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      1. <pre id="q7rs8"></pre>
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          pBMT-2

          價格:3000元

          聯系方式:I47-825O-882O

          買家導航

          載體基本信息

          出品人: Lynch,M.D. and Gill,R.T.
          載體名稱: pBMT-2
          質粒類型: 廣宿主載體;克隆載體;原核載體
          高拷貝/低拷貝: --
          克隆方法: 限制性內切酶,多克隆位點
          啟動子:
          載體大小: 3803 bp
          5' 測序引物及序列: --
          3' 測序引物及序列: --
          載體標簽: --
          載體抗性: 卡那霉素
          克隆菌株: DH5α 等
          宿主細胞(系): 多個菌屬
          備注: --
          產品目錄號: --
          組成型/誘導型: 組成型

          載體質粒圖譜和多克隆位點信息

          pBMT-2載體圖譜



          載體簡介

          載體序列

          LOCUS       DQ058715                3803 bp    DNA     circular SYN 08-MAY-2006
          DEFINITION  Broad host range vector pBMT-2, complete sequence.
          ACCESSION   DQ058715
          VERSION     DQ058715.1  GI:69048029
            AUTHORS   Lynch,M.D. and Gill,R.T.
            TITLE     Direct Submission
            JOURNAL   Submitted (12-MAY-2005) Chemical & Biological Engineering,
                      University of Colorado, ECCH 111, Campus Box 424, Boulder, CO
                      80309, USA
          FEATURES             Location/Qualifiers
               source          1..3803
                               /organism="Broad host range vector pBMT-2"
                               /mol_type="other DNA"
                               /db_xref="taxon:334947"
               regulatory      60..96
                               /regulatory_class="terminator"
                               /note="SoxR terminator"
               gene            complement(104..1123)
                               /gene="mob"
                               /note="mob gene from pBBR1MCS, GenBank Accession Number
                               U02374"
               CDS             complement(104..1123)
                               /gene="mob"
                               /note="required for mobilization"
                               /codon_start=1
                               /transl_table=11
                               /product="Mob"
                               /protein_id="AAY99675.1"
                               /db_xref="GI:69048030"
                               /translation="MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEH
                               WAASSTDEAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWKSASQEQQAAFFEKAHKW
                               LADKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLSAKEFIGNKAQMTRDQTTFAA
                               AVADLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAPQGLAEKLG
                               ISKRVETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVL
                               DALGPLNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPEKCHLTSK
                               KPLLS"
               gene            2117..2779
                               /gene="rep"
                               /note="rep gene from pBBR1, GenBank Accession Number
                               X66730"
               CDS             2117..2779
                               /gene="rep"
                               /note="required for replication"
                               /codon_start=1
                               /transl_table=11
                               /product="Rep"
                               /protein_id="AAY99676.1"
                               /db_xref="GI:69048031"
                               /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLL
                               HHLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAY
                               VVNDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGP
                               GEEPPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLP
                               RR"
               gene            2876..3691
                               /gene="kan"
                               /note="kanamycin resistance gene from TN903"
               CDS             2876..3691
                               /gene="kan"
                               /codon_start=1
                               /transl_table=11
                               /product="kanamycin kinase"
                               /protein_id="AAY99677.1"
                               /db_xref="GI:69048032"
                               /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                               GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                               GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGL
                               VGASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                               RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
               regulatory      3708..3739
                               /regulatory_class="terminator"
                               /note="TonB terminator"
          ORIGIN      
                  1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga
                 61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgctcatgat aataatggtt
                121 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg cccgcgttcc tgctggcgct
                181 gggcctgttt ctggcgctgg acttcccgct gttccgtcag cagcttttcg cccacggcct
                241 tgatgatcgc ggcggccttg gcctgcatat cccgattcaa cggccccagg gcgtccagaa
                301 cgggcttcag gcgctcccga aggtctcggg ccgtctcttg ggcttgatcg gccttcttgc
                361 gcatctcacg cgctcctgcg gcggcctgta gggcaggctc atacccctgc cgaaccgctt
                421 ttgtcagccg gtcggccacg gcttccggcg tctcaacgcg ctttgagatt cccagctttt
                481 cggccaatcc ctgcggtgca taggcgcgtg gctcgaccgc ttgcgggctg atggtgacgt
                541 ggcccactgg tggccgctcc agggcctcgt agaacgcctg aatgcgcgtg tgacgtgcct
                601 tgctgccctc gatgccccgt tgcagcccta gatcggccac agcggccgca aacgtggtct
                661 ggtcgcgggt catctgcgct ttgttgccga tgaactcctt ggccgacagc ctgccgtcct
                721 gcgtcagcgg caccacgaac gcggtcatgt gcgggctggt ttcgtcacgg tggatgctgg
                781 ccgtcacgat gcgatccgcc ccgtacttgt ccgccagcca cttgtgcgcc ttctcgaaga
                841 acgccgcctg ctgttcttgg ctggccgact tccaccattc cgggctggcc gtcatgacgt
                901 actcgaccgc caacacagcg tccttgcgcc gcttctctgg cagcaactcg cgcagtcggc
                961 ccatcgcttc atcggtgctg ctggccgccc agtgctcgtt ctctggcgtc ctgctggcgt
               1021 cagcgttggg cgtctcgcgc tcgcggtagg cgtgcttgag actggccgcc acgttgccca
               1081 ttttcgccag cttcttgcat cgcatgatcg cgtatgccgc catgcctgcc cctccctttt
               1141 ggtgtccaac cggctcgacg ggggcagcgc aaggcggtgc ctccggcggg ccactcaatg
               1201 cttgagtata ctcactagac tttgcttcgc aaagtcgtga ccgcctacgg cggctgcggc
               1261 gccctacggg cttgctctcc gggcttcgcc ctgcgcggtc gctgcgctcc cttgccagcc
               1321 cgtggatatg tggacgatgg ccgcgagcgg ccaccggctg gctcgcttcg ctcggcccgt
               1381 ggacaaccct gctggacaag ctgatggaca ggctgcgcct gcccacgagc ttgaccacag
               1441 ggattgccca ccggctaccc agccttcgac cacataccca ccggctccaa ctgcgcggcc
               1501 tgcggccttg ccccatcaat ttttttaatt ttctctgggg aaaagcctcc ggcctgcggc
               1561 ctgcgcgctt cgcttgccgg ttggacacca agtggaaggc gggtcaaggc tcgcgcagcg
               1621 accgcgcagc ggcttggcct tgacgcgcct ggaacgaccc aagcctatgc gagtgggggc
               1681 agtcgaaggc gaagcccgcc cgcctgcccc ccgagcctca cggcggcgag tgcgggggtt
               1741 ccaagggggc agcgccacct tgggcaaggc cgaaggccgc gcagtcgatc aacaagcccc
               1801 ggaggggcca ctttttgccg gagggggagc cgcgccgaag gcgtggggga accccgcagg
               1861 ggtgcccttc tttgggcacc aaagaactag atatagggcg aaatgcgaaa gacttaaaaa
               1921 tcaacaactt aaaaaagggg ggtacgcaac agctcattgc ggcacccccc gcaatagctc
               1981 attgcgtagg ttaaagaaaa tctgtaattg actgccactt ttacgcaacg cataattgtt
               2041 gtcgcgctgc cgaaaagttg cagctgattg cgcatggtgc cgcaaccgtg cggcacccta
               2101 ccgcatggag ataagcatgg ccacgcagtc cagagaaatc ggcattcaag ccaagaacaa
               2161 gcccggtcac tgggtgcaaa cggaacgcaa agcgcatgag gcgtgggccg ggcttattgc
               2221 gaggaaaccc acggcggcaa tgctgctgca tcacctcgtg gcgcagatgg gccaccagaa
               2281 cgccgtggtg gtcagccaga agacactttc caagctcatc ggacgttctt tgcggacggt
               2341 ccaatacgca gtcaaggact tggtggccga gcgctggatc tccgtcgtga agctcaacgg
               2401 ccccggcacc gtgtcggcct acgtggtcaa tgaccgcgtg gcgtggggcc agccccgcga
               2461 ccagttgcgc ctgtcggtgt tcagtgccgc cgtggtggtt gatcacgacg accaggacga
               2521 atcgctgttg gggcatggcg acctgcgccg catcccgacc ctgtatccgg gcgagcagca
               2581 actaccgacc ggccccggcg aggagccgcc cagccagccc ggcattccgg gcatggaacc
               2641 agacctgcca gccttgaccg aaacggagga atgggaacgg cgcgggcagc agcgcctgcc
               2701 gatgcccgat gagccgtgtt ttctggacga tggcgagccg ttggagccgc cgacacgggt
               2761 cacgctgccg cgccggtagt acgtaagagg ttccaacttt caccataatg aaataagatc
               2821 actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgag
               2881 ccatattcaa cgggaaacgt cttgctcgag gccgcgatta aattccaaca tggatgctga
               2941 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg
               3001 attgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc
               3061 caatgatgtt acagatgaga tggtcaggct aaactggctg acggaattta tgcctcttcc
               3121 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc
               3181 agggaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga
               3241 tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa
               3301 cggcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttgg
               3361 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat
               3421 gcataagctt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga
               3481 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat
               3541 cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc
               3601 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca
               3661 gtttcacttg atgctcgatg agtttttctg agggcggatc cccctcaagt caaaagcctc
               3721 cggtcggagg cttttgactt tctgctatgg aggtcaggta tgatttaaat ggtcagtatt
               3781 gagcgatatc tagagaattc gtc
          //
          

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