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      1. <pre id="q7rs8"></pre>
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          pBMT-1

          價格:3000元

          聯系方式:I47-825O-882O

          買家導航

          載體基本信息

          出品人: Lynch,M.D. and Gill,R.T.
          載體名稱: pBMT-1
          質粒類型: 廣宿主載體;克隆載體;原核載體
          高拷貝/低拷貝: --
          克隆方法: 限制性內切酶,多克隆位點
          啟動子:
          載體大小: 3847 bp
          5' 測序引物及序列: --
          3' 測序引物及序列: --
          載體標簽: --
          載體抗性: 氨芐青霉素
          克隆菌株: DH5α 等
          宿主細胞(系): 多個菌屬
          備注: --
          產品目錄號: --
          組成型/誘導型: 組成型

          載體質粒圖譜和多克隆位點信息

          pBMT-1載體圖譜



          載體簡介

          載體序列

          LOCUS       DQ058714                3847 bp    DNA     circular SYN 08-MAY-2006
          DEFINITION  Broad host range vector pBMT-1, complete sequence.
          ACCESSION   DQ058714
          VERSION     DQ058714.1  GI:69048016
          SOURCE      Broad host range vector pBMT-1
          REFERENCE   1  (bases 1 to 3847)
            AUTHORS   Lynch,M.D. and Gill,R.T.
            TITLE     Broad host range vectors for stable genomic library construction
            JOURNAL   Submitted (12-MAY-2005) Chemical & Biological Engineering,
                      University of Colorado, ECCH 111, Campus Box 424, Boulder, CO
                      80309, USA
          FEATURES             Location/Qualifiers
               source          1..3847
                               /organism="Broad host range vector pBMT-1"
                               /mol_type="other DNA"
                               /db_xref="taxon:334946"
               regulatory      60..96
                               /regulatory_class="terminator"
                               /note="soxR terminator"
               gene            complement(104..1123)
                               /gene="mob"
                               /note="mob gene from pBBR1MCS, GenBank Accession Number
                               U02374"
               CDS             complement(104..1123)
                               /gene="mob"
                               /note="required for mobilization"
                               /codon_start=1
                               /transl_table=11
                               /product="Mob"
                               /protein_id="AAY99672.1"
                               /db_xref="GI:69048017"
                               /translation="MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEH
                               WAASSTDEAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWKSASQEQQAAFFEKAHKW
                               LADKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLSAKEFIGNKAQMTRDQTTFAA
                               AVADLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAPQGLAEKLG
                               ISKRVETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVL
                               DALGPLNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPEKCHLTSK
                               KPLLS"
               gene            2117..2779
                               /gene="rep"
                               /note="rep gene from pBBR1, GenBank Accession Number
                               X66730"
               CDS             2117..2779
                               /gene="rep"
                               /note="required for replication"
                               /codon_start=1
                               /transl_table=11
                               /product="Rep"
                               /protein_id="AAY99673.1"
                               /db_xref="GI:69048018"
                               /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLL
                               HHLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAY
                               VVNDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGP
                               GEEPPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLP
                               RR"
               gene            2876..3736
                               /gene="bla"
                               /note="beta-lactam resistance"
               CDS             2876..3736
                               /gene="bla"
                               /codon_start=1
                               /transl_table=11
                               /product="beta-lactamase"
                               /protein_id="AAY99674.1"
                               /db_xref="GI:69048019"
                               /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                               IELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                               YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRL
                               DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSL
                               LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                               EIGASLIKHW"
               regulatory      3752..3783
                               /regulatory_class="terminator"
                               /note="TonB terminator"
          ORIGIN      
                  1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga
                 61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgctcatgat aataatggtt
                121 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg cccgcgttcc tgctggcgct
                181 gggcctgttt ctggcgctgg acttcccgct gttccgtcag cagcttttcg cccacggcct
                241 tgatgatcgc ggcggccttg gcctgcatat cccgattcaa cggccccagg gcgtccagaa
                301 cgggcttcag gcgctcccga aggtctcggg ccgtctcttg ggcttgatcg gccttcttgc
                361 gcatctcacg cgctcctgcg gcggcctgta gggcaggctc atacccctgc cgaaccgctt
                421 ttgtcagccg gtcggccacg gcttccggcg tctcaacgcg ctttgagatt cccagctttt
                481 cggccaatcc ctgcggtgca taggcgcgtg gctcgaccgc ttgcgggctg atggtgacgt
                541 ggcccactgg tggccgctcc agggcctcgt agaacgcctg aatgcgcgtg tgacgtgcct
                601 tgctgccctc gatgccccgt tgcagcccta gatcggccac agcggccgca aacgtggtct
                661 ggtcgcgggt catctgcgct ttgttgccga tgaactcctt ggccgacagc ctgccgtcct
                721 gcgtcagcgg caccacgaac gcggtcatgt gcgggctggt ttcgtcacgg tggatgctgg
                781 ccgtcacgat gcgatccgcc ccgtacttgt ccgccagcca cttgtgcgcc ttctcgaaga
                841 acgccgcctg ctgttcttgg ctggccgact tccaccattc cgggctggcc gtcatgacgt
                901 actcgaccgc caacacagcg tccttgcgcc gcttctctgg cagcaactcg cgcagtcggc
                961 ccatcgcttc atcggtgctg ctggccgccc agtgctcgtt ctctggcgtc ctgctggcgt
               1021 cagcgttggg cgtctcgcgc tcgcggtagg cgtgcttgag actggccgcc acgttgccca
               1081 ttttcgccag cttcttgcat cgcatgatcg cgtatgccgc catgcctgcc cctccctttt
               1141 ggtgtccaac cggctcgacg ggggcagcgc aaggcggtgc ctccggcggg ccactcaatg
               1201 cttgagtata ctcactagac tttgcttcgc aaagtcgtga ccgcctacgg cggctgcggc
               1261 gccctacggg cttgctctcc gggcttcgcc ctgcgcggtc gctgcgctcc cttgccagcc
               1321 cgtggatatg tggacgatgg ccgcgagcgg ccaccggctg gctcgcttcg ctcggcccgt
               1381 ggacaaccct gctggacaag ctgatggaca ggctgcgcct gcccacgagc ttgaccacag
               1441 ggattgccca ccggctaccc agccttcgac cacataccca ccggctccaa ctgcgcggcc
               1501 tgcggccttg ccccatcaat ttttttaatt ttctctgggg aaaagcctcc ggcctgcggc
               1561 ctgcgcgctt cgcttgccgg ttggacacca agtggaaggc gggtcaaggc tcgcgcagcg
               1621 accgcgcagc ggcttggcct tgacgcgcct ggaacgaccc aagcctatgc gagtgggggc
               1681 agtcgaaggc gaagcccgcc cgcctgcccc ccgagcctca cggcggcgag tgcgggggtt
               1741 ccaagggggc agcgccacct tgggcaaggc cgaaggccgc gcagtcgatc aacaagcccc
               1801 ggaggggcca ctttttgccg gagggggagc cgcgccgaag gcgtggggga accccgcagg
               1861 ggtgcccttc tttgggcacc aaagaactag atatagggcg aaatgcgaaa gacttaaaaa
               1921 tcaacaactt aaaaaagggg ggtacgcaac agctcattgc ggcacccccc gcaatagctc
               1981 attgcgtagg ttaaagaaaa tctgtaattg actgccactt ttacgcaacg cataattgtt
               2041 gtcgcgctgc cgaaaagttg cagctgattg cgcatggtgc cgcaaccgtg cggcacccta
               2101 ccgcatggag ataagcatgg ccacgcagtc cagagaaatc ggcattcaag ccaagaacaa
               2161 gcccggtcac tgggtgcaaa cggaacgcaa agcgcatgag gcgtgggccg ggcttattgc
               2221 gaggaaaccc acggcggcaa tgctgctgca tcacctcgtg gcgcagatgg gccaccagaa
               2281 cgccgtggtg gtcagccaga agacactttc caagctcatc ggacgttctt tgcggacggt
               2341 ccaatacgca gtcaaggact tggtggccga gcgctggatc tccgtcgtga agctcaacgg
               2401 ccccggcacc gtgtcggcct acgtggtcaa tgaccgcgtg gcgtggggcc agccccgcga
               2461 ccagttgcgc ctgtcggtgt tcagtgccgc cgtggtggtt gatcacgacg accaggacga
               2521 atcgctgttg gggcatggcg acctgcgccg catcccgacc ctgtatccgg gcgagcagca
               2581 actaccgacc ggccccggcg aggagccgcc cagccagccc ggcattccgg gcatggaacc
               2641 agacctgcca gccttgaccg aaacggagga atgggaacgg cgcgggcagc agcgcctgcc
               2701 gatgcccgat gagccgtgtt ttctggacga tggcgagccg ttggagccgc cgacacgggt
               2761 cacgctgccg cgccggtagt acgtaagagg ttccaacttt caccataatg aaataagatc
               2821 actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgag
               2881 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
               2941 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
               3001 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagtttac gccccgaaga
               3061 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
               3121 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
               3181 gtactcacca gtcacagaaa agcatctcac ggatggcatg acagtaagag aattatgcag
               3241 tgctgccata accatgagtg ataacactgc ggccaactta cttctggcaa cgatcggagg
               3301 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
               3361 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
               3421 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
               3481 gcaacaatta atagactgga tggaggcgga taaagttgca ggatcacttc tgcgctcggc
               3541 cctcccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
               3601 tatcattgca gcactggggc cagatggtaa gccctcccgc atcgtagtta tctacacgac
               3661 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
               3721 gattaagcat tggtaatgag gatccccctc aagtcaaaag cctccggtcg gaggcttttg
               3781 actttctgct atggaggtca ggtatgattt aaatggtcag tattgagcga tatctagaga
               3841 attcgtc
          //
          
          
          

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