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          Antimycotic Sensitivity Test Agar

          價格:

          規格: 250g 500g

          聯系方式:I47-825O-882O

          買家導航

          抗真菌藥物敏感性試驗瓊脂培養基產品基本信息

          產品名稱: 抗真菌藥物敏感性試驗瓊脂;抗真菌劑敏感性試驗培養基
          英文名稱: Antimycotic Sensitivity Test Agar
          培養基類型: 非選擇性培養基
          級別: for microbiology
          品牌: ELITE-MEDIA(艾禮培養基)
          產品目錄號: M298-01、M298-02
          產品規格: 250g、500g
          產品外觀: 麥秸色粉末。
          滅菌后顏色與澄清度: 琥珀色略不透明凝膠。
          保存條件: 密封,2-25°C保存。
          注意事項: 避免攝入、吸入、皮膚接觸。
          相關產品: --
          艾禮培養基


          產品描述:

          抗真菌藥物敏感試驗瓊脂(Antimycotic Sensitivity Test Agar)用于擴散法檢測抗真菌藥物敏感性試驗。


          工作原理

          真菌感染在過去的十年中急劇增加,經常伴隨系統性感染,如AIDS、癌癥患者和免疫能力降低的病人。不 幸的是,抗真菌藥物種類很少,真菌感染趨于快速產生對這些藥物的耐藥性。真菌感染的致死率遠高于細菌感染。因此,抗真菌劑的增加和抗 真菌劑的需求的增加,急需更快、更準確的抗真菌藥物篩選方法和藥敏試驗(susceptibility testing)方法?拐婢鷦y定瓊脂由Berger 和Lazecka研發,既作為基礎瓊脂也作為種子瓊脂,用于便捷地測定藥品和其它材料的抗真菌活性。該培養基是合成培養基,成分是已知的, 因此抑制性只能來自抗真菌劑,而不是養分耗竭。



          用途:

          抗真菌藥物敏感性試驗瓊脂被推薦用于測定藥品和其它材料的抗真菌活性


          配方與配制方法


          成分 g/L
          酪蛋白胨 19
          酵母提取物 10
          葡萄糖 20
          檸檬酸鈉 10
          磷酸氫二鈉 1
          瓊脂 25
          pH 6.6±0.2  

          配制方法:
          1. 稱取85g本產品,用1000ml純水重懸。
          2. 加熱使培養基完全溶解。
          3. 121°C 滅菌15min。
          4. 冷卻至45-50°C 時倒平板。


          實驗方法

          Preparation of Inoculum:

          Inoculum is prepared by picking five distinct colonies of approximately 1mm from 24 hours old culture grown on Sabouraud Dextrose Agar and incubated at 35 ± 2°C. Colonies are suspended in 5ml of sterile 0.85% Saline. Vortex the resulting suspension and adjust the turbidity to yield 1 x 106 - 5 x 106 cells /ml (i.e. 0.5 McFarland standard).

          Test Procedure:

          1. Prepare plates with Antimycotic sensitivity Test Agar for carrying out susceptibility of antifungal discs. The medium in the plates should be sterile and have a depth of about 4 mm.
          2. Dip a sterile non-toxic cotton swab on a wooden applicator into the standardized inoculum (turbidity so adjusted, as to obtain semi confluent growth on the petri plate) and rotate the soaked swab firmly against the upper inside wall of the tube to express excess fluid. Streak the entire agar surface of the plate with the swab three times, turning the plate at 60° angle between each streaking. Allow the inoculum to dry for 5 - 15 minutes with lid in place.
          3. Apply the discs using aseptic technique. Deposit the discs with centers at least 24 mm apart.
          4. Invert the plates and place in an incubator set to 35 ± 2°C within 15 minutes after the discs are applied.
          5. Examine each plate after 20 - 24 hours of incubation. If plate was satisfactorily streaked the resulting zones of inhibition will be uniformly circular and there will be a semi-confluent lawn of growth. Read at 48 hours only when insufficient growth is observed after 24 hours incubation.


          Antimycotic Sensitivity Test Agar is recommended for testing the antimycotic activity by disc diffusion method (Sensitivity disc or MIC testing). The Clinical and Laboratory Standards Institute (CLSI) subcommittee on Antifungal Susceptibility Testing has established interpretative break point for three drugs and Candida spp. The M44-A document (approved standard) became available in 2004. Espinel-Ingroff suggested that easier test modification to CLSI methods are desirable . Several workers have used agar diffusion method as an alternative approch to the CLSI methods. Stiller et al observed a good correlation between MICs and growth inhibition zones for 5-FC. Pfaller et al found a fluconazole disk test to be comparable to the MIC test. Barry and Brown demonstrated good correlation between fluconazole disk test and MIC determined by either broth dilution or E Test. Espinel et al and Pfaller et al evaluated E Test and suggested that it correlates well with CLSI reference methods. However this agreement was species and medium dependent and they suggested the need for further optimisation of medium formulation.

          抗真菌藥物敏感性試驗瓊脂培養基產品說明書pdf版和相關資料下載

          抗真菌藥物敏感性試驗瓊脂培養基產品應用舉例

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