及序列: --
          載體標簽: --
          載體抗性: 四環素
          克隆菌株: DH5α 等
          宿主細胞(系): 多個菌屬
          備注: --
          產品目錄號: --
          組成型/誘導型: 誘導型

          載體質粒圖譜和多克隆位點信息

          pBMTB-4載體圖譜



          載體簡介

          載體序列

          LOCUS       DQ058727                5363 bp    DNA     circular SYN 08-MAY-2006
          DEFINITION  Broad host range vector pBMTB-4, complete sequence.
          ACCESSION   DQ058727
            AUTHORS   Lynch,M.D. and Gill,R.T.
            TITLE     Broad host range vectors for stable genomic library construction
            JOURNAL   Submitted (12-MAY-2005) Chemical & Biological Engineering,
                      University of Colorado, ECCH 111, Campus Box 424, Boulder, CO
                      80309, USA
          FEATURES             Location/Qualifiers
               source          1..5363
                               /organism="Broad host range vector pBMTB-4"
                               /mol_type="other DNA"
                               /db_xref="taxon:334959"
               regulatory      60..96
                               /regulatory_class="terminator"
                               /note="SoxR terminator"
               gene            complement(104..1123)
                               /gene="mob"
                               /note="mob gene from pBBR1MCS, GenBank Accession Number
                               U02374"
               CDS             complement(104..1123)
                               /gene="mob"
                               /note="required for mobilization"
                               /codon_start=1
                               /transl_table=11
                               /product="Mob"
                               /protein_id="AAY99709.1"
                               /db_xref="GI:69048280"
                               /translation="MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEH
                               WAASSTDEAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWKSASQEQQAAFFEKAHKW
                               LADKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLSAKEFIGNKAQMTRDQTTFAA
                               AVADLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAPQGLAEKLG
                               ISKRVETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVL
                               DALGPLNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPEKCHLTSK
                               KPLLS"
               gene            2117..2779
                               /gene="rep"
                               /note="rep gene from pBBR1, GenBank Accession Number
                               X66730"
               CDS             2117..2779
                               /gene="rep"
                               /note="required for replication"
                               /codon_start=1
                               /transl_table=11
                               /product="Rep"
                               /protein_id="AAY99710.1"
                               /db_xref="GI:69048281"
                               /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLL
                               HHLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAY
                               VVNDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGP
                               GEEPPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLP
                               RR"
               gene            2876..4066
                               /gene="tetA"
                               /note="tetracycline resistance gene from pBR322"
               CDS             2876..4066
                               /gene="tetA"
                               /codon_start=1
                               /transl_table=11
                               /product="tetracycline/H+ antiporter"
                               /protein_id="AAY99711.1"
                               /db_xref="GI:69048282"
                               /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH
                               YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG
                               RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH
                               APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV
                               FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE
                               KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG
                               QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR
                               ATST"
               regulatory      4086..4117
                               /regulatory_class="terminator"
                               /note="TonB terminator"
               gene            complement(4145..5074)
                               /gene="araC"
               CDS             complement(4145..5074)
                               /gene="araC"
                               /note="regulator"
                               /codon_start=1
                               /transl_table=11
                               /product="AraC"
                               /protein_id="AAY99712.1"
                               /db_xref="GI:69048283"
                               /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE
                               ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH
                               PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG
                               EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA
                               QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL
                               YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS"
               regulatory      5082..5349
                               /regulatory_class="promoter"
                               /note="pBAD promoter"
          ORIGIN      
                  1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga
                 61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgctcatgat aataatggtt
                121 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg cccgcgttcc tgctggcgct
                181 gggcctgttt ctggcgctgg acttcccgct gttccgtcag cagcttttcg cccacggcct
                241 tgatgatcgc ggcggccttg gcctgcatat cccgattcaa cggccccagg gcgtccagaa
                301 cgggcttcag gcgctcccga aggtctcggg ccgtctcttg ggcttgatcg gccttcttgc
                361 gcatctcacg cgctcctgcg gcggcctgta gggcaggctc atacccctgc cgaaccgctt
                421 ttgtcagccg gtcggccacg gcttccggcg tctcaacgcg ctttgagatt cccagctttt
                481 cggccaatcc ctgcggtgca taggcgcgtg gctcgaccgc ttgcgggctg atggtgacgt
                541 ggcccactgg tggccgctcc agggcctcgt agaacgcctg aatgcgcgtg tgacgtgcct
                601 tgctgccctc gatgccccgt tgcagcccta gatcggccac agcggccgca aacgtggtct
                661 ggtcgcgggt catctgcgct ttgttgccga tgaactcctt ggccgacagc ctgccgtcct
                721 gcgtcagcgg caccacgaac gcggtcatgt gcgggctggt ttcgtcacgg tggatgctgg
                781 ccgtcacgat gcgatccgcc ccgtacttgt ccgccagcca cttgtgcgcc ttctcgaaga
                841 acgccgcctg ctgttcttgg ctggccgact tccaccattc cgggctggcc gtcatgacgt
                901 actcgaccgc caacacagcg tccttgcgcc gcttctctgg cagcaactcg cgcagtcggc
                961 ccatcgcttc atcggtgctg ctggccgccc agtgctcgtt ctctggcgtc ctgctggcgt
               1021 cagcgttggg cgtctcgcgc tcgcggtagg cgtgcttgag actggccgcc acgttgccca
               1081 ttttcgccag cttcttgcat cgcatgatcg cgtatgccgc catgcctgcc cctccctttt
               1141 ggtgtccaac cggctcgacg ggggcagcgc aaggcggtgc ctccggcggg ccactcaatg
               1201 cttgagtata ctcactagac tttgcttcgc aaagtcgtga ccgcctacgg cggctgcggc
               1261 gccctacggg cttgctctcc gggcttcgcc ctgcgcggtc gctgcgctcc cttgccagcc
               1321 cgtggatatg tggacgatgg ccgcgagcgg ccaccggctg gctcgcttcg ctcggcccgt
               1381 ggacaaccct gctggacaag ctgatggaca ggctgcgcct gcccacgagc ttgaccacag
               1441 ggattgccca ccggctaccc agccttcgac cacataccca ccggctccaa ctgcgcggcc
               1501 tgcggccttg ccccatcaat ttttttaatt ttctctgggg aaaagcctcc ggcctgcggc
               1561 ctgcgcgctt cgcttgccgg ttggacacca agtggaaggc gggtcaaggc tcgcgcagcg
               1621 accgcgcagc ggcttggcct tgacgcgcct ggaacgaccc aagcctatgc gagtgggggc
               1681 agtcgaaggc gaagcccgcc cgcctgcccc ccgagcctca cggcggcgag tgcgggggtt
               1741 ccaagggggc agcgccacct tgggcaaggc cgaaggccgc gcagtcgatc aacaagcccc
               1801 ggaggggcca ctttttgccg gagggggagc cgcgccgaag gcgtggggga accccgcagg
               1861 ggtgcccttc tttgggcacc aaagaactag atatagggcg aaatgcgaaa gacttaaaaa
               1921 tcaacaactt aaaaaagggg ggtacgcaac agctcattgc ggcacccccc gcaatagctc
               1981 attgcgtagg ttaaagaaaa tctgtaattg actgccactt ttacgcaacg cataattgtt
               2041 gtcgcgctgc cgaaaagttg cagctgattg cgcatggtgc cgcaaccgtg cggcacccta
               2101 ccgcatggag ataagcatgg ccacgcagtc cagagaaatc ggcattcaag ccaagaacaa
               2161 gcccggtcac tgggtgcaaa cggaacgcaa agcgcatgag gcgtgggccg ggcttattgc
               2221 gaggaaaccc acggcggcaa tgctgctgca tcacctcgtg gcgcagatgg gccaccagaa
               2281 cgccgtggtg gtcagccaga agacactttc caagctcatc ggacgttctt tgcggacggt
               2341 ccaatacgca gtcaaggact tggtggccga gcgctggatc tccgtcgtga agctcaacgg
               2401 ccccggcacc gtgtcggcct acgtggtcaa tgaccgcgtg gcgtggggcc agccccgcga
               2461 ccagttgcgc ctgtcggtgt tcagtgccgc cgtggtggtt gatcacgacg accaggacga
               2521 atcgctgttg gggcatggcg acctgcgccg catcccgacc ctgtatccgg gcgagcagca
               2581 actaccgacc ggccccggcg aggagccgcc cagccagccc ggcattccgg gcatggaacc
               2641 agacctgcca gccttgaccg aaacggagga atgggaacgg cgcgggcagc agcgcctgcc
               2701 gatgcccgat gagccgtgtt ttctggacga tggcgagccg ttggagccgc cgacacgggt
               2761 cacgctgccg cgccggtagt acgtaagagg ttccaacttt caccataatg aaataagatc
               2821 actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgaa
               2881 atctaacaat gcgctcatcg tcatcctcgg caccgtcacc ctggatgctg taggcatagg
               2941 cttggttatg ccggtactgc cgggcctctt gcgggatatc gtccattccg acagcatcgc
               3001 cagtcactat ggcgtgctgc tagcgctata tgcgttgatg caatttctat gcgcacccgt
               3061 tctcggagca ctgtccgacc gctttggccg ccgcccagtc ctgctcgctt cgctacttgg
               3121 agccactatc gactacgcga tcatggcgac cacacccgtc ctgtggatcc tctacgccgg
               3181 acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga
               3241 catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt
               3301 gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc
               3361 attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca
               3421 ggagtcgcat aagggagagc gtcgaccgat gcccttgaga gccttcaacc cagtcagctc
               3481 cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat
               3541 gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg accgctttcg
               3601 ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc
               3661 tcaagccttc gtcactggtc ccgccaccaa acgtttcggc gagaagcagg ccattatcgc
               3721 cggcatggcg gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc gaggctggat
               3781 ggccttcccc attatgattc ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc
               3841 catgctgtcc aggcaggtag atgacgacca tcagggacag cttcaaggat cgctcgcggc
               3901 tcttaccagc ctaacttcga tcactggacc gctgatcgtc acggcgattt atgccgcctc
               3961 ggcgagcaca tggaacgggt tggcatggat tgtaggcgcc gccctatacc ttgtctgcct
               4021 ccccgcgttg cgtcgcggtg catggagccg ggccacctcg acctgaatgg aagggatccc
               4081 cctcaagtca aaagcctccg gtcggaggct tttgactttc tgctatggag gtcaggtatg
               4141 atttttatga caacttgacg gctacatcat tcactttttc ttcacaaccg gcacggaact
               4201 cgctcgggct ggccccggtg cattttttaa atacccgcga gaaatagagt tgatcgtcaa
               4261 aaccaacatt gcgaccgacg gtggcgatag gcatccgggt ggtgctcaaa agcagcttcg
               4321 cctggctgat acgttggtcc tcgcgccagc ttaagacgct aatccctaac tgctggcgga
               4381 aaagatgtga cagacgcgac ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa
               4441 aattgctgtc tgccaggtga tcgctgatgt actgacaagc ctcgcgtacc cgattatcca
               4501 tcggtggatg gagcgactcg ttaatcgctt ccatgcgccg cagtaacaat tgctcaagca
               4561 gatttatcgc cagcagctcc gaatagcgcc cttccccttg cccggcgtta atgatttgcc
               4621 caaacaggtc gctgaaatgc ggctggtgcg cttcatccgg gcgaaagaac cccgtattgg
               4681 caaatattga cggccagtta agccattcat gccagtaggc gcgcggacga aagtaaaccc
               4741 actggtgata ccattcgcga gcctccggat gacgaccgta gtgatgaatc tctcctggcg
               4801 ggaacagcaa aatatcaccc ggtcggcaaa caaattctcg tccctgattt ttcaccaccc
               4861 cctgaccgcg aatggtgaga ttgagaatat aacctttcat tcccagcggt cggtcgataa
               4921 aaaaatcgag ataaccgttg gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa
               4981 acgagtatcc cggcagcagg ggatcatttt gcgcttcagc catacttttc atactcccgc
               5041 cattcagaga agaaaccaat tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt
               5101 actggctctt ctcgctaacc aaaccggtaa ccccgcttat taaaagcatt ctgtaacaaa
               5161 gcgggaccaa agccatgaca aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag
               5221 tccacattga ttatttgcac ggcgtcacac tttgctatgc catagcattt ttatccataa
               5281 gattagcgga tcctacctga cgctttttat cgcaactctc tactgtttct ccatacccgt
               5341 ttttttgggc tagagaattc gtc
          //
          

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