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          pBBR1MCS-5

          價格:3000元

          聯系方式:I47-825O-882O

          買家導航

          pBBRMCS5載體基本信息

          出品公司: --
          載體名稱: pBBR1MCS-5, pBBR1MCS5, pBBRMCS5
          質粒類型: 廣宿主蛋白表達或克隆載體質粒,廣宿主載體質粒
          高拷貝/低拷貝: 高拷貝
          克隆方法: 限制性內切酶,多克隆位點
          啟動子: --
          載體大小: 4.7 kb
          5' 測序引物及序列: M13-F: TGTAAAACGACGGCCAGT
          3' 測序引物及序列: M13-R: CAGGAAACAGCTATGAC
          載體標簽: --
          載體抗性: 慶大霉素
          篩選標記: --
          克隆菌株: DH5α 等
          宿主細胞(系): 多種微生物物種,是廣宿主質粒。
          備注: 參考文獻:
          [1] Kovach ME, Phillips RW, Elzer PH, Roop RM, 2nd, Peterson KM. pBBR1MCS: a broad-host-range cloning vector. BioTechniques 1994;16:800-2.

          [2] Kovach ME, Elzer PH, Hill DS, Robertson GT, Farris MA, Roop RM, 2nd, et al. Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes. Gene 1995;166:175-6.

          GenBank sequence:XXU25061
          產品目錄號: --
          穩定性/瞬表達: 穩表達
          組成型/誘導型: 組成型
          病毒/非病毒: 非病毒

          pBBRMCS5載體質粒圖譜和多克隆位點信息

          pBBR1MCS-5載體圖譜


          pBBRMCS5載體圖譜


          pBBR1MCS5載體圖譜

          pBBRMCS5載體簡介

          pBBRMCS5載體序列

          LOCUS       pBBR1MCS-5              4768 bp    DNA     linear   SYN 10-JAN-2005
          DEFINITION  Cloning vector pBBR1MCS-5 REP, LacZ alpha peptide (lacZ alpha), and
                      gentamicin-3-acetyltransferase genes, complete cds.
          ACCESSION   U25061
          VERSION     U25061.1  GI:833825
          KEYWORDS    .
          SOURCE      Cloning vector pBBR1MCS-5
            ORGANISM  Cloning vector pBBR1MCS-5
                      other sequences; artificial sequences; vectors.
          COMMENT     This file is created by Vector NTI
                      http://www.ningxiaxinwen.com/
          COMMENT     ORIGDB|GenBank
          COMMENT     VNTAUTHORNAME|biofeng.com|
          REFERENCE   1  (bases 1 to 4768)
            AUTHORS   Kovach,M.E., Phillips,R.W., Elzer,P.H., Roop,R.M. II and
                      Peterson,K.M.
            TITLE     pBBR1MCS: a broad-host-range cloning vector
            JOURNAL   BioTechniques 16 (5), 800-802 (1994)
             PUBMED   8068328
          REFERENCE   2  (bases 1 to 4768)
            AUTHORS   Kovach,M.E., Elzer,P.H., Hill,D.S., Robertson,G.T., Farris,M.A.,
                      Roop,R.M. II and Peterson,K.M.
            TITLE     Four new derivatives of the broad-host-range cloning vector
                      pBBR1MCS, carrying different antibiotic-resistance cassettes
            JOURNAL   Gene 166 (1), 175-176 (1995)
             PUBMED   8529885
          REFERENCE   3  (bases 1 to 4768)
            AUTHORS   Kovach,M.E.
            TITLE     Direct Submission
            JOURNAL   Submitted (13-APR-1995) Michael E. Kovach, Microbiology, Louisiana
                      State University, 1501 Kings Highway, Shreveport, LA 71130, USA
          FEATURES             Location/Qualifiers
               source          1..4768
                               /organism="Cloning vector pBBR1MCS-5"
                               /mol_type="genomic DNA"
                               /db_xref="taxon:51200"
                               /lab_host="Bordetella bronchiseptica"
               CDS             1793..2455
                               /note="required for plasmid replication"
                               /codon_start=1
                               /transl_table=11
                               /product="REP"
                               /protein_id="AAB06694.1"
                               /db_xref="GI:833826"
               gene            complement(3013..3378)
                               /gene="lacZ alpha"
               CDS             complement(3013..3378)
                               /gene="lacZ alpha"
                               /codon_start=1
                               /transl_table=11
                               /product="LacZ alpha peptide"
                               /protein_id="AAB06695.1"
                               /db_xref="GI:833827"
               CDS             3975..4508
                               /function="resistance to gentamicin"
                               /codon_start=1
                               /transl_table=11
                               /product="gentamicin-3-acetyltransferase"
                               /protein_id="AAB06696.1"
                               /db_xref="GI:833828"
          ORIGIN      
                  1 ctcgggccgt ctcttgggct tgatcggcct tcttgcgcat ctcacgcgct cctgcggcgg
                 61 cctgtagggc aggctcatac ccctgccgaa ccgcttttgt cagccggtcg gccacggctt
                121 ccggcgtctc aacgcgcttt gagattccca gcttttcggc caatccctgc ggtgcatagg
                181 cgcgtggctc gaccgcttgc gggctgatgg tgacgtggcc cactggtggc cgctccaggg
                241 cctcgtagaa cgcctgaatg cgcgtgtgac gtgccttgct gccctcgatg ccccgttgca
                301 gccctagatc ggccacagcg gccgcaaacg tggtctggtc gcgggtcatc tgcgctttgt
                361 tgccgatgaa ctccttggcc gacagcctgc cgtcctgcgt cagcggcacc acgaacgcgg
                421 tcatgtgcgg gctggtttcg tcacggtgga tgctggccgt cacgatgcga tccgccccgt
                481 acttgtccgc cagccacttg tgcgccttct cgaagaacgc cgcctgctgt tcttggctgg
                541 ccgacttcca ccattccggg ctggccgtca tgacgtactc gaccgccaac acagcgtcct
                601 tgcgccgctt ctctggcagc aactcgcgca gtcggcccat cgcttcatcg gtgctgctgg
                661 ccgcccagtg ctcgttctct ggcgtcctgc tggcgtcagc gttgggcgtc tcgcgctcgc
                721 ggtaggcgtg cttgagactg gccgccacgt tgcccatttt cgccagcttc ttgcatcgca
                781 tgatcgcgta tgccgccatg cctgcccctc ccttttggtg tccaaccggc tcgacggggg
                841 cagcgcaagg cggtgcctcc ggcgggccac tcaatgcttg agtatactca ctagactttg
                901 cttcgcaaag tcgtgaccgc ctacggcggc tgcggcgccc tacgggcttg ctctccgggc
                961 ttcgccctgc gcggtcgctg cgctcccttg ccagcccgtg gatatgtgga cgatggccgc
               1021 gagcggccac cggctggctc gcttcgctcg gcccgtggac aaccctgctg gacaagctga
               1081 tggacaggct gcgcctgccc acgagcttga ccacagggat tgcccaccgg ctacccagcc
               1141 ttcgaccaca tacccaccgg ctccaactgc gcggcctgcg gccttgcccc atcaattttt
               1201 ttaattttct ctggggaaaa gcctccggcc tgcggcctgc gcgcttcgct tgccggttgg
               1261 acaccaagtg gaaggcgggt caaggctcgc gcagcgaccg cgcagcggct tggccttgac
               1321 gcgcctggaa cgacccaagc ctatgcgagt gggggcagtc gaaggcgaag cccgcccgcc
               1381 tgccccccga gcctcacggc ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg
               1441 caaggccgaa ggccgcgcag tcgatcaaca agccccggag gggccacttt ttgccggagg
               1501 gggagccgcg ccgaaggcgt gggggaaccc cgcaggggtg cccttctttg ggcaccaaag
               1561 aactagatat agggcgaaat gcgaaagact taaaaatcaa caacttaaaa aaggggggta
               1621 cgcaacagct cattgcggca ccccccgcaa tagctcattg cgtaggttaa agaaaatctg
               1681 taattgactg ccacttttac gcaacgcata attgttgtcg cgctgccgaa aagttgcagc
               1741 tgattgcgca tggtgccgca accgtgcggc accctaccgc atggagataa gcatggccac
               1801 gcagtccaga gaaatcggca ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga
               1861 acgcaaagcg catgaggcgt gggccgggct tattgcgagg aaacccacgg cggcaatgct
               1921 gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca gccagaagac
               1981 actttccaag ctcatcggac gttctttgcg gacggtccaa tacgcagtca aggacttggt
               2041 ggccgagcgc tggatctccg tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt
               2101 ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag
               2161 tgccgccgtg gtggttgatc acgacgacca ggacgaatcg ctgttggggc atggcgacct
               2221 gcgccgcatc ccgaccctgt atccgggcga gcagcaacta ccgaccggcc ccggcgagga
               2281 gccgcccagc cagcccggca ttccgggcat ggaaccagac ctgccagcct tgaccgaaac
               2341 ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc cgtgttttct
               2401 ggacgatggc gagccgttgg agccgccgac acgggtcacg ctgccgcgcc ggtagcactt
               2461 gggttgcgca gcaacccgta agtgcgctgt tccagactat cggctgtagc cgcctcgccg
               2521 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga
               2581 cctgaatgga agccggcggc acctcgctaa cggattcacc gtttttatca ggctctggga
               2641 ggcagaataa atgatcatat cgtcaattat tacctccacg gggagagcct gagcaaactg
               2701 gcctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa
               2761 ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcgaa
               2821 tttgctttcg aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaccag
               2881 gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc
               2941 agtcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
               3001 aaatattaac gcttacaatt tccattcgcc attcaggctg cgcaactgtt gggaagggcg
               3061 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
               3121 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
               3181 gcgcgcgtaa tacgactcac tatagggcga attggagctc caccgcggtg gcggccgctc
               3241 tagaactagt ggatcccccg ggctgcagga attcgatatc aagcttatcg ataccgtcga
               3301 cctcgagggg gggcccggta cccagctttt gttcccttta gtgagggtta attgcgcgct
               3361 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
               3421 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
               3481 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
               3541 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgcatgcata
               3601 aaaactgttg taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat
               3661 gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg
               3721 acgcacaccg tggaaacgga tgaaggcacg aacccagttg acataagcct gttcggttcg
               3781 taaactgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg
               3841 cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttgtaca
               3901 gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt
               3961 atggagcagc aacgatgtta cgcagcagca acgatgttac gcagcagggc agtcgcccta
               4021 aaacaaagtt aggtggctca agtatgggca tcattcgcac atgtaggctc ggccctgacc
               4081 aagtcaaatc catgcgggct gctcttgatc ttttcggtcg tgagttcgga gacgtagcca
               4141 cctactccca acatcagccg gactccgatt acctcgggaa cttgctccgt agtaagacat
               4201 tcatcgcgct tgctgccttc gaccaagaag cggttgttgg cgctctcgcg gcttacgttc
               4261 tgcccaggtt tgagcagccg cgtagtgaga tctatatcta tgatctcgca gtctccggcg
               4321 agcaccggag gcagggcatt gccaccgcgc tcatcaatct cctcaagcat gaggccaacg
               4381 cgcttggtgc ttatgtgatc tacgtgcaag cagattacgg tgacgatccc gcagtggctc
               4441 tctatacaaa gttgggcata cgggaagaag tgatgcactt tgatatcgac ccaagtaccg
               4501 ccacctaaca attcgttcaa gccgagatcg gcttcccggc cgcggagttg ttcggtaaat
               4561 tgtcacaacg ccgccaggtg gcacttttcg gggaaatgtg cgcgcccgcg ttcctgctgg
               4621 cgctgggcct gtttctggcg ctggacttcc cgctgttccg tcagcagctt ttcgcccacg
               4681 gccttgatga tcgcggcggc cttggcctgc atatcccgat tcaacggccc cagggcgtcc
               4741 agaacgggct tcaggcgctc ccgaaggt
          //
          

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